Construct: ORF TRCN0000488901

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020448.1_s317c1
DNA Barcode:
CGTAATTCACATTTTCAAATGTAG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
EIF2AK2 (5610)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488901
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5610 EIF2AK2 eukaryotic translation init... NM_001135651.3 100% 100%
2 human 5610 EIF2AK2 eukaryotic translation init... NM_002759.3 100% 100%
3 human 5610 EIF2AK2 eukaryotic translation init... XM_011532987.2 100% 100%
4 human 5610 EIF2AK2 eukaryotic translation init... NM_001135652.2 92.5% 92.5% 785_786ins123
5 human 5610 EIF2AK2 eukaryotic translation init... XM_017004503.1 92.1% 92.1% 1246_1247ins129
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1725
ORF length:
1653
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggctggt gatctttcag caggtttctt catggaggaa cttaatacat
121accgtcagaa gcagggagta gtacttaaat atcaagaact gcctaattca ggacctccac
181atgataggag gtttacattt caagttataa tagatggaag agaatttcca gaaggtgaag
241gtagatcaaa gaaggaagca aaaaatgccg cagccaaatt agctgttgag atacttaata
301aggaaaagaa ggcagttagt cctttattat tgacaacaac gaattcttca gaaggattat
361ccatggggaa ttacataggc cttatcaata gaattgccca gaagaaaaga ctaactgtaa
421attatgaaca gtgtgcatcg ggggtgcatg ggccagaagg atttcattat aaatgcaaaa
481tgggacagaa agaatatagt attggtacag gttctactaa acaggaagca aaacaattgg
541ccgctaaact tgcatatctt cagatattat cagaagaaac ctcagtgaaa tctgactacc
601tgtcctctgg ttcttttgct actacgtgtg agtcccaaag caactcttta gtgaccagca
661cactcgcttc tgaatcatca tctgaaggtg acttctcagc agatacatca gagataaatt
721ctaacagtga cagtttaaac agttcttcgt tgcttatgaa tggtctcaga aataatcaaa
781ggaaggcaaa aagatctttg gcacccagat ttgaccttcc tgacatgaaa gaaacaaagt
841atactgtgga caagaggttt ggcatggatt ttaaagaaat agaattaatt ggctcaggtg
901gatttggcca agttttcaaa gcaaaacaca gaattgacgg aaagacttac gttattaaac
961gtgttaaata taataacgag aaggcggagc gtgaagtaaa agcattggca aaacttgatc
1021atgtaaatat tgttcactac aatggctgtt gggatggatt tgattatgat cctgagacca
1081gtgatgattc tcttgagagc agtgattatg atcctgagaa cagcaaaaat agttcaaggt
1141caaagactaa gtgccttttc atccaaatgg aattctgtga taaagggacc ttggaacaat
1201ggattgaaaa aagaagaggc gagaaactag acaaagtttt ggctttggaa ctctttgaac
1261aaataacaaa aggggtggat tatatacatt caaaaaaatt aattcataga gatcttaagc
1321caagtaatat attcttagta gatacaaaac aagtaaagat tggagacttt ggacttgtaa
1381catctcTGAA AAATGATGGA AAGCGAACAA GGAGTAAGGG AACTTTGCGA TACATGAGCC
1441CAGAACAGAT TTCTTCGCAA GACTATGGAA AGGAAGTGGA CCTCTACGCT TTGGGGCTAA
1501TTCTTGCTGA ACTTCTTCAT GTATGTGACA CTGCTTTTGA AACATCAAAG TTTTTCACAG
1561ACCTACGGGA TGGCATCATC TCAGATATAT TTGATAAAAA AGAAAAAACT CTTCTACAGA
1621AATTACTCTC AAAGAAACCT GAGGATCGAC CTAACACATC TGAAATACTA AGGACCTTGA
1681CTGTGTGGAA GAAAAGCCCA GAGAAAAATG AACGACACAC ATGTTAGGAC CCAGCTTTCT
1741TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
1801CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
1861GTGGAAAGGA CGACGTAATT CACATTTTCA AATGTAGACG CGTTAAGTCg acaatcaacc
1921tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)

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