Construct: ORF TRCN0000489047
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019620.1_s317c1
- DNA Barcode:
- CCACGAGTGGGCTGCTCTATGGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OR6B2 (389090)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489047
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 389090 | OR6B2 | olfactory receptor family 6... | NM_001005853.1 | 99.8% | 99.6% | 936_937insG |
| 2 | human | 150681 | OR6B3 | olfactory receptor family 6... | NM_173351.1 | 90.9% | 88.5% | (many diffs) |
| 3 | human | 150681 | OR6B3 | olfactory receptor family 6... | XM_011510671.2 | 68.7% | 66.8% | (many diffs) |
| 4 | mouse | 259040 | Olfr1416 | olfactory receptor 1416 | NM_147038.1 | 86.2% | 85.6% | (many diffs) |
| 5 | mouse | 259041 | Olfr1414 | olfactory receptor 1414 | NM_147039.2 | 85.9% | 86.5% | (many diffs) |
| 6 | mouse | 258228 | Olfr1415 | olfactory receptor 1415 | NM_001011525.1 | 85.3% | 85.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1014
- ORF length:
- 939
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgagt ggggagaatg tcaccaaggt cagcaccttc atcctggtgg 121 gcctccccac ggccccaggg ctgcagtacc tgctcttcct cctcttcctg ctcacctacc 181 tctttgtcct ggtggagaac ctggccatca tcctcatcgt ctggagcagc acctccctcc 241 acaggcccat gtactacttt ctgagctcca tgtctttcct ggagatctgg tacgtgtctg 301 acatcacccc caagatgctg gagggcttcc tcctccagca gaaacgcatc tctttcgtcg 361 ggtgcatgac gcagctctac ttcttcagct ccctggtgtg caccgagtgt gtgcttctgg 421 cctccatggc ctacgaccgc tacgtggcca tctgccaccc gctgcgctac cacgtccttg 481 tgaccccggg gctgtgcctc cagctggtgg gcttctcctt tgtgagtggc ttcaccatct 541 ccatgatcaa ggtctgtttt atctccagcg tcacgttctg tggctccaac gtcttgaacc 601 acttcttctg tgacatttcc cccatcctca agctggcctg cacggacttc tccactgcag 661 agctggtgga tttcatcctg gCCTTCATCA TCCTGGTGTT TCCGCTCCTG GCCACCATAC 721 TGTCATATTG GCACATCACC CTGGCTGTCC TGCGCATCCC CTCGGCCACC GGCTGCTGGA 781 GAGCCTTCTC TACCTGCGCC TCTCACCTCA CCGTGGTCAC CGTCTTCTAT ACAGCCTTGC 841 TTTTCATGTA TGTCCGGCCC CAAGCCATTG ATTCCCAGAG CTCCAACAAG CTCATCTCTG 901 CCGTGTACAC TGTTGTCACG CCAATAATTA ACCCTTTGAT TTACTGCCTG AGGAACAAGG 961 AATTTAAGGA CGCCTTGAAA AAGGCCTTGG GCTTGGGTCA AACTTCACAC GACCCAGCTT 1021 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1081 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1141 CTTGTGGAAA GGACGACCAC GAGTGGGCTG CTCTATGGTC ACGCGTTAAG TCgacaatca 1201 acctctggat tacaaaattt gtgaaagatt