Construct: ORF TRCN0000488894
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020981.1_s317c1
- DNA Barcode:
- ACTAGAGTGGGAGCGTGAGCAAAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ADRA1D (146)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488894
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 146 | ADRA1D | adrenoceptor alpha 1D | NM_000678.4 | 99.9% | 100% | 141G>A |
| 2 | mouse | 11550 | Adra1d | adrenergic receptor, alpha 1d | NM_013460.4 | 82% | 85.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1788
- ORF length:
- 1716
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgactttc cgcgatctcc tgagcgtcag tttcgaggga ccccgcccgg 121 acagcagcgc agggggctcc agcgcgggcg gcggcggggg cagcgcgggc ggcgcggccc 181 cctcggaggg cccggcggtg ggcggcgtgc cagggggcgc gggcggcggc ggcggcgtgg 241 tgggcgcagg cagcggcgag gacaaccgga gctccgcggg ggagccgggg agcgcgggcg 301 cgggcggcga cgtgaatggc acggcggccg tcgggggact ggtggtgagc gcgcagggcg 361 tgggcgtggg cgtcttcctg gcagccttca tccttatggc cgtggcaggt aacctgcttg 421 tcatcctctc agtggcctgc aaccgccacc tgcagaccgt caccaactat ttcatcgtga 481 acctggccgt ggccgacctg ctgctgagcg ccaccgtact gcccttctcg gccaccatgg 541 aggttctggg cttctgggcc tttggccgcg ccttctgcga cgtatgggcc gccgtggacg 601 tgctgtgctg cacggcctcc atcctcagcc tctgcaccat ctccgtggac cggtacgtgg 661 gcgtgcgcca ctcactcaag tacccagcca tcatgaccga gcgcaaggcg gccgccatcc 721 tggccctgct ctgggtcgta gccctggtgg tgtccgtagg gcccctgctg ggctggaagg 781 agcccgtgcc ccctgacgag cgcttctgcg gtatcaccga ggaggcgggc tacgctgtct 841 tctcctccgt gtgctccttc tacctgccca tggcggtcat cgtggtcatg tactgccgcg 901 tgtacgtggt cgcgcgcagc accacgcgca gcctcgaggc gggcgtcaag cgcgagcgag 961 gcaaggcctc cgaggtggtg ctgcgcatcc actgtcgcgg cgcggccacg ggcgccgacg 1021 gggcgcacgg catgcgcagc gccaagggcc acaccttccg cagctcgctc tccgtgcgcc 1081 tgctcaagtt ctcccgtgag aagaaagcgg ccaagactct ggccatcgtc gtgggtgtct 1141 tcgtgctctg ctggttccct ttcttctttg tcctgccgct cggctccttg ttcccgcagc 1201 tgaagccatc ggagggcgtc ttcaaggtca tcttctggct cggctacttc aacagctgcg 1261 tgaacccgct catctacccc tgttccagcc gcgagttcaa gcgcgccttc ctccgtctcc 1321 tgcgctgcca gtgccgtcgt cgccggcgcc gccgccctct ctggcgtgtc tacggccacc 1381 actggcgggc ctccaccagc ggcctgcgcc aggactgcgc cccgagttcg ggcgacgcgc 1441 cccccggagc gccgctggcc ctcaccgcgc tccccgaccc cGACCCCGAA CCCCCAGGCA 1501 CGCCCGAGAT GCAGGCTCCG GTCGCCAGCC GTCGAAAGCC ACCCAGCGCC TTCCGCGAGT 1561 GGAGGCTGCT GGGGCCGTTC CGGAGACCCA CGACCCAGCT GCGCGCCAAA GTCTCCAGCC 1621 TGTCGCACAA GATCCGCGCC GGGGGCGCGC AGCGCGCAGA GGCAGCGTGC GCCCAGCGCT 1681 CAGAGGTGGA GGCTGTGTCC CTAGGCGTCC CACACGAGGT GGCCGAGGGC GCCACCTGCC 1741 AGGCCTACGA ATTGGCCGAC TACAGCAACC TACGGGAGAC CGATATTTAA GACCCAGCTT 1801 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1861 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1921 CTTGTGGAAA GGACGAACTA GAGTGGGAGC GTGAGCAAAT ACGCGTTAAG TCgacaatca 1981 acctctggat tacaaaattt gtgaaagatt