Construct: ORF TRCN0000488981
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020047.1_s317c1
- DNA Barcode:
- ACCATGCCGATACTTCCCGTTTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR50 (9248)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488981
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9248 | GPR50 | G protein-coupled receptor 50 | NM_004224.3 | 99.8% | 99.6% | 1816A>G;1851_1852insG |
| 2 | human | 9248 | GPR50 | G protein-coupled receptor 50 | XM_011531216.2 | 59.8% | 59.5% | 1_1delAins742;1075A>G;1110_1111insG |
| 3 | mouse | 14765 | Gpr50 | G-protein-coupled receptor 50 | NM_001308501.1 | 32.6% | 33.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1923
- ORF length:
- 1854
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggggcccacc ctagcggttc ccacccccta tggctgtatt ggctgtaagc 121 taccccagcc agaataccca ccggctctaa tcatctttat gttctgcgcg atggttatca 181 ccatcgttgt agacctaatc ggcaactcca tggtcatttt ggctgtgacg aagaacaaga 241 agctccggaa ttctggcaac atcttcgtgg tcagtctctc tgtggccgat atgctggtgg 301 ccatctaccc ataccctttg atgctgcatg ccatgtccat tgggggctgg gatctgagcc 361 agttacagtg ccagatggtc gggttcatca cagggctgag tgtggtcggc tccatcttca 421 acatcgtggc aatcgctatc aaccgttact gctacatctg ccacagcctc cagtacgaac 481 ggatcttcag tgtgcgcaat acctgcatct acctggtcat cacctggatc atgaccgtcc 541 tggctgtcct gcccaacatg tacattggca ccatcgagta cgatcctcgc acctacacct 601 gcatcttcaa ctatctgaac aaccctgtct tcactgttac catcgtctgc atccacttcg 661 tcctccctct cctcatcgtg ggtttctgct acgtgaggat ctggaccaaa gtgctggcgg 721 cccgtgaccc tgcagggcag aatcctgaca accaacttgc tgaggttcgc aattttctaa 781 ccatgtttgt gatcttcctc ctctttgcag tgtgctggtg ccctatcaac gtgctcactg 841 tcttggtggc tgtcagtccg aaggagatgg caggcaagat ccccaactgg ctttatcttg 901 cagcctactt catagcctac ttcaacagct gcctcaacgc tgtgatctac gggctcctca 961 atgagaattt ccgaagagaa tactggacca tcttccatgc tatgcggcac cctatcatat 1021 tcttctctgg cctcatcagt gatattcgtg agatgcagga ggcccgtacc ctggcccgcg 1081 cccgtgccca tgctcgcgac caagctcgtg aacaagaccg tgcccatgcc tgtcctgctg 1141 tggaggaaac cccgatgaat gtccggaatg ttccattacc tggtgatgct gcagctggcc 1201 accccgaccg tgcctctggc caccctaagc cccattccag atcctcctct gcctatcgca 1261 aatctgcctc tacccaccac aagtctgtct ttagccactc caaggctgcc tctggtcacc 1321 tcaagcctgt ctctggccac tccaagcctg cctctggtca ccccaagtct gccactgtct 1381 accctaagcc tgcctctgtc catttcaagg ctgactctgt ccatttcaag ggtgactctg 1441 tccatttcaa gcctgactct gttcatttca agcctgcttc cagcaacccc aagcccatca 1501 ctggccacca tgtctctgct ggcagccact ccaagtctgc cttcagtgct gccaccagcc 1561 accctaaacc caccactggc cacatcaagc cagctaccag ccatgctgag cccaccactg 1621 ctgactatcc caagcctgcc actaccagcc accctaagcc cactgctgct gacaacccTG 1681 AGCTCTCTGC CTCCCATTGC CCCGAGATCC CTGCCATTGC CCACCCTGTG TCTGACGACA 1741 GTGACCTCCC TGAGTCGGCC TCTAGCCCTG CCGCTGGGCC CACCAAGCCT GCTGCCAGCC 1801 AGCTGGAGTC TGACACCATC GCTGACCTTC CTGACCCTAC TGTAGTCACT ACCAGTACCA 1861 ATGATTACCA TGATGTCGTG GTTGTTGATG TTGAAGATGA TCCTGATGAA ATGGCTGTGG 1921 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1981 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2041 TTTATATATC TTGTGGAAAG GACGAACCAT GCCGATACTT CCCGTTTAGA CGCGTTAAGT 2101 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt