Construct: ORF TRCN0000488911
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019168.1_s317c1
- DNA Barcode:
- TCCCCCAAACGGTCCCGAGCAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RGR (5995)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488911
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5995 | RGR | retinal G protein coupled r... | NM_002921.3 | 99.5% | 99.6% | (many diffs) |
| 2 | human | 5995 | RGR | retinal G protein coupled r... | NM_001012720.2 | 98.1% | 98.3% | (many diffs) |
| 3 | human | 5995 | RGR | retinal G protein coupled r... | NM_001012722.1 | 85.3% | 85.4% | (many diffs) |
| 4 | human | 5995 | RGR | retinal G protein coupled r... | XM_024448118.1 | 71.4% | 70.9% | (many diffs) |
| 5 | human | 5995 | RGR | retinal G protein coupled r... | XR_002957005.1 | 30.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 963
- ORF length:
- 888
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggca gagaccagtg ccttgcccac cggcttcggg gagctcgagg 121 tgctggctgt ggggatggtg ctactggtgg aagctctctc cggtctcagc ctcaataccc 181 tgaccatctt ctctttctgc aagaccccgg agctgcggac tccctgccac ctactggtgc 241 tgagcttggc tcttgcggac agtgggatca gcctgaatgc cctcgttgca gccacatcca 301 gccttctccg tgtctcccac aggcgctggc cctacggctc ggacggctgc caggctcacg 361 gcttccaggg ctttgtgaca gcgttggcca gcatctgcag cagtgcagcc atcgcatggg 421 ggcgttatca ccactactgc acccgtagcc agctggcctg gaactcagcc gtctctctgg 481 tgctcttcgt gtggctgtct tctgccttct gggcagctct gccccttctg ggttggggtc 541 actatgacta tgagccactg gggacatgct gcaccctgga ctactccaag ggggacagaa 601 acttcaccag cttcctcttc accatgtccT TCTTCAACTT CGCCATGCCC CTCTTCATCA 661 CGATCACTTC CTACAGTCTC ATGGAGCAGA AACTGGGGAA GAGTGGCCAT CTCCAGGTAA 721 ACACCACTCT GCCAGCAAGG ACGCTGCTGC TCGGCTGGGG CCCCTATGCC ATCCTGTATC 781 TATACGCAGT CATCGCAGAC GTGACTTCCA TCTCCCCCAA ACTGCAGATG GTGCCCGCCC 841 TCATTGCCAA AATGGTGCCC ACGATCAATG CCATCAACTA TGCCCTGGGC AATGAGATGG 901 TCTGCAGGGG AATCTGGCAG TGCCTCTCAC CGCAGAAGAG GGAGAAGGAC CGAACCAAGG 961 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1021 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1081 TTTATATATC TTGTGGAAAG GACGATCCCC CAAACGGTCC CGAGCAAATA CGCGTTAAGT 1141 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt