Construct: ORF TRCN0000489009
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020135.1_s317c1
- DNA Barcode:
- TTCCTCCTCCGGAGGTGGAATTCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- DAPK3 (1613)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489009
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1613 | DAPK3 | death associated protein ki... | NM_001348.3 | 100% | 100% | |
| 2 | human | 1613 | DAPK3 | death associated protein ki... | XM_005259508.2 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1434
- ORF length:
- 1362
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtccacg ttcaggcagg aggacgtgga ggaccattat gagatggggg 121 aggagctggg cagcggccag tttgcgatcg tgcggaagtg ccggcagaag ggcacgggca 181 aggagtacgc agccaagttc atcaagaagc gccgcctgtc atccagccgg cgtggggtga 241 gccgggagga gatcgagcgg gaggtgaaca tcctgcggga gatccggcac cccaacatca 301 tcaccctgca cgacatcttc gagaacaaga cggacgtggt cctcatcctg gagctggtct 361 ctggcgggga gctctttgac ttcctggcgg agaaggagtc gctgacggag gacgaggcca 421 cccagttcct caagcagatc ctggacggcg ttcactacct gcactctaag cgcatcgcac 481 actttgacct gaagccggaa aacatcatgc tgctggacaa gaacgtgccc aacccacgaa 541 tcaagctcat cgacttcggc atcgcgcaca agatcgaggc ggggaacgag ttcaagaaca 601 tcttcggcac cccggagttt gtggccccag agattgtgaa ctatgagccg ctgggcctgg 661 aggcggacat gtggagcatc ggtgtcatca cctatatcct cctgagcggt gcatccccgt 721 tcctgggcga gaccaagcag gagacgctca ccaacatctc agccgtgaac tacgacttcg 781 acgaggagta cttcagcaac accagcgagc tggccaagga cttcattcgc cggctgctcg 841 tcaaagatcc caagcggaga atgaccattg cccagagcct ggaacattcc tggattaagg 901 cgatccggcg gcggaacgtg cgtggtgagg acagcggccg caagcccgag cggcggcgcc 961 tgaagaccac gcgtctgaag gagtacacca tcaagtcgca ctccagcttg ccgcccaaca 1021 acagctacgc cgacttcgag cgcttctcca aggtgctgga ggaggcggcg gccgccgagg 1081 agggcctgcg cgagctgcag cgcagccGGC GGCTCTGCCA CGAGGACGTG GAGGCGCTGG 1141 CCGCCATCTA CGAGGAGAAG GAGGCCTGGT ACCGCGAGGA GAGCGACAGC CTGGGCCAGG 1201 ACCTGCGGAG GCTACGGCAG GAGCTGCTCA AGACCGAGGC GCTCAAGCGG CAGGCGCAGG 1261 AGGAGGCCAA GGGCGCGCTG CTGGGGACCA GCGGCCTCAA GCGCCGCTTC AGCCGCCTGG 1321 AGAACCGCTA CGAGGCGCTG GCCAAGCAAG TAGCCTCCGA GATGCGCTTC GTGCAGGACC 1381 TCGTGCGCGC CCTGGAGCAG GAGAAGCTGC AGGGCGTGGA GTGCGGGCTG CGCTGAGACC 1441 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1501 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1561 ATATATCTTG TGGAAAGGAC GATTCCTCCT CCGGAGGTGG AATTCCACGC GTTAAGTCga 1621 caatcaacct ctggattaca aaatttgtga aagatt