Construct: ORF TRCN0000488921
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021908.1_s317c1
- DNA Barcode:
- AAGTCAGACCCAGGGTTGAACCGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HMGN1 (3150)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488921
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3150 | HMGN1 | high mobility group nucleos... | NM_004965.7 | 100% | 100% | |
| 2 | human | 3150 | HMGN1 | high mobility group nucleos... | XM_011529543.2 | 28.2% | 4.4% | 0_1ins123;2_15del;192_504del |
| 3 | human | 3150 | HMGN1 | high mobility group nucleos... | XM_011529544.2 | 28.2% | 4.4% | 0_1ins123;2_15del;192_504del |
| 4 | human | 3150 | HMGN1 | high mobility group nucleos... | XM_011529549.2 | 28.2% | 4.4% | 0_1ins123;2_15del;192_504del |
| 5 | human | 3150 | HMGN1 | high mobility group nucleos... | XM_017028333.2 | 28.2% | 4.4% | 0_1ins123;2_15del;192_504del |
| 6 | human | 3150 | HMGN1 | high mobility group nucleos... | XM_017028334.1 | 28.2% | 4.4% | 0_1ins123;2_15del;192_504del |
| 7 | human | 3150 | HMGN1 | high mobility group nucleos... | XM_017028335.2 | 28.2% | 4.4% | 0_1ins123;2_15del;192_504del |
| 8 | human | 3150 | HMGN1 | high mobility group nucleos... | XM_017028336.2 | 28.2% | 4.4% | 0_1ins123;2_15del;192_504del |
| 9 | human | 3150 | HMGN1 | high mobility group nucleos... | XM_024452067.1 | 28.2% | 4.4% | 0_1ins123;2_15del;192_504del |
| 10 | human | 3150 | HMGN1 | high mobility group nucleos... | XM_024452068.1 | 28.2% | 4.4% | 0_1ins123;2_15del;192_504del |
| 11 | human | 3150 | HMGN1 | high mobility group nucleos... | XM_024452069.1 | 28.2% | 4.4% | 0_1ins123;2_15del;192_504del |
| 12 | human | 3150 | HMGN1 | high mobility group nucleos... | XM_024452070.1 | 28.2% | 4.4% | 0_1ins123;2_15del;192_504del |
| 13 | human | 3150 | HMGN1 | high mobility group nucleos... | XM_024452071.1 | 28.2% | 4.4% | 0_1ins123;2_15del;192_504del |
| 14 | human | 10230 | NBR2 | neighbor of BRCA1 lncRNA 2 | NR_003108.2 | 16.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 393
- ORF length:
- 300
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGCAGGCA CCATGCCCAA GAGGAAGGTC AGCTCCGCCG 121 AAGGCGCCGC CAAGGAAGAG CCCAAGAGGA GATCGGCGCG GTTGTCAGCT AAACCTCCTG 181 CAAAAGTGGA AGCGAAGCCG AAAAAGGCAG CAGCGAAGGA TAAATCTTCA GACAAAAAAG 241 TGCAAACAAA AGGGAAAAGG GGAGCAAAGG GAAAACAGGC CGAAGTGGCT AACCAAGAAA 301 CTAAAGAAGA CTTACCTGCG GAAAACGGGG AAACGAAGAC TGAGGAGAGT CCAGCCTCTG 361 ATGAAGCAGG AGAGAAAGAA GCCAAGTCTG ATTAGAACCC AGCTTTCTTG TACAAAGTGG 421 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 481 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 541 AAAGTCAGAC CCAGGGTTGA ACCGAACGCG TTAAGTCgac aatcaacctc tggattacaa 601 aatttgtgaa agatt