Construct: ORF TRCN0000489008

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020168.1_s317c1
DNA Barcode:
AGCCACGCGCCTATAATTAAACAA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
PKM (5315)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489008
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5315 PKM pyruvate kinase M1/2 NM_002654.6 100% 100%
2 human 5315 PKM pyruvate kinase M1/2 XM_005254445.5 100% 100%
3 human 5315 PKM pyruvate kinase M1/2 NM_182470.3 95.8% 95.8% (many diffs)
4 human 5315 PKM pyruvate kinase M1/2 NM_182471.4 95.8% 95.8% (many diffs)
5 human 5315 PKM pyruvate kinase M1/2 XM_017022313.2 95.8% 95.8% (many diffs)
6 human 5315 PKM pyruvate kinase M1/2 NM_001206799.2 95% 94.9% (many diffs)
7 human 5315 PKM pyruvate kinase M1/2 XM_011521670.2 93.8% 93.8% 1_105del
8 human 5315 PKM pyruvate kinase M1/2 NM_001206798.2 91.5% 80.9% 1_47del;200_201ins92
9 human 5315 PKM pyruvate kinase M1/2 NM_001316318.2 90% 89.9% (many diffs)
10 human 5315 PKM pyruvate kinase M1/2 XM_005254443.1 89.8% 89.8% 1_180del
11 human 5315 PKM pyruvate kinase M1/2 XM_006720570.1 87.7% 87.7% 1_222del
12 human 5315 PKM pyruvate kinase M1/2 NM_001206797.2 86% 86% 171_172ins222
13 human 5315 PKM pyruvate kinase M1/2 NM_001206796.3 84.2% 84.1% (many diffs)
14 human 5315 PKM pyruvate kinase M1/2 XR_001751314.2 54.4% 1_139del;1279_1846del;2301_2924del
15 mouse 18746 Pkm pyruvate kinase, muscle NM_011099.3 90.7% 97.7% (many diffs)
16 mouse 18746 Pkm pyruvate kinase, muscle XM_006510855.1 90.7% 97.7% (many diffs)
17 mouse 18746 Pkm pyruvate kinase, muscle NM_001253883.1 86.9% 93.7% (many diffs)
18 mouse 18746 Pkm pyruvate kinase, muscle XM_011242673.1 86.9% 93.7% (many diffs)
19 mouse 18746 Pkm pyruvate kinase, muscle XM_006510856.2 82.9% 84.6% (many diffs)
20 mouse 18746 Pkm pyruvate kinase, muscle XM_011242674.1 81% 87.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1665
ORF length:
1593
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgtcgaag ccccatagtg aagccgggac tgccttcatt cagacccagc
121agctgcacgc agccatggct gacacattcc tggagcacat gtgccgcctg gacattgatt
181caccacccat cacagcccgg aacactggca tcatctgtac cattggccca gcttcccgat
241cagtggagac gttgaaggag atgattaagt ctggaatgaa tgtggctcgt ctgaacttct
301ctcatggaac tcatgagtac catgcggaga ccatcaagaa tgtgcgcaca gccacggaaa
361gctttgcttc tgaccccatc ctctaccggc ccgttgctgt ggctctagac actaaaggac
421ctgagatccg aactgggctc atcaagggca gcggcactgc agaggtggag ctgaagaagg
481gagccactct caaaatcacg ctggataacg cctacatgga aaagtgtgac gagaacatcc
541tgtggctgga ctacaagaac atctgcaagg tggtggaagt gggcagcaag atctacgtgg
601atgatgggct tatttctctc caggtgaagc agaaaggtgc cgacttcctg gtgacggagg
661tggaaaatgg tggctccttg ggcagcaaga agggtgtgaa ccttcctggg gctgctgtgg
721acttgcctgc tgtgtcggag aaggacatcc aggatctgaa gtttggggtc gagcaggatg
781ttgatatggt gtttgcgtca ttcatccgca aggcatctga tgtccatgaa gttaggaagg
841tcctgggaga gaagggaaag aacatcaaga ttatcagcaa aatcgagaat catgaggggg
901ttcggaggtt tgatgaaatc ctggaggcca gtgatgggat catggtggct cgtggtgatc
961taggcattga gattcctgca gagaaggtct tccttgctca gaagatgatg attggacggt
1021gcaaccgagc tgggaagcct gtcatctgtg ctactcagat gctggagagc atgatcaaga
1081agccccgccc cactcgggct gaaggcagtg atgtggccaa tgcagtcctg gatggagccg
1141actgcatcat gctgtctgga gaaacagcca aaggggacta tcctctggag gctgtgcgca
1201tgcagcacct gattgcccgt gaggcagagg ctgccatcta ccacttgcaa ttatttgagg
1261aactccgccg cctggcgccc attaccagcg accccacaga agccaccgcc gtgggtgccg
1321tggaggccTC CTTCAAGTGC TGCAGTGGGG CCATAATCGT CCTCACCAAG TCTGGCAGGT
1381CTGCTCACCA GGTGGCCAGA TACCGCCCAC GTGCCCCCAT CATTGCTGTG ACCCGGAATC
1441CCCAGACAGC TCGTCAGGCC CACCTGTACC GTGGCATCTT CCCTGTGCTG TGCAAGGACC
1501CAGTCCAGGA GGCCTGGGCT GAGGACGTGG ACCTCCGGGT GAACTTTGCC ATGAATGTTG
1561GCAAGGCCCG AGGCTTCTTC AAGAAGGGAG ATGTGGTCAT TGTGCTGACC GGATGGCGCC
1621CTGGCTCCGG CTTCACCAAC ACCATGCGTG TTGTTCCTGT GCCGTGAGAC CCAGCTTTCT
1681TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
1741CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
1801GTGGAAAGGA CGAAGCCACG CGCCTATAAT TAAACAAACG CGTTAAGTCg acaatcaacc
1861tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)

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