Construct: ORF TRCN0000489007
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020200.1_s317c1
- DNA Barcode:
- AAGCCGATGGAAGGTTAATACAGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- DYRK2 (8445)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489007
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8445 | DYRK2 | dual specificity tyrosine p... | NM_006482.3 | 100% | 100% | |
| 2 | human | 8445 | DYRK2 | dual specificity tyrosine p... | NM_003583.4 | 87.8% | 87.8% | 0_1ins219 |
| 3 | human | 8445 | DYRK2 | dual specificity tyrosine p... | XM_017020032.1 | 87.8% | 87.8% | 0_1ins219 |
| 4 | mouse | 69181 | Dyrk2 | dual-specificity tyrosine-(... | NM_001014390.2 | 90.4% | 97.1% | (many diffs) |
| 5 | mouse | 69181 | Dyrk2 | dual-specificity tyrosine-(... | XM_006514046.3 | 80.4% | 86.2% | (many diffs) |
| 6 | mouse | 69181 | Dyrk2 | dual-specificity tyrosine-(... | XM_011243552.2 | 79.1% | 86% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1875
- ORF length:
- 1803
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgttaacc aggaaacctt cggccgccgc tcccgccgcc tacccgaccg 121 gccgaggtgg ggacagcgcc gttcgtcagc ttcaggcttc cccggggctc ggtgcagggg 181 ccacccggag cggagtgggg actggcccgc cctcccccat cgccctgccg cctctccggg 241 ccagcaacgc tgccgccgca gcccacacga ttggcggcag taagcacaca atgaatgatc 301 acctgcatgt cggcagccac gctcacggac agatccaggt tcaacagttg tttgaggata 361 acagtaacaa gcggacagtg ctcacgacac aaccaaatgg gcttacaaca gtgggcaaaa 421 cgggcttgcc agtggtgcca gagcggcagc tggacagcat tcatagacgg caggggagct 481 ccacctctct aaagtccatg gaaggcatgg ggaaggtgaa agccaccccc atgacacctg 541 aacaagcaat gaagcaatac atgcaaaaac tcacagcctt cgaacaccat gagattttca 601 gctaccctga aatatatttc ttgggtctaa atgctaagaa gcgccagggc atgacaggtg 661 ggcccaacaa tggtggctat gatgatgacc agggatcata tgtgcaggtg ccccacgatc 721 acgtggctta caggtatgag gtcctcaagg tcattgggaa ggggagcttt gggcaggtgg 781 tcaaggccta cgatcacaaa gtccaccagc acgtggccct aaagatggtg cggaatgaga 841 agcgcttcca ccggcaagca gcggaggaga tccgaatcct ggaacacctg cggaagcagg 901 acaaggataa cacaatgaat gtcatccata tgctggagaa tttcaccttc cgcaaccaca 961 tctgcatgac gtttgagctg ctgagcatga acctctatga gctcatcaag aagaataaat 1021 tccagggctt cagtctgcct ttggttcgca agtttgccca ctcgattctg cagtgcttgg 1081 atgctttgca caaaaacaga ataattcact gtgaccttaa gcccgagaac attttgttaa 1141 agcagcaggg tagaagcggt attaaagtaa ttgattttgg ctccagttgt tacgagcatc 1201 agcgtgtcta cacgtacatc cagtcgcgtt tttaccgggc tccagaagtg atccttgggg 1261 ccaggtatgg catgcccatt gatatgtgga gcctgggctg cattttagca gagctcctga 1321 cgggttaccc cctcttgcct ggggaagatg aaggggacca gctggcctgt atgattgaac 1381 tgttgggcat gccctcacag aaactgctgg atgcatccaa acgagccaaa aattttgtga 1441 gctccaaggg ttatccccgt tactgcactg tcacgactct ctcagatggc tctgtggtcc 1501 TAAACGGAGG CCGTTCCCGG AGGGGGAAAC TGAGGGGCCC ACCGGAGAGC AGAGAGTGGG 1561 GGAACGCGCT GAAGGGGTGT GATGATCCCC TTTTCCTTGA CTTCTTAAAA CAGTGTTTAG 1621 AGTGGGATCC TGCAGTGCGC ATGACCCCAG GCCAGGCTTT GCGGCACCCC TGGCTGAGGA 1681 GGCGGTTGCC AAAGCCTCCC ACCGGGGAGA AAACGTCAGT GAAAAGGATA ACTGAGAGCA 1741 CCGGTGCTAT CACATCTATA TCCAAGTTAC CTCCACCTTC TAGCTCAGCT TCCAAACTGA 1801 GGACTAATTT GGCGCAGATG ACAGATGCCA ATGGGAATAT TCAGCAGAGG ACAGTGTTGC 1861 CAAAACTTGT TAGCTGAGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1921 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1981 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAAGCCGA TGGAAGGTTA 2041 ATACAGTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt