Construct: ORF TRCN0000489026
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021487.1_s317c1
- DNA Barcode:
- TGCGCGCTGGAATAGGGCGCAATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- XPO5 (57510)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489026
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57510 | XPO5 | exportin 5 | NM_020750.3 | 99.9% | 100% | 3303C>T |
| 2 | human | 57510 | XPO5 | exportin 5 | NR_144392.1 | 65.6% | (many diffs) | |
| 3 | mouse | 72322 | Xpo5 | exportin 5 | NM_028198.3 | 86.5% | 87.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3684
- ORF length:
- 3612
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcgatg gatcaagtaa acgcgctgtg cgagcagctg gtgaaagcgg 121 tgacggtcat gatggacccc aactccaccc agcgctaccg gctggaagcc ctcaagtttt 181 gtgaggagtt taaagaaaag tgtcctatct gtgtcccctg tggcttgagg ttggctgaga 241 aaacacaagt tgccatcgtc agacattttg gccttcagat cctggaacac gttgtcaagt 301 ttcggtggaa cggcatgtct cgattggaga aggtgtatct gaagaacagt gtcatggagc 361 tgattgcaaa tggaacattg aacattttgg aagaggagaa ccatattaaa gatgctctgt 421 ctcgaattgt agtggaaatg atcaagcgag agtggccaca gcattggcct gacatgctaa 481 tagaattgga cactctttcc aaacaagggg aaacacagac agaattggtg atgtttatcc 541 ttttgcgact ggcagaggat gtagtgactt ttcagacact tccccctcaa agaagaaggg 601 acatccagca aacattaacc cagaacatgg aaaggatctt cagttttctg cttaacacac 661 ttcaagaaaa tgtaaacaag tatcagcaag tgaagacaga tacttctcag gagtcaaagg 721 cgcaagcaaa ctgtcgagta ggagttgcag cactgaatac tctagcaggc tatattgact 781 gggtgtctat gagtcacatc actgctgaaa actgtaaact cctggagata ctgtgtttgc 841 tgttgaatga acaggaactt cagttgggag ccgctgagtg tcttctcatt gcagtcagca 901 gaaaaggcaa gttggaagac cggaagccct tgatggtctt atttggagat gttgccatgc 961 attatatact ctccgccgca cagactgctg atggaggagg tttggtagaa aaacactacg 1021 tctttctgaa gaggctctgt caggtgttgt gtgcgctggg caatcagctg tgtgcattgc 1081 tgggtgcaga ttctgatgta gaaacaccat caaactttgg aaaatacctg gaatcttttc 1141 ttgctttcac aacccatcca agtcagtttc tacgctcttc aactcagatg acttggggag 1201 ccctcttcag gcatgaaatc ctgtcccgtg atcctttgct attagcaata ataccaaaat 1261 atcttcgtgc ttccatgact aacttggtca agatgggctt tccttctaaa acagacagcc 1321 ctagctgtga atattctcgg tttgattttg atagcgatga ggacttcaat gctttcttca 1381 actcctcccg agcacaacaa ggagaggtga tgaggttggc atgtcgtttg gatcccaaaa 1441 ctagcttcca gatggctggg gagtggctaa agtatcaact atcaactttt cttgatgctg 1501 gttctgtgaa ttcttgttct gcagttggaa ctggagaagg aagcctctgt tccgtcttct 1561 caccttcatt cgtgcagtgg gaagccatga ctcttttttt ggaaagtgtt atcacccaga 1621 tgtttcgaac actaaataga gaagaaattc ctgttaatga tggaatagag ctattgcaga 1681 tggttctgaa ctttgatacc aaggatcccc tcatcctgtc ctgcgtcctt actaatgtct 1741 ctgcactctt tccatttgtc acctacagac cagagttcct gccccaggtc ttctctaagc 1801 tattttcatc tgtcactttt gaaactgttg aagaaagtaa ggcccccaga acccgggcag 1861 tgaggaatgt gaggaggcat gcttgttcct ccatcatcaa gatgtgtcgt gactaccccc 1921 agcttgtgct gcccaatttt gacatgcttt ataaccatgt gaagcaactc ctctccaatg 1981 agctactcct gacacaaatg gagaagtgtg ccctcatgga agccctggtt ctcattagca 2041 accaatttaa gaactacgag cgtcagaagg tgttcctaga ggagctgatg gcaccagtgg 2101 ccagcatctg gctttctcaa gacatgcaca gagtgctgtc agatgttgat gctttcattg 2161 cgtatgtggg tacagatcag aagagctgtg acccaggcct ggaggatccg tgtggcttaa 2221 accgtgcacg aatgagcttt tgtgtataca gcattctggg tgtggtgaaa cgaacttgct 2281 ggcccactga cctagaagag gccaaagctg ggggatttgt ggtgggttat acatccagtg 2341 gaaatccaat cttccgtaac ccctgcacag agcagattct gaaacttctt gacaatttgc 2401 ttgcgcttat aagaacccac aatacattat atgcaccaga aatgctagcc aaaatggcag 2461 agcctttcac caaggctctg gatatgcttg acgcggaaaa atctgctata ttaggattac 2521 ctcaacctct cttggaactc aatgactctc ctgtcttcaa aaccgtcttg gaaagaatgc 2581 agcgtttctt ctctaccctc tatgaaaact gttttcatat cctagggaag gcaggccctt 2641 ccatgcagca agacttctat actgtggagg accttgctac ccagcttctc agctcagcct 2701 ttgtcaactt gaacaatatt cctgactacc gactcagacc catgcttcgt gtctttgtaa 2761 agcctctggt gctcttctgt cccccagagc actatgaagc cctggtatcc cccatcctcg 2821 gacctctttt cacctacctc catatgaggc tttctcagaa atggcaagtt atcaaccaaa 2881 ggagcctgct gtgtggagaa gatgaggctg cagatgaaaa cccagagtct caagagatgc 2941 tggaggagca actggtgagg atgttaaccc gagaagtcat ggacctaatc acggtttgct 3001 gtgtttcaaa gaagggtgct gaccacagta gtgctccccc agcagatgga gacgatgaag 3061 aaatgatggc cacagaggtc accccctcag ctatggcaga gcttacagac ctgggcaaat 3121 gtctgatgaa gcatgaggat gtttgtacag cgctattaat tacagccttc aattccctgg 3181 cctggaaaga tactctgtcc tgccagagga caacctcaca gctctgctgg cctctcctca 3241 aacaagtgct gtcagggaca ctgctcgcag atgcagttac gtggcttttc accagtgtgc 3301 tgaaaggctt acagatgcac gggcagcacg acgggtgcat ggcttcccTG GTCCATCTGG 3361 CCTTCCAGAT ATATGAGGCA CTGCGCCCCA GGTACCTGGA GATAAGAGCT GTAATGGAGC 3421 AAATCCCTGA AATACAGAAG GACTCACTGG ACCAGTTTGA CTGCAAGCTT TTAAACCCCT 3481 CCCTGCAGAA AGTGGCTGAC AAGCGCCGAA AGGACCAATT CAAACGCCTC ATTGCTGGTT 3541 GCATTGGGAA ACCCTTGGGA GAGCAGTTCC GAAAAGAAGT TCACATTAAG AATCTTCCCT 3601 CACTTTTCAA AAAAACAAAG CCAATGCTGG AGACGGAGGT GCTGGACAAT GATGGGGGTG 3661 GCCTGGCCAC CATCTTTGAA CCCTGAGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 3721 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 3781 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATGCGCGCT 3841 GGAATAGGGC GCAATCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 3901 aagatt