Construct: ORF TRCN0000488975
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020160.1_s317c1
- DNA Barcode:
- TAATCCACATTTACTCCCAACTTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PBK (55872)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488975
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55872 | PBK | PDZ binding kinase | NM_001363040.2 | 99.7% | 99.6% | 320A>G;822G>C |
| 2 | human | 55872 | PBK | PDZ binding kinase | NM_018492.4 | 99.7% | 99.6% | 320A>G;822G>C |
| 3 | human | 55872 | PBK | PDZ binding kinase | NM_001278945.2 | 96.4% | 96.3% | 320A>G;595_627del;855G>C |
| 4 | mouse | 52033 | Pbk | PDZ binding kinase | NM_023209.2 | 84.7% | 85.8% | (many diffs) |
| 5 | mouse | 52033 | Pbk | PDZ binding kinase | XM_006519257.3 | 84.7% | 85.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1038
- ORF length:
- 966
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaaggg atcagtaatt tcaagacacc aagcaaatta tcagaaaaaa 121 agaaatctgt attatgttca actccaacta taaatatccc ggcctctccg tttatgcaga 181 agcttggctt tggtactggg gtaaatgtgt acctaatgaa aagatctcca agaggtttgt 241 ctcattctcc ttgggctgta aaaaagatta atcctatatg taatgatcat tatcgaagtg 301 tgtatcaaaa gagactaatg gatgaagcta agattttgaa aagccttcat catccaaaca 361 ttgttggtta tcgtgctttt actgaagcca gtgatggcag tctgtgtctt gctatggaat 421 atggaggtga aaagtctcta aatgacttaa tagaagaacg atataaagcc agccaagatc 481 cttttccagc agccataatt ttaaaagttg ctttgaatat ggcaagaggg ttaaagtatc 541 tgcaccaaga aaagaaactg cttcatggag acataaagtc ttcaaatgtt gtaattaaag 601 gcgattttga aacaattaaa atctgtgatg taggagtctc tctaccactg gatgaaaata 661 tgactgtgac tgaccctGAG GCTTGTTACA TTGGCACAGA GCCATGGAAA CCCAAAGAAG 721 CTGTGGAGGA GAATGGTGTT ATTACTGACA AGGCAGACAT ATTTGCCTTT GGCCTTACTT 781 TGTGGGAAAT GATGACTTTA TCGATTCCAC ACATTAATCT TTCAAATGAT GATGATGATG 841 AAGATAAAAC TTTTGATGAA AGTGATTTTG ATGATGAAGC ATACTATGCA GCCTTGGGAA 901 CTAGGCCACC TATTAATATG GAAGAACTGG ATGAATCATA CCAGAAAGTA ATTGAACTCT 961 TCTCTGTATG CACTAATGAA GACCCTAAAG ATCGTCCTTC TGCTGCACAC ATTGTTGAAG 1021 CTCTGGAAAC AGATGTCTGA GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1081 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1141 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATAAT CCACATTTAC 1201 TCCCAACTTA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt