Construct: ORF TRCN0000488937
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020903.1_s317c1
- DNA Barcode:
- CCTCGACCCATGGTACCCCGTTTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- LPAR6 (10161)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488937
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10161 | LPAR6 | lysophosphatidic acid recep... | NM_001162497.2 | 100% | 100% | |
| 2 | human | 10161 | LPAR6 | lysophosphatidic acid recep... | NM_001162498.2 | 100% | 100% | |
| 3 | human | 10161 | LPAR6 | lysophosphatidic acid recep... | NM_005767.6 | 100% | 100% | |
| 4 | human | 10161 | LPAR6 | lysophosphatidic acid recep... | XM_024449302.1 | 100% | 100% | |
| 5 | mouse | 67168 | Lpar6 | lysophosphatidic acid recep... | NM_175116.4 | 87.4% | 93% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1104
- ORF length:
- 1032
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggtaagc gttaacagct cccactgctt ctataatgac tcctttaagt 121 acactttgta tgggtgcatg ttcagcatgg tgtttgtgct tgggttaata tccaattgtg 181 ttgccatata cattttcatc tgcgtcctca aagtccgaaa tgaaactaca acttacatga 241 ttaacttggc aatgtcagac ttgctttttg tttttacttt acccttcagg attttttact 301 tcacaacacg gaattggcca tttggagatt tactttgtaa gatttctgtg atgctgtttt 361 ataccaacat gtacggaagc attctgttct taacctgtat tagtgtagat cgatttctgg 421 caattgtcta cccatttaag tcaaagactc taagaaccaa aagaaatgca aagattgttt 481 gcactggcgt gtggttaact gtgatcggag gaagtgcacc cgccgttttt gttcagtcta 541 cccactctca gggtaacaat gcctcagaag cctgctttga aaattttcca gaagccacat 601 ggaaaacata tctctcaagg attgtaattt tcatcgaaat agtgggattt tttattcctc 661 taattttaaa tgtaacttgt tctagtatgg tgctaaaaac tttaaccaaa cctgttacat 721 taagtagaag caaaataaac aaaactaagg ttttaaaaat gatttttgta catttgatca 781 tattctgttT CTGTTTTGTT CCTTACAATA TCAATCTTAT TTTATATTCT CTTGTGAGAA 841 CACAAACATT TGTTAATTGC TCAGTAGTGG CAGCAGTAAG GACAATGTAC CCAATCACTC 901 TCTGTATTGC TGTTTCCAAC TGTTGTTTTG ACCCTATAGT TTACTACTTT ACATCGGACA 961 CAATTCAGAA TTCAATAAAA ATGAAAAACT GGTCTGTCAG GAGAAGTGAC TTCAGATTCT 1021 CTGAAGTTCA TGGTGCAGAG AATTTTATTC AGCATAACCT ACAGACCTTA AAAAGTAAGA 1081 TATTTGACAA TGAATCTGCT GCCTAGGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1141 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1201 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACCTCGACC 1261 CATGGTACCC CGTTTCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1321 aagatt