Construct: ORF TRCN0000489049
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019625.1_s317c1
- DNA Barcode:
- CAGCTCATACGTTCTCGATGAGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GHSR (2693)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489049
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2693 | GHSR | growth hormone secretagogue... | NM_004122.2 | 99.8% | 99.6% | 867_868insG |
| 2 | human | 2693 | GHSR | growth hormone secretagogue... | NM_198407.2 | 77.2% | 72.2% | (many diffs) |
| 3 | mouse | 208188 | Ghsr | growth hormone secretagogue... | NM_177330.4 | 70.1% | 68.5% | (many diffs) |
| 4 | mouse | 208188 | Ghsr | growth hormone secretagogue... | XM_017319513.1 | 59.8% | 58.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 945
- ORF length:
- 870
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgtgg aacgcgacgc ccagcgaaga gccggggttc aacctcacac 121 tggccgacct ggactgggat gcttcccccg gcaacgactc gctgggcgac gagctgctgc 181 agctcttccc cgcgccgctg ctggcgggcg tcacagccac ctgcgtggca ctcttcgtgg 241 tgggcatcgc tggcaacctg ctcaccatgc tggtggtgtc gcgcttccgc gagctgcgca 301 ccaccaccaa cctctacctg tccagcatgg ccttctccga tctgctcatc ttcctctgca 361 tgcccctgga cctcgttcgc ctctggcagt accggccctg gaacttcggc gacctcctct 421 gcaaactctt ccaattcgtc agtgagagct gcacctacgc cacggtgctc accatcacag 481 cgctgagcgt cgagcgctac ttcgccatct gcttcccact ccgggccaag gtggtggtca 541 ccaaggggcg ggtgaagctg gtcatcttcg tcatctgggc cgtggccTTC TGCAGCGCCG 601 GGCCCATCTT CGTGCTAGTC GGGGTGGAGC ACGAGAACGG CACCGACCCT TGGGACACCA 661 ACGAGTGCCG CCCCACCGAG TTTGCGGTGC GCTCTGGACT GCTCACGGTC ATGGTGTGGG 721 TGTCCAGCAT CTTCTTCTTC CTTCCTGTCT TCTGTCTCAC GGTCCTCTAC AGTCTCATCG 781 GCAGGAAGCT GTGGCGGAGG AGGCGCGGCG ATGCTGTCGT GGGTGCCTCG CTCAGGGACC 841 AGAACCACAA GCAAACCGTG AAAATGCTGG GTGGGTCTCA GCGCGCGCTC AGGCTTTCTC 901 TCGCGGGTCC TATCCTCTCC CTGTGCCTTC TCCCTTCTCT CGACCCAGCT TTCTTGTACA 961 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1021 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1081 AGGACGACAG CTCATACGTT CTCGATGAGC TACGCGTTAA GTCgacaatc aacctctgga 1141 ttacaaaatt tgtgaaagat t