Construct: ORF TRCN0000488934
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021134.1_s317c1
- DNA Barcode:
- ATCCCTTTCGACCTCTAAACCATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ADGRF5 (221395)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488934
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | NM_001098518.2 | 99.8% | 99.8% | (many diffs) |
| 2 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | NM_015234.5 | 99.8% | 99.8% | (many diffs) |
| 3 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | XM_011514353.3 | 98.4% | 98.1% | (many diffs) |
| 4 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | XM_017010419.2 | 98.4% | 98.1% | (many diffs) |
| 5 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | XM_017010420.2 | 98.4% | 98.1% | (many diffs) |
| 6 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | XM_017010421.2 | 98.4% | 98.1% | (many diffs) |
| 7 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | XM_005248892.3 | 96.9% | 96.9% | (many diffs) |
| 8 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | XM_005248893.3 | 96.9% | 96.9% | (many diffs) |
| 9 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | XM_005248894.4 | 95.6% | 95.3% | (many diffs) |
| 10 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | XM_005248895.4 | 95.6% | 95.3% | (many diffs) |
| 11 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | XM_017010418.2 | 95.6% | 95.3% | (many diffs) |
| 12 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | XM_017010422.2 | 92% | 92% | (many diffs) |
| 13 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | XM_017010423.1 | 86.9% | 86.9% | (many diffs) |
| 14 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | XM_017010424.2 | 85.6% | 85.2% | (many diffs) |
| 15 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | XM_011514354.2 | 84.4% | 84.4% | (many diffs) |
| 16 | human | 112268033 | LOC112268033 | uncharacterized LOC112268033 | XR_002956835.1 | 2.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 4110
- ORF length:
- 4038
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaaatcc ccaaggagaa ccactttgtg cctcatgttt attgtgattt 121 attcttccaa agctgcactg aactggaatt acgagtctac tattcatcct ttgagtcttc 181 atgaacatga accagctggt gaagaggcac tgaggcaaaa acgagccgtt gccacaaaaa 241 gtcctacggc tgaagaatac actgttaata ttgagatcag ttttgaaaat gcatccttcc 301 tggatcctat caaagcctac ttgaacagcc tcagttttcc aattcatggg aataacactg 361 accaaattac cgacattttg agcataaatg tgacaacagt ctgcagacct gctggaaatg 421 aaatctggtg ctcctgcgag acaggttatg ggtggcctcg ggaaaggtgt cttcacaatc 481 tcatttgtca agagcgtgac gtcttcctcc cagggcacca ttgcagttgc cttaaagaac 541 tgcctcccaa tggacctttt tgcctgcttc aggaagatgt taccctgaac atgagagtca 601 gactaaatgt aggctttcaa gaagacctca tgaacacttc ctccgccctc tataggtcct 661 acaagaccga cttggaaaca gcgttccgga agggttacgg aattttacca ggcttcaagg 721 gcgtgactgt gacagggttc aagtctggaa gtgtggttgt gacatatgaa gtcaagacta 781 caccaccatc acttgagtta atacataaag ccaatgaaca agttgtacag agcctcaatc 841 agacctacaa aatggactac aactcctttc aagcagttac tatcaatgaa agcaatttct 901 ttgtcacacc agaaatcatc tttgaagggg acacagtcag tctggtgtgt gaaaaggaag 961 ttttgtcctc caatgtgtct tggcgctatg aagaacagca gttggaaatc cagaacagca 1021 gcagattctc gatttacacc gcacttttca acaacatgac ttcggtgtcc aagctcacca 1081 tccacaacat cactccaggt gatgcaggtg aatatgtttg caaactgata ttagacattt 1141 ttgaatatga gtgcaagaag aaaatagatg ttatgcccat ccaaattttg gcaaatgaag 1201 aaatgaaggt gatgtgcgac aacaatcctg tatctttgaa ctgctgcagt cagggtaatg 1261 ttaattggag caaagtagaa tggaagcagg aaggaaaaat aaatattcca ggaacccctg 1321 agacagacat agattctagc tgcagcagat acaccctcaa ggctgatgga acccagtgcc 1381 caagcgggtc gtctggaaca acagtcatct acacttgtga gttcatcagt gcctatggag 1441 ccagaggcag tgcaaacata aaagtgacat tcatctctgt ggccaatcta acaataaccc 1501 cggacccaat ttctgtttct gagggacaaa acttttctat aaaatgcatc agtgatgtga 1561 gtaactatga tgaggtttat tggaacactt ctgctggaat taaaatatac caaagatttt 1621 ataccacgag gaggtatctt gatggagcag aatcagtact gacagtcaag acctcgacca 1681 gggagtggaa tggaacctat cactgcatat ttagatataa gaattcatac agtattgcaa 1741 ccaaagacgt cattgttcac ccgctgcctc taaagctgaa catcatggtt gatcctttgg 1801 aagctactgt ttcatgcagt ggttcccatc acatcaagtg ctgcatagag gaggatggag 1861 actacaaagt tactttccat atgggttcct catcccttcc tgctgcaaaa gaagttaaca 1921 aaaaacaagt gtgctacaaa cacaatttca atgcaagctc agtttcctgg tgttcaaaaa 1981 ctgttgatgt gtgttgtcac tttaccaatg ctgctaataa ttcagtttgg agcccatcta 2041 tgaagctgaa tctggttcct ggggaaaaca tcacatgcca ggatcccgta ataggtgtcg 2101 gagagccggg gaaagtcatc cagaagctat gccggttctc aaacgttccc agcagccctg 2161 agagtcccat tggcgggacc atcacttaca aatgtgtagg ctcccagtgg gaggagaaga 2221 gaaatgactg catctctgcc ccaataaaca gtctgctcca gatggctaag gctttgatca 2281 agagcccctc tcaggatgag atgctcccta catacctgaa ggatctttct attagcatag 2341 acaaagcgga acatgaaatc agctcttctc ctgggagtct gggagccatt attaacatcc 2401 ttgatctgct ctcaacagtt ccaacccaag taaattcaga aatgatgacg cacgtgctct 2461 ctacggttaa tgtcatcctt ggcaagcccg tcttgaacac ctggaaggtt ttacaacagc 2521 aatggaccaa tcagagttca cagctactac attcagtgga aagattttcc caagcattac 2581 agtcaggaga tagccctcct ttgtccttct cccaaactaa tgtgcagatg agcagcacgg 2641 taatcaagtc cagccaccca gaaacctatc aacagaggtt tgttttccca tactttgacc 2701 tctggggcaa tgtggtcatt gacaagagct atctagaaaa cttgcagtcg gattcgtcta 2761 ttgtcaccat ggctttccca actctccaag ccatccttgc tcaggatatc caggaaaata 2821 actttgcaga gagcttagtg atgacaacca ctgtcagcca caatacgact atgccattca 2881 ggatttcaat gacttttaag aacaatagcc cttcaggcgg cgaaacgaag tgtgtcttct 2941 ggaacttcag gcttgccaac aacacagggg ggtgggacag cagtgggtgc tatgttgaag 3001 aaggtgatgg ggacaatgtc acctgtatct gtgaccacct aacatcattc tccatcctca 3061 tgtcccctga ctccccagat cctagttctc tcctgggaat actcctggat attatttctt 3121 atgttggggt gggcttttcc atcttgagct tggcagcctg tctagttgtg gaagctgtgg 3181 tgtggaaatc ggtgaccaag aatcggactt cttatatgcg ccacacctgc atagtgaata 3241 tcgctgcctc ccttctggtc gccaacacct ggttcattgt ggtcgctgcc atccaggaca 3301 atcgctacat actctgcaag acagcctgtg tggctgccac cttcttcatc cacttcttct 3361 acctcagcgt cttcttctgg atgctgacac tgggcctcat gctgttctat cgcctggttt 3421 tcattctgca tgaaacaagc aggtccactc agaaagccat tgccttctgt cttggctatg 3481 gctgcccact tgccatctcg gtcatcacgc tgggagccac ccagccccgg gaagtctata 3541 cgaggaagaa tgtctgttgg ctcaactggg aggacaccaa ggccctgctg gctttcgcca 3601 tcccagcact gatcattgtg gtggtgaaca taaccatcac tattgtggtc atcaccaaga 3661 tcctgaggcc ttccattgga gacaagccat gcaagcagga gaagagcagc ctgtttcaga 3721 tcagcaagag cattggggtc ctcacaccac tcttgggcct cacttGGGGT TTTGGTCTCA 3781 CCACTGTGTT CCCAGGGACC AACCTTGTGT TCCATATCAT ATTTGCCATC CTCAATGTCT 3841 TCCAGGGATT ATTCATTTTA CTCTTTGGAT GCCTCTGGGA TCTGAAGGTA CAGGAAGCTT 3901 TGCTGAATAA GTTTTCATTG TCGAGATGGT CTTCACAGCA CTCAAAGTCA ACATCCCTGG 3961 GTTCATCCAC ACCTGTGTTT TCTATGAGTT CTCCAATATC AAGGAGATTT AACAATTTGT 4021 TTGGTAAAAC AGGAACGTAT AATGTTTCCA CCCCAGAAGC AACCAGCTCA TCCCTGGAAA 4081 ACTCATCCAG TGCTTCTTCG TTGCTCAACT AAGACCCAGC TTTCTTGTAC AAAGTGGTTG 4141 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 4201 GTCCGTAACT TGAAAGTATT TCGATTTTTG GCTTTATATA TCTTGTGGAA AGGACGAATC 4261 CCTTTCGACC TCTAAACCAT CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt 4321 tgtgaaagat t