Construct: ORF TRCN0000488945
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020149.1_s317c1
- DNA Barcode:
- ACTCTAAGTAGTCCTCTAATTCAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RIOK2 (55781)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488945
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55781 | RIOK2 | RIO kinase 2 | NM_018343.3 | 99.9% | 99.8% | 430C>T |
| 2 | human | 55781 | RIOK2 | RIO kinase 2 | NM_001159749.2 | 85.3% | 84.6% | (many diffs) |
| 3 | human | 55781 | RIOK2 | RIO kinase 2 | XM_017009628.1 | 66.1% | 66.1% | 0_1ins561 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1728
- ORF length:
- 1656
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggggaaa gtgaatgtgg ccaagttgcg ttacatgagc cgagatgact 121 tcagggtctt gaccgcggtt gaaatgggca tgaagaacca tgaaattgtt cccggcagtt 181 tgattgcttc tatagccagc cttaaacatg gtggctgtaa taaagtttta agagaattag 241 tgaaacataa actcatagct tgggagcgta ccaaaactgt ccagggctat cggttgacaa 301 atgcaggata tgattaccta gctttgaaaa cactttcttc taggcaagta gttgagtctg 361 ttggaaacca gatgggtgtt ggcaaagaat cagatattta cattgttgca aatgaagaag 421 gacaacaatt tgcattaaag cttcacagac taggaagaac ctcgtttcga aatttgaaaa 481 acaaacgcga ttatcataaa tataggcaca atgtgtcatg gctatattta tctcgtctct 541 ctgccatgaa ggaatttgcc tatatgaagg cattgtatga gaggaaattt ccagttccaa 601 agccaattga ttacaatcgt catgcagtgg tcatggaact cataaatggt tatccactat 661 gtcagataca ccatgttgaa gatcctgcat cagtatatga tgaagctatg gaactaattg 721 tcaaacttgc aaatcatggg ctgattcatg gagattttaa tgaatttaat ctcattttgg 781 atgaaagtga ccatatcacc atgattgatt ttccacagat ggtttcaact tctcatccca 841 atgctgagtg gtattttgac agagatgtta aatgcattaa agatttcttt atgaaacgtt 901 tcagctacga aagtgagctt tttccaactt ttaaggatat caggagagaa gacactcttg 961 atgtggaggt ttctgccagt ggctacacaa aggaaatgca ggcagatgat gaactgcttc 1021 atccattagg tccagatgat aaaaatattg aaacaaaaga gggatctgaa ttctcatttt 1081 cagatggaga agtggcagaa aaagcagagg tttacgggtc agaaaatgaa agtgaacgga 1141 actgtctaga agaatcagag ggctgctatt gcagatcatc tggagaccct gaacaaataa 1201 aggaagacag tttatcagaa gagagtgctg atgcacggag ttttgaaatg actgaattca 1261 atcaagcttt agaagaaata aaagggcagg ttgttgaaaa caactctgta actgaatttt 1321 ctgaggagaa aaacagaact gaaaattaca acaggcaaga tggtcagaga gttcaaggag 1381 gagtccctgc tggctctgac gagtatgaag atgaatgccc tcatctaatt gccttgtcgt 1441 cattaaatag agaattcagg CCTTTCAGAG ATGAAGAAAA TGTGGGAGCT ATGAATCAGT 1501 ATAGAACAAG AACTCTGAGT ATCACTTCTT CAGGCAGTGC TGTAAGCTGT TCAACAATTC 1561 CTCCAGAACT GGTGAAACAG AAGGTGAAAC GTCAGTTGAC AAAACAGCAA AAATCAGCTG 1621 TCAGACGTCG ATTGCAGAAA GGAGAAGCAA ATATATTTAC CAAGCAACGT AGGGAAAACA 1681 TGCAAAATAT CAAATCAAGT TTGGAAGCAG CCAGCTTTTG GGGAGAATGA GACCCAGCTT 1741 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1801 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1861 CTTGTGGAAA GGACGAACTC TAAGTAGTCC TCTAATTCAA ACGCGTTAAG TCgacaatca 1921 acctctggat tacaaaattt gtgaaagatt