Construct: ORF TRCN0000489036
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020876.1_s317c1
- DNA Barcode:
- TCAGCCACCGAGATCGGTCCCGTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HTR1D (3352)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489036
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3352 | HTR1D | 5-hydroxytryptamine recepto... | NM_000864.4 | 99.9% | 100% | 585C>T |
| 2 | mouse | 15552 | Htr1d | 5-hydroxytryptamine (seroto... | NM_001285482.1 | 85% | 90.1% | (many diffs) |
| 3 | mouse | 15552 | Htr1d | 5-hydroxytryptamine (seroto... | NM_001285483.1 | 85% | 90.1% | (many diffs) |
| 4 | mouse | 15552 | Htr1d | 5-hydroxytryptamine (seroto... | NM_001285484.1 | 85% | 90.1% | (many diffs) |
| 5 | mouse | 15552 | Htr1d | 5-hydroxytryptamine (seroto... | NM_008309.5 | 85% | 90.1% | (many diffs) |
| 6 | mouse | 15552 | Htr1d | 5-hydroxytryptamine (seroto... | XM_006538577.3 | 85% | 90.1% | (many diffs) |
| 7 | mouse | 15552 | Htr1d | 5-hydroxytryptamine (seroto... | XM_006538578.2 | 85% | 90.1% | (many diffs) |
| 8 | mouse | 15552 | Htr1d | 5-hydroxytryptamine (seroto... | XM_017319986.2 | 85% | 90.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1203
- ORF length:
- 1131
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtcccca ctgaaccagt cagcagaagg ccttccccag gaggcctcca 121 acagatccct gaatgccaca gaaacctcag aggcttggga tcccaggacc ctccaggcgc 181 tcaagatctc ccttgccgtg gtcctttccg tcatcacact ggccacagtc ctctccaatg 241 cctttgtact caccaccatc ttactcacca ggaagctcca cacccctgcc aactacctga 301 ttggctccct ggccaccacc gacctcttgg tttccatctt ggtaatgccc atcagcatcg 361 cctataccat cacccacacc tggaactttg gccaaatctt gtgtgacatc tggctgtcct 421 ctgacatcac gtgctgcaca gcctccatcc tgcatctctg tgtcattgct ctggacaggt 481 actgggcaat cacagatgcc ctggaataca gtaaacgcag gacggctggc cacgcggcca 541 ccatgatcgc cattgtctgg gccatctcca tctgcatctc catccccccg ctcttctggc 601 ggcaggccaa ggcccaggag gagatgtcgg actgtctggt gaacacctct cagatttcct 661 acaccatcta ctccacctgt ggggccttct acattccctc ggtgttgctc atcatcctat 721 atggccggat ctaccgggct gcccggaacc gcatcctgaa tccaccctca ctctatggga 781 agcgcttcac cacggcccac ctcatcacag gctctgccgg gtcctcgcTC TGCTCGCTCA 841 ACTCCAGCCT CCATGAGGGG CACTCGCACT CGGCTGGCTC CCCTCTCTTT TTCAACCACG 901 TGAAAATCAA GCTTGCTGAC AGTGCCCTGG AACGCAAGAG GATTTCTGCT GCTCGAGAAA 961 GGAAAGCCAC TAAAATCCTG GGCATCATTC TGGGGGCCTT TATCATCTGC TGGCTGCCCT 1021 TCTTCGTGGT GTCTCTGGTC CTCCCCATCT GCCGGGACTC CTGCTGGATC CACCCGGCGC 1081 TCTTTGACTT CTTCACCTGG CTAGGCTATT TAAACTCCCT CATCAATCCA ATAATCTACA 1141 CTGTGTTTAA TGAAGAGTTT CGGCAAGCTT TTCAGAAAAT TGTCCCTTTC CGGAAGGCCT 1201 CCTAGGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1261 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1321 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATCAGCCACC GAGATCGGTC CCGTAACGCG 1381 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt