Construct: ORF TRCN0000488934

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021134.1_s317c1
DNA Barcode:
ATCCCTTTCGACCTCTAAACCATC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
ADGRF5 (221395)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488934
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 221395 ADGRF5 adhesion G protein-coupled ... NM_001098518.2 99.8% 99.8% (many diffs)
2 human 221395 ADGRF5 adhesion G protein-coupled ... NM_015234.5 99.8% 99.8% (many diffs)
3 human 221395 ADGRF5 adhesion G protein-coupled ... XM_011514353.3 98.4% 98.1% (many diffs)
4 human 221395 ADGRF5 adhesion G protein-coupled ... XM_017010419.2 98.4% 98.1% (many diffs)
5 human 221395 ADGRF5 adhesion G protein-coupled ... XM_017010420.2 98.4% 98.1% (many diffs)
6 human 221395 ADGRF5 adhesion G protein-coupled ... XM_017010421.2 98.4% 98.1% (many diffs)
7 human 221395 ADGRF5 adhesion G protein-coupled ... XM_005248892.3 96.9% 96.9% (many diffs)
8 human 221395 ADGRF5 adhesion G protein-coupled ... XM_005248893.3 96.9% 96.9% (many diffs)
9 human 221395 ADGRF5 adhesion G protein-coupled ... XM_005248894.4 95.6% 95.3% (many diffs)
10 human 221395 ADGRF5 adhesion G protein-coupled ... XM_005248895.4 95.6% 95.3% (many diffs)
11 human 221395 ADGRF5 adhesion G protein-coupled ... XM_017010418.2 95.6% 95.3% (many diffs)
12 human 221395 ADGRF5 adhesion G protein-coupled ... XM_017010422.2 92% 92% (many diffs)
13 human 221395 ADGRF5 adhesion G protein-coupled ... XM_017010423.1 86.9% 86.9% (many diffs)
14 human 221395 ADGRF5 adhesion G protein-coupled ... XM_017010424.2 85.6% 85.2% (many diffs)
15 human 221395 ADGRF5 adhesion G protein-coupled ... XM_011514354.2 84.4% 84.4% (many diffs)
16 human 112268033 LOC112268033 uncharacterized LOC112268033 XR_002956835.1 2.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
4110
ORF length:
4038
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgaaatcc ccaaggagaa ccactttgtg cctcatgttt attgtgattt
121attcttccaa agctgcactg aactggaatt acgagtctac tattcatcct ttgagtcttc
181atgaacatga accagctggt gaagaggcac tgaggcaaaa acgagccgtt gccacaaaaa
241gtcctacggc tgaagaatac actgttaata ttgagatcag ttttgaaaat gcatccttcc
301tggatcctat caaagcctac ttgaacagcc tcagttttcc aattcatggg aataacactg
361accaaattac cgacattttg agcataaatg tgacaacagt ctgcagacct gctggaaatg
421aaatctggtg ctcctgcgag acaggttatg ggtggcctcg ggaaaggtgt cttcacaatc
481tcatttgtca agagcgtgac gtcttcctcc cagggcacca ttgcagttgc cttaaagaac
541tgcctcccaa tggacctttt tgcctgcttc aggaagatgt taccctgaac atgagagtca
601gactaaatgt aggctttcaa gaagacctca tgaacacttc ctccgccctc tataggtcct
661acaagaccga cttggaaaca gcgttccgga agggttacgg aattttacca ggcttcaagg
721gcgtgactgt gacagggttc aagtctggaa gtgtggttgt gacatatgaa gtcaagacta
781caccaccatc acttgagtta atacataaag ccaatgaaca agttgtacag agcctcaatc
841agacctacaa aatggactac aactcctttc aagcagttac tatcaatgaa agcaatttct
901ttgtcacacc agaaatcatc tttgaagggg acacagtcag tctggtgtgt gaaaaggaag
961ttttgtcctc caatgtgtct tggcgctatg aagaacagca gttggaaatc cagaacagca
1021gcagattctc gatttacacc gcacttttca acaacatgac ttcggtgtcc aagctcacca
1081tccacaacat cactccaggt gatgcaggtg aatatgtttg caaactgata ttagacattt
1141ttgaatatga gtgcaagaag aaaatagatg ttatgcccat ccaaattttg gcaaatgaag
1201aaatgaaggt gatgtgcgac aacaatcctg tatctttgaa ctgctgcagt cagggtaatg
1261ttaattggag caaagtagaa tggaagcagg aaggaaaaat aaatattcca ggaacccctg
1321agacagacat agattctagc tgcagcagat acaccctcaa ggctgatgga acccagtgcc
1381caagcgggtc gtctggaaca acagtcatct acacttgtga gttcatcagt gcctatggag
1441ccagaggcag tgcaaacata aaagtgacat tcatctctgt ggccaatcta acaataaccc
1501cggacccaat ttctgtttct gagggacaaa acttttctat aaaatgcatc agtgatgtga
1561gtaactatga tgaggtttat tggaacactt ctgctggaat taaaatatac caaagatttt
1621ataccacgag gaggtatctt gatggagcag aatcagtact gacagtcaag acctcgacca
1681gggagtggaa tggaacctat cactgcatat ttagatataa gaattcatac agtattgcaa
1741ccaaagacgt cattgttcac ccgctgcctc taaagctgaa catcatggtt gatcctttgg
1801aagctactgt ttcatgcagt ggttcccatc acatcaagtg ctgcatagag gaggatggag
1861actacaaagt tactttccat atgggttcct catcccttcc tgctgcaaaa gaagttaaca
1921aaaaacaagt gtgctacaaa cacaatttca atgcaagctc agtttcctgg tgttcaaaaa
1981ctgttgatgt gtgttgtcac tttaccaatg ctgctaataa ttcagtttgg agcccatcta
2041tgaagctgaa tctggttcct ggggaaaaca tcacatgcca ggatcccgta ataggtgtcg
2101gagagccggg gaaagtcatc cagaagctat gccggttctc aaacgttccc agcagccctg
2161agagtcccat tggcgggacc atcacttaca aatgtgtagg ctcccagtgg gaggagaaga
2221gaaatgactg catctctgcc ccaataaaca gtctgctcca gatggctaag gctttgatca
2281agagcccctc tcaggatgag atgctcccta catacctgaa ggatctttct attagcatag
2341acaaagcgga acatgaaatc agctcttctc ctgggagtct gggagccatt attaacatcc
2401ttgatctgct ctcaacagtt ccaacccaag taaattcaga aatgatgacg cacgtgctct
2461ctacggttaa tgtcatcctt ggcaagcccg tcttgaacac ctggaaggtt ttacaacagc
2521aatggaccaa tcagagttca cagctactac attcagtgga aagattttcc caagcattac
2581agtcaggaga tagccctcct ttgtccttct cccaaactaa tgtgcagatg agcagcacgg
2641taatcaagtc cagccaccca gaaacctatc aacagaggtt tgttttccca tactttgacc
2701tctggggcaa tgtggtcatt gacaagagct atctagaaaa cttgcagtcg gattcgtcta
2761ttgtcaccat ggctttccca actctccaag ccatccttgc tcaggatatc caggaaaata
2821actttgcaga gagcttagtg atgacaacca ctgtcagcca caatacgact atgccattca
2881ggatttcaat gacttttaag aacaatagcc cttcaggcgg cgaaacgaag tgtgtcttct
2941ggaacttcag gcttgccaac aacacagggg ggtgggacag cagtgggtgc tatgttgaag
3001aaggtgatgg ggacaatgtc acctgtatct gtgaccacct aacatcattc tccatcctca
3061tgtcccctga ctccccagat cctagttctc tcctgggaat actcctggat attatttctt
3121atgttggggt gggcttttcc atcttgagct tggcagcctg tctagttgtg gaagctgtgg
3181tgtggaaatc ggtgaccaag aatcggactt cttatatgcg ccacacctgc atagtgaata
3241tcgctgcctc ccttctggtc gccaacacct ggttcattgt ggtcgctgcc atccaggaca
3301atcgctacat actctgcaag acagcctgtg tggctgccac cttcttcatc cacttcttct
3361acctcagcgt cttcttctgg atgctgacac tgggcctcat gctgttctat cgcctggttt
3421tcattctgca tgaaacaagc aggtccactc agaaagccat tgccttctgt cttggctatg
3481gctgcccact tgccatctcg gtcatcacgc tgggagccac ccagccccgg gaagtctata
3541cgaggaagaa tgtctgttgg ctcaactggg aggacaccaa ggccctgctg gctttcgcca
3601tcccagcact gatcattgtg gtggtgaaca taaccatcac tattgtggtc atcaccaaga
3661tcctgaggcc ttccattgga gacaagccat gcaagcagga gaagagcagc ctgtttcaga
3721tcagcaagag cattggggtc ctcacaccac tcttgggcct cacttGGGGT TTTGGTCTCA
3781CCACTGTGTT CCCAGGGACC AACCTTGTGT TCCATATCAT ATTTGCCATC CTCAATGTCT
3841TCCAGGGATT ATTCATTTTA CTCTTTGGAT GCCTCTGGGA TCTGAAGGTA CAGGAAGCTT
3901TGCTGAATAA GTTTTCATTG TCGAGATGGT CTTCACAGCA CTCAAAGTCA ACATCCCTGG
3961GTTCATCCAC ACCTGTGTTT TCTATGAGTT CTCCAATATC AAGGAGATTT AACAATTTGT
4021TTGGTAAAAC AGGAACGTAT AATGTTTCCA CCCCAGAAGC AACCAGCTCA TCCCTGGAAA
4081ACTCATCCAG TGCTTCTTCG TTGCTCAACT AAGACCCAGC TTTCTTGTAC AAAGTGGTTG
4141ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
4201GTCCGTAACT TGAAAGTATT TCGATTTTTG GCTTTATATA TCTTGTGGAA AGGACGAATC
4261CCTTTCGACC TCTAAACCAT CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt
4321tgtgaaagat t

Download FASTA (ORF) (Full)

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