Transcript: Human NM_001348.3

Homo sapiens death associated protein kinase 3 (DAPK3), mRNA.

Source:
NCBI, updated 2019-07-06
Taxon:
Homo sapiens (human)
Gene:
DAPK3 (1613)
Length:
2286
CDS:
274..1638

Additional Resources:

NCBI RefSeq record:
NM_001348.3
NBCI Gene record:
DAPK3 (1613)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147833 CGTGAACTACGACTTCGACG pXPR_003 AGG 709 52% 7 0.0255 DAPK3 DAPK3 77559
2 BRDN0001162354 GCGGCCAGTTTGCGATCGTG pXPR_003 CGG 78 6% 3 -0.0849 DAPK3 DAPK3 77556
3 BRDN0001147205 TCGCGCACAAGATCGAGGCG pXPR_003 GGG 507 37% 4 -0.1438 DAPK3 DAPK3 77558
4 BRDN0001162290 CTCCTTCCTAGGCGATCCGG pXPR_003 CGG 834 61% 9 -0.1512 DAPK3 DAPK3 77557
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001348.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000320487 CGGGTAGTTGCACGGACAATT pLKO_005 1994 3UTR 100% 13.200 18.480 N DAPK3 n/a
2 TRCN0000000520 CATCGCACACTTTGACCTGAA pLKO.1 675 CDS 100% 4.050 5.670 N DAPK3 n/a
3 TRCN0000381615 GACTTCGACGAGGAGTACTTC pLKO_005 976 CDS 100% 4.950 3.960 N DAPK3 n/a
4 TRCN0000000519 CAACCCACGAATCAAGCTCAT pLKO.1 732 CDS 100% 4.050 3.240 N DAPK3 n/a
5 TRCN0000009959 CGTCTGAAGGAGTACACCATC pLKO.1 1174 CDS 100% 4.050 3.240 N DAPK3 n/a
6 TRCN0000320485 CGTCTGAAGGAGTACACCATC pLKO_005 1174 CDS 100% 4.050 3.240 N DAPK3 n/a
7 TRCN0000320486 ATCGGTGTCATCACCTATATC pLKO_005 880 CDS 100% 13.200 9.240 N DAPK3 n/a
8 TRCN0000009958 CCCAAGCGGAGAATGACCATT pLKO.1 1051 CDS 100% 4.950 3.465 N DAPK3 n/a
9 TRCN0000009945 CGTTCACTACCTGCACTCTAA pLKO.1 651 CDS 100% 4.950 3.465 N DAPK3 n/a
10 TRCN0000055426 CGTTCACTACCTGCACTCTAA pLKO.1 651 CDS 100% 4.950 3.465 N DAPK3 n/a
11 TRCN0000320484 CGTTCACTACCTGCACTCTAA pLKO_005 651 CDS 100% 4.950 3.465 N DAPK3 n/a
12 TRCN0000199874 GCACGACATCTTCGAGAACAA pLKO.1 510 CDS 100% 4.950 3.465 N DAPK3 n/a
13 TRCN0000380221 TGCACGACATCTTCGAGAACA pLKO_005 509 CDS 100% 4.950 3.465 N DAPK3 n/a
14 TRCN0000009944 CAAGCAAGTAGCCTCCGAGAT pLKO.1 1545 CDS 100% 4.050 2.835 N DAPK3 n/a
15 TRCN0000000522 GAAGGAGTACACCATCAAGTC pLKO.1 1179 CDS 100% 4.050 2.835 N DAPK3 n/a
16 TRCN0000000521 TGTCATCACCTATATCCTCCT pLKO.1 885 CDS 100% 2.160 1.512 N DAPK3 n/a
17 TRCN0000199436 GTGAACATCCTGCGGGAGATC pLKO.1 466 CDS 100% 1.350 0.945 N DAPK3 n/a
18 TRCN0000009954 AGATTGTGAACTATGAGCCGC pLKO.1 833 CDS 100% 0.540 0.378 N DAPK3 n/a
19 TRCN0000320414 TTGACTTCCTGGCGGAGAAAG pLKO_005 578 CDS 100% 10.800 7.560 N DAPK3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001348.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489009 TTCCTCCTCCGGAGGTGGAATTCC pLX_317 24.4% 100% 100% V5 (not translated due to prior stop codon) n/a
2 ccsbBroadEn_14610 pDONR223 100% 98.6% 41.4% None (many diffs) n/a
3 ccsbBroad304_14610 pLX_304 0% 98.6% 41.4% V5 (not translated due to prior stop codon) (many diffs) n/a
4 TRCN0000468144 TACAGTGCAGAGCATGATATCCCG pLX_317 28.6% 98.6% 41.4% V5 (not translated due to prior stop codon) (many diffs) n/a
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