Construct: ORF TRCN0000488901
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020448.1_s317c1
- DNA Barcode:
- CGTAATTCACATTTTCAAATGTAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- EIF2AK2 (5610)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488901
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5610 | EIF2AK2 | eukaryotic translation init... | NM_001135651.3 | 100% | 100% | |
| 2 | human | 5610 | EIF2AK2 | eukaryotic translation init... | NM_002759.3 | 100% | 100% | |
| 3 | human | 5610 | EIF2AK2 | eukaryotic translation init... | XM_011532987.2 | 100% | 100% | |
| 4 | human | 5610 | EIF2AK2 | eukaryotic translation init... | NM_001135652.2 | 92.5% | 92.5% | 785_786ins123 |
| 5 | human | 5610 | EIF2AK2 | eukaryotic translation init... | XM_017004503.1 | 92.1% | 92.1% | 1246_1247ins129 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1725
- ORF length:
- 1653
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggctggt gatctttcag caggtttctt catggaggaa cttaatacat 121 accgtcagaa gcagggagta gtacttaaat atcaagaact gcctaattca ggacctccac 181 atgataggag gtttacattt caagttataa tagatggaag agaatttcca gaaggtgaag 241 gtagatcaaa gaaggaagca aaaaatgccg cagccaaatt agctgttgag atacttaata 301 aggaaaagaa ggcagttagt cctttattat tgacaacaac gaattcttca gaaggattat 361 ccatggggaa ttacataggc cttatcaata gaattgccca gaagaaaaga ctaactgtaa 421 attatgaaca gtgtgcatcg ggggtgcatg ggccagaagg atttcattat aaatgcaaaa 481 tgggacagaa agaatatagt attggtacag gttctactaa acaggaagca aaacaattgg 541 ccgctaaact tgcatatctt cagatattat cagaagaaac ctcagtgaaa tctgactacc 601 tgtcctctgg ttcttttgct actacgtgtg agtcccaaag caactcttta gtgaccagca 661 cactcgcttc tgaatcatca tctgaaggtg acttctcagc agatacatca gagataaatt 721 ctaacagtga cagtttaaac agttcttcgt tgcttatgaa tggtctcaga aataatcaaa 781 ggaaggcaaa aagatctttg gcacccagat ttgaccttcc tgacatgaaa gaaacaaagt 841 atactgtgga caagaggttt ggcatggatt ttaaagaaat agaattaatt ggctcaggtg 901 gatttggcca agttttcaaa gcaaaacaca gaattgacgg aaagacttac gttattaaac 961 gtgttaaata taataacgag aaggcggagc gtgaagtaaa agcattggca aaacttgatc 1021 atgtaaatat tgttcactac aatggctgtt gggatggatt tgattatgat cctgagacca 1081 gtgatgattc tcttgagagc agtgattatg atcctgagaa cagcaaaaat agttcaaggt 1141 caaagactaa gtgccttttc atccaaatgg aattctgtga taaagggacc ttggaacaat 1201 ggattgaaaa aagaagaggc gagaaactag acaaagtttt ggctttggaa ctctttgaac 1261 aaataacaaa aggggtggat tatatacatt caaaaaaatt aattcataga gatcttaagc 1321 caagtaatat attcttagta gatacaaaac aagtaaagat tggagacttt ggacttgtaa 1381 catctcTGAA AAATGATGGA AAGCGAACAA GGAGTAAGGG AACTTTGCGA TACATGAGCC 1441 CAGAACAGAT TTCTTCGCAA GACTATGGAA AGGAAGTGGA CCTCTACGCT TTGGGGCTAA 1501 TTCTTGCTGA ACTTCTTCAT GTATGTGACA CTGCTTTTGA AACATCAAAG TTTTTCACAG 1561 ACCTACGGGA TGGCATCATC TCAGATATAT TTGATAAAAA AGAAAAAACT CTTCTACAGA 1621 AATTACTCTC AAAGAAACCT GAGGATCGAC CTAACACATC TGAAATACTA AGGACCTTGA 1681 CTGTGTGGAA GAAAAGCCCA GAGAAAAATG AACGACACAC ATGTTAGGAC CCAGCTTTCT 1741 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1801 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1861 GTGGAAAGGA CGACGTAATT CACATTTTCA AATGTAGACG CGTTAAGTCg acaatcaacc 1921 tctggattac aaaatttgtg aaagatt