Construct: ORF TRCN0000488982
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019941.1_s317c1
- DNA Barcode:
- GGATGGAGTGCCCCATCCATTGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GLP2R (9340)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488982
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9340 | GLP2R | glucagon like peptide 2 rec... | NM_004246.3 | 99.9% | 99.8% | 1659_1660insG |
| 2 | human | 9340 | GLP2R | glucagon like peptide 2 rec... | XM_017025339.1 | 98.8% | 98.7% | 381_398del;1677_1678insG |
| 3 | human | 9340 | GLP2R | glucagon like peptide 2 rec... | XM_011524077.3 | 95.6% | 95.5% | 380_454del;1734_1735insG |
| 4 | human | 9340 | GLP2R | glucagon like peptide 2 rec... | XM_017025340.1 | 87.2% | 82.3% | 189_190ins88;294_295ins122;1449_1450insG |
| 5 | human | 9340 | GLP2R | glucagon like peptide 2 rec... | XM_017025341.1 | 80.9% | 80.8% | 189_190ins315;1344_1345insG |
| 6 | human | 9340 | GLP2R | glucagon like peptide 2 rec... | XM_005256861.3 | 72.2% | 69.6% | 0_1ins337;45_46ins122;1200_1201insG |
| 7 | mouse | 93896 | Glp2r | glucagon-like peptide 2 rec... | NM_175681.3 | 77.4% | 74.5% | (many diffs) |
| 8 | mouse | 93896 | Glp2r | glucagon-like peptide 2 rec... | XM_006534561.3 | 76% | 73.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1734
- ORF length:
- 1662
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaagctg ggatcgagca gggcagggcc tgggagagga agcgcgggac 121 tcctgcctgg cgtccacgag ctgcccatgg gcatccctgc cccctggggg accagtcctc 181 tctccttcca caggaagtgc tctctctggg cccctgggag gcccttcctc actctggtcc 241 tgctggtttc catcaagcaa gttacaggat ccctccttga ggaaacgact cggaagtggg 301 ctcagtacaa acaggcatgt ctgagagact tactcaagga accttctggc atattttgta 361 acgggacatt tgatcagtac gtgtgttggc ctcattcttc tcctggaaat gtctctgtac 421 cctgcccttc atacttacct tggtggagtg aagagagctc aggaagggcc tacagacact 481 gcttggctca ggggacttgg cagacgatag agaacgccac ggatatttgg caggatgact 541 ccgaatgctc cgagaaccac agcttcaagc aaaacgtgga tcgttatgcc ttgctgtcaa 601 ccttgcagct gatgtacacc gtgggatact ccttctctct tatctccctc ttcctggctc 661 tcaccctcct cttgtttctt cgaaaactcc actgcacgcg caactacatc cacatgaact 721 tgtttgcttc tttcatcctg agaaccctgg ctgtactggt gaaggacgtc gtcttctaca 781 actcttactc caagaggcct gacaatgaga atgggtggat gtcctacctg tcagagatgt 841 ccacctcctg ccgctcagtc caggttctct tgcattactt tgtgggtgcc aattacttat 901 ggctgctggt tgaaggcctc tacctccaca cgctgctgga gcccacagtg cttcctgaga 961 ggcggctgtg gcccagatac ctgctgttgg gttgggcctt ccctgtgcta tttgttgtac 1021 cctggggttt cgcccgtgca cacctggaga acacagggtg ctggacaaca aatgggaata 1081 agaaaatctg gtggatcatc cgaggaccca tgatgctctg tgtaacagtc aatttcttca 1141 tcttcctgaa aattctcaag cttctcattt ctaagctcaa agctcatcaa atgtgcttca 1201 gagattataa atacagattg gcaaaatcaa cactggtcct cattccttta ttgggcgttc 1261 atgagatcct cttctctttc atcactgatg atcaagttga aggatttgca aaacttatac 1321 gacttttcat tcagttgaca ctgagctcct ttcatgggtt cctggtggcc ttgcagtatg 1381 gttttgccaa tggagaggtg aaggctgagc tgcggaaata ctgggtccgc ttcttgctag 1441 cccgccacTC AGGCTGCAGA GCCTGTGTCC TGGGGAAGGA CTTCCGGTTC CTAGGAAAAT 1501 GTCCCAAGAA GCTCTCGGAA GGAGATGGCG CTGAGAAGCT TCGGAAGCTG CAGCCCTCAC 1561 TTAACAGTGG GCGGCTCCTA CATCTAGCCA TGCGAGGTCT TGGGGAGCTG GGCGCCCAGC 1621 CCCAACAGGA CCATGCACGC TGGCCCCGGG GCAGCAGCCT GTCCGAGTGC AGTGAGGGGG 1681 ATGTCACCAT GGCCAACACC ATGGAGGAGA TTCTGGAAGA GAGTGAGATC GACCCAGCTT 1741 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1801 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1861 CTTGTGGAAA GGACGAGGAT GGAGTGCCCC ATCCATTGGT ACGCGTTAAG TCgacaatca 1921 acctctggat tacaaaattt gtgaaagatt