Construct: ORF TRCN0000489056
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021626.1_s317c1
- DNA Barcode:
- CCATAGAGTTGCCGGGGTCTGTTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PCSK9 (255738)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489056
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 255738 | PCSK9 | proprotein convertase subti... | NM_174936.4 | 75.1% | 75% | (many diffs) |
| 2 | human | 255738 | PCSK9 | proprotein convertase subti... | NR_110451.1 | 49.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1638
- ORF length:
- 1569
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gtcgccttgg aaagacggag gcagcctggt ggaggtgtat ctcctagaca 121 ccagcataca gagtgaccac cgggaaatcg agggcagggt catggtcacc gacttcgaga 181 atgtgcccga ggaggacggg acccgcttcc acagacaggc cagcaagtgt gacagtcatg 241 gcacccacct ggcaggggtg gtcagcggcc gggatgccgg cgtggccaag ggtgccagca 301 tgcgcagcct gcgcgtgctc aactgccaag ggaagggcac ggttagcggc accctcatag 361 gcctggagtt tattcggaaa agccagctgg tccagcctgt ggggccactg gtggtgctgc 421 tgcccctggc gggtgggtac agccgcgtcc tcaacgccgc ctgccagcgc ctggcgaggg 481 ctggggtcgt gctggtcacc gctgccggca acttccggga cgatgcctgc ctctactccc 541 cagcctcagc tcccgaggtc atcacagttg gggccaccaa tgcccaggac cagccggtga 601 ccctggggac tttggggacc aactttggcc gctgtgtgga cctctttgcc ccaggggagg 661 acatcattgg tgcctccagc gactgcagca cctgctttgt gtcacagagt gggacatcac 721 aggctgctgc ccacgtggct ggcattgcag ccatgatgct gtctgccgag ccggagctca 781 ccctggccga gttgaggcag agactgatcc acttctctgc caaagatgtc atcaatgagg 841 cctggttccc tgaggaccag cgggtactga cccccaacct ggtggccgcc ctgcccccca 901 gcacccatgg ggcaggttgg cagctgtttt gcaggactgt gtggtcagca cactcggggc 961 ctacacggat ggccacagcc atcgcccgct gcgccccaga tgaggagctg ctgagctgct 1021 ccagtttctc caggagtggg aagcggcggg gcgagcgcat ggaggcccaa gggggcaagc 1081 tggtctgccg ggcccacaac gcttttgggg gtgagggtgt ctacgccatt gccaggtgct 1141 gcctgctacc ccaggccaac tgcagcgtcc acacagctcc accagctgag gccagcatgg 1201 ggacccgtgt ccactgccac caacagggcc acgtcctcac aggctgcagc tcccactggg 1261 aggtggagga ccttggcacc cacaagccgc cTGTGCTGAG GCCACGAGGT CAGCCCAACC 1321 AGTGCGTGGG CCACAGGGAG GCCAGCATCC ACGCTTCCTG CTGCCATGCC CCAGGTCTGG 1381 AATGCAAAGT CAAGGAGCAT GGAATCCCGG CCCCTCAGGA GCAGGTGACC GTGGCCTGCG 1441 AGGAGGGCTG GACCCTGACT GGCTGCAGTG CCCTCCCTGG GACCTCCCAC GTCCTGGGGG 1501 CCTACGCCGT AGACAACACG TGTGTAGTCA GGAGCCGGGA CGTCAGCACT ACAGGCAGCA 1561 CCAGCGAAGG GGCCGTGACA GCCGTTGCCA TCTGCTGCCG GAGCCGGCAC CTGGCGCAGG 1621 CCTCCCAGGA GCTCCAGTGA GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1681 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1741 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACCAT AGAGTTGCCG 1801 GGGTCTGTTA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt