Construct: ORF TRCN0000488974
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020332.1_s317c1
- DNA Barcode:
- ATCGCGTACCGCGCCTTATAACAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CHEK1 (1111)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488974
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1111 | CHEK1 | checkpoint kinase 1 | NM_001114121.2 | 99.9% | 99.7% | 1411A>G |
| 2 | human | 1111 | CHEK1 | checkpoint kinase 1 | NM_001114122.2 | 99.9% | 99.7% | 1411A>G |
| 3 | human | 1111 | CHEK1 | checkpoint kinase 1 | NM_001274.5 | 99.9% | 99.7% | 1411A>G |
| 4 | human | 1111 | CHEK1 | checkpoint kinase 1 | XM_017017146.2 | 99.9% | 99.7% | 1411A>G |
| 5 | human | 1111 | CHEK1 | checkpoint kinase 1 | XM_024448337.1 | 99.9% | 99.7% | 1411A>G |
| 6 | human | 1111 | CHEK1 | checkpoint kinase 1 | NM_001244846.1 | 92.7% | 92.6% | 1231_1232ins102;1309A>G |
| 7 | human | 1111 | CHEK1 | checkpoint kinase 1 | NM_001330428.1 | 80% | 79.4% | (many diffs) |
| 8 | human | 1111 | CHEK1 | checkpoint kinase 1 | NM_001330427.1 | 70.7% | 76.1% | 1_272del;337_338ins224;1459A>G |
| 9 | human | 1111 | CHEK1 | checkpoint kinase 1 | XM_011542560.2 | 70.7% | 76.1% | 1_272del;337_338ins224;1459A>G |
| 10 | human | 1111 | CHEK1 | checkpoint kinase 1 | NR_045205.1 | 38.4% | 1_434del;1845A>G;1863_3711del | |
| 11 | human | 1111 | CHEK1 | checkpoint kinase 1 | NR_045204.1 | 33.1% | (many diffs) | |
| 12 | mouse | 12649 | Chek1 | checkpoint kinase 1 | NM_007691.5 | 89% | 93.2% | (many diffs) |
| 13 | mouse | 12649 | Chek1 | checkpoint kinase 1 | XM_017313117.1 | 69.8% | 72.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1500
- ORF length:
- 1428
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcagtg ccctttgtgg aagactggga cttggtgcaa accctgggag 121 aaggtgccta tggagaagtt caacttgctg tgaatagagt aactgaagaa gcagtcgcag 181 tgaagattgt agatatgaag cgtgccgtag actgtccaga aaatattaag aaagagatct 241 gtatcaataa aatgctaaat catgaaaatg tagtaaaatt ctatggtcac aggagagaag 301 gcaatatcca atatttattt ctggagtact gtagtggagg agagcttttt gacagaatag 361 agccagacat aggcatgcct gaaccagatg ctcagagatt cttccatcaa ctcatggcag 421 gggtggttta tctgcatggt attggaataa ctcacaggga tattaaacca gaaaatcttc 481 tgttggatga aagggataac ctcaaaatct cagactttgg cttggcaaca gtatttcggt 541 ataataatcg tgagcgtttg ttgaacaaga tgtgtggtac tttaccatat gttgctccag 601 aacttctgaa gagaagagaa tttcatgcag aaccagttga tgtttggtcc tgtggaatag 661 tacttactgc aatgctcgct ggagaattgc catgggacca acccagtgac agctgtcagg 721 agtattctga ctggaaagaa aaaaaaacat acctcaaccc ttggaaaaaa atcgattctg 781 ctcctctagc tctgctgcat aaaatcttag ttgagaatcc atcagcaaga attaccattc 841 cagacatcaa aaaagataga tggtacaaca aacccctcaa gaaaggggca aaaaggcccc 901 gagtcacttc aggtggtgtg tcagagtctc ccagtggatt ttctaagcac attcaatcca 961 atttggactt ctctccagta aacagtgctt ctagtgaaga aaatgtgaag tactccagtt 1021 ctcagccaga accccgcaca ggtctttcct tatgggatac cagcccctca tacattgata 1081 aattggtaca agggatcagc ttttcccagc ccacatgtcc tgatcatatg cttttgaata 1141 gtcagttact tggcacccca ggatcctcac agaacccctg gcagcggttg gtcaaaagaa 1201 tgacacgatt ctttaccaaa ttGGATGCAG ACAAATCTTA TCAATGCCTG AAAGAGACTT 1261 GTGAGAAGTT GGGCTATCAA TGGAAGAAAA GTTGTATGAA TCAGGTTACT ATATCAACAA 1321 CTGATAGGAG AAACAATAAA CTCATTTTCA AAGTGAATTT GTTAGAAATG GATGATAAAA 1381 TATTGGTTGA CTTCCGGCTT TCTAAGGGTG ATGGATTGGA GTTCAAGAGA CACTTCCTGA 1441 AGATTAAAGG GAAGCTGATT GATATTGTGA GCAGCCAGAA GGTTTGGCTT CCTGCCACAT 1501 GAGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1561 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1621 GGCTTTATAT ATCTTGTGGA AAGGACGAAT CGCGTACCGC GCCTTATAAC ATACGCGTTA 1681 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt