Construct: ORF TRCN0000489037
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020758.1_s317c1
- DNA Barcode:
- TTGACCCATTGCCTCTATGAAGTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GPR101 (83550)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489037
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83550 | GPR101 | G protein-coupled receptor 101 | NM_054021.1 | 99.9% | 99.8% | 370G>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1596
- ORF length:
- 1524
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgacgtcc acctgcacca acagcacgcg cgagagtaac agcagccaca 121 cgtgcatgcc cctctccaaa atgcccatca gcctggccca cggcatcatc cgctcaaccg 181 tgctggttat cttcctcgcc gcctctttcg tcggcaacat agtgctggcg ctagtgttgc 241 agcgcaagcc gcagctgctg caggtgacca accgttttat ctttaacctc ctcgtcaccg 301 acctgctgca gatttcgctc gtggccccct gggtggtggc cacctctgtg cctctcttct 361 ggcccctcaa cagccacttc tgcacggccc tggttagcct cacccacctg ttcgccttcg 421 ccagcgtcaa caccattgtc ttggtgtcag tggatcgcta cttgtccatc atccaccctc 481 tctcctaccc gtccaagatg acccagcgcc gcggttacct gctcctctat ggcacctgga 541 ttgtggccat cctgcagagc actcctccac tctacggctg gggccaggct gcctttgatg 601 agcgcaatgc tctctgctcc atgatctggg gggccagccc cagctacact attctcagcg 661 tggtgtcctt catcgtcatt ccactgattg tcatgattgc ctgctactcc gtggtgttct 721 gtgcagcccg gaggcagcat gctctgctgt acaatgtcaa gagacacagc ttggaagtgc 781 gagtcaagga ctgtgtggag aatgaggatg aagagggagc agagaagaag gaggagttcc 841 aggatgagag tgagtttcgc cgccagcatg aaggtgaggt caaggccaag gagggcagaa 901 tggaagccaa ggacggcagc ctgaaggcca aggaaggaag cacggggacc agtgagagta 961 gtgtagaggc caggggcagc gaggaggtca gagagagcag cacggtggcc agcgacggca 1021 gcatggaggg taaggaaggc agcaccaaag ttgaggagaa cagcatgaag gcagacaagg 1081 gtcgcacaga ggtcaaccag tgcagcattg acttgggtga agatgacatg gagtttggtg 1141 aagacgacat caatttcagt gaggatgacg tcgaggcagt gaacatcccg gagagcctcc 1201 cacccagtcg tcgtaacagc aacagcaacc ctccTCTGCC CAGGTGCTAC CAGTGCAAAG 1261 CTGCTAAAGT GATCTTCATC ATCATTTTCT CCTATGTGCT ATCCCTGGGG CCCTACTGCT 1321 TTTTAGCAGT CCTGGCCGTG TGGGTGGATG TCGAAACCCA GGTACCCCAG TGGGTGATCA 1381 CCATAATCAT CTGGCTTTTC TTCCTGCAGT GCTGCATCCA CCCCTATGTC TATGGCTACA 1441 TGCACAAGAC CATTAAGAAG GAAATCCAGG ACATGCTGAA GAAGTTCTTC TGCAAGGAAA 1501 AGCCCCCGAA AGAAGATAGC CACCCAGACC TGCCCGGAAC AGAGGGTGGG ACTGAAGGCA 1561 AGATTGTCCC TTCCTACGAT TCTGCTACTT TTCCTTAGGA CCCAGCTTTC TTGTACAAAG 1621 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1681 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1741 ACGATTGACC CATTGCCTCT ATGAAGTTAC GCGTTAAGTC gacaatcaac ctctggatta 1801 caaaatttgt gaaagatt