Construct: ORF TRCN0000489073
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020594.1_s317c1
- DNA Barcode:
- TCCAGCACATTACAGTTGTGTTCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- BUB1 (699)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489073
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 699 | BUB1 | BUB1 mitotic checkpoint ser... | NM_004336.5 | 100% | 100% | |
| 2 | human | 699 | BUB1 | BUB1 mitotic checkpoint ser... | NM_001278616.1 | 98.1% | 98.1% | 26_27ins60 |
| 3 | human | 699 | BUB1 | BUB1 mitotic checkpoint ser... | NM_001278617.1 | 94.7% | 94.7% | 2625_2626ins171 |
| 4 | human | 699 | BUB1 | BUB1 mitotic checkpoint ser... | XR_923001.3 | 93% | 1_68del;2530_2570del;3365_3499del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3327
- ORF length:
- 3255
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggacacc ccggaaaatg tccttcagat gcttgaagcc cacatgcaga 121 gctacaaggg caatgaccct cttggtgaat gggaaagata catacagtgg gtagaagaga 181 attttcctga gaataaagaa tacttgataa ctttactaga acatttaatg aaggaatttt 241 tagataagaa gaaataccac aatgacccaa gattcatcag ttattgttta aaatttgctg 301 agtacaacag tgacctccat caattttttg agtttctgta caaccatggg attggaaccc 361 tgtcatcccc tctgtacatt gcctgggcgg ggcatctgga agcccaagga gagctgcagc 421 atgccagtgc tgtccttcag agaggaattc aaaaccaggc tgaacccaga gagttcctgc 481 aacaacaata caggttattt cagacacgcc tcactgaaac ccatttgcca gctcaagcta 541 gaacctcaga acctctgcat aatgttcagg ttttaaatca aatgataaca tcaaaatcaa 601 atccaggaaa taacatggcc tgcatttcta agaatcaggg ttcagagctt tctggagtga 661 tatcttcagc ttgtgataaa gagtcaaata tggaacgaag agtgatcacg atttctaaat 721 cagaatattc tgtgcactca tctttggcat ccaaagttga tgttgagcag gttgttatgt 781 attgcaagga gaagcttatt cgtggggaat cagaattttc ctttgaagaa ttgagagccc 841 agaaatacaa tcaacggaga aagcatgagc aatgggtaaa tgaagacaga cattatatga 901 aaaggaaaga agcaaatgct tttgaagaac agctattaaa acagaaaatg gatgaacttc 961 ataagaagtt gcatcaggtg gtggagacat cccatgagga tctgcccgct tcccaggaaa 1021 ggtccgaggt taatccagca cgtatggggc caagtgtagg ctcccagcag gaactgagag 1081 cgccatgtct tccagtaacc tatcagcaga caccagtgaa catggaaaag aacccaagag 1141 aggcacctcc tgttgttcct cctttggcaa atgctatttc tgcagctttg gtgtccccag 1201 ccaccagcca gagcattgct cctcctgttc ctttgaaagc ccagacagta acagactcca 1261 tgtttgcagt ggccagcaaa gatgctggat gtgtgaataa gagtactcat gaattcaagc 1321 cacagagtgg agcagagatc aaagaagggt gtgaaacaca taaggttgcc aacacaagtt 1381 cttttcacac aactccaaac acatcactgg gaatggttca ggcaacgcca tccaaagtgc 1441 agccatcacc caccgtgcac acaaaagaag cattaggttt catcatgaat atgtttcagg 1501 ctcctacact tcctgatatt tctgatgaca aagatgaatg gcaatctcta gatcaaaatg 1561 aagatgcatt tgaagcccag tttcaaaaaa atgtaaggtc atctggggct tggggagtca 1621 ataagatcat ctcttctttg tcatctgctt ttcatgtgtt tgaagatgga aacaaagaaa 1681 attatggatt accacagcct aaaaataaac ccacaggagc caggaccttt ggagaacgct 1741 ctgtcagcag acttccttca aaaccaaagg aggaagtgcc tcatgctgaa gagtttttgg 1801 atgactcaac tgtatggggt attcgctgca acaaaaccct ggcacccagt cctaagagcc 1861 caggagactt cacatctgct gcacaacttg cgtctacacc attccacaag cttccagtgg 1921 agtcagtgca cattttagaa gataaagaaa atgtggtagc aaaacagtgt acccaggcga 1981 ctttggattc ttgtgaggaa aacatggtgg tgccttcaag ggatggaaaa ttcagtccaa 2041 ttcaagagaa aagcccaaaa caggccttgt cgtctcacat gtattcagca tccttacttc 2101 gtctgagcca gcctgctgca ggtggggtac ttacctgtga ggcagagttg ggcgttgagg 2161 cttgcagact cacagacact gacgctgcca ttgcagaaga tccaccagat gctattgctg 2221 ggctccaagc agaatggatg cagatgagtt cacttgggac tgttgatgct ccaaacttca 2281 ttgttgggaa cccatgggat gataagctga ttttcaaact tttatctggg ctttctaaac 2341 cagtgagttc ctatccaaat acttttgaat ggcaatgtaa acttccagcc atcaagccca 2401 agactgaatt tcaattgggt tctaagctgg tctatgtcca tcaccttctt ggagaaggag 2461 cctttgccca ggtgtacgaa gctacccagg gagatctgaa tgatgctaaa aataaacaga 2521 aatttgtttt aaaggtccaa aagcctgcca acccctggga attctacatt gggacccagt 2581 tgatggaaag actaaagcca tctatgcagc acatgtttat gaagttctat tctgcccact 2641 tattccagaa tggcagtgta ttagtaggag agctctacag ctatggaaca ttattaaatg 2701 ccattaacct ctataaaaat acccctgaaa aagtgatgcc tcaaggtctt gtcatctctt 2761 ttgctatgag aatgctttac atgattgagc aagtgcatga ctgtgaaatc attcatggag 2821 acattaaacc agacaatttc atacttggaa acggattttt ggaacaggat gatgaagatg 2881 atttatctgc tggcttggca ctgattgacc tgggtcagag tatagatatg aaactttttc 2941 caaaaggaac tatattcaca gcaaagtgtg aaacatctgg ttttcagtgt gttgagatgc 3001 tcagcaacaa accatGGAAC TACCAGATCG ATTACTTTGG GGTTGCTGCA ACAGTATATT 3061 GCATGCTCTT TGGCACTTAC ATGAAAGTGA AAAATGAAGG AGGAGAGTGT AAGCCTGAAG 3121 GTCTTTTTAG AAGGCTTCCT CATTTGGATA TGTGGAATGA ATTTTTTCAT GTTATGTTGA 3181 ATATTCCAGA TTGTCATCAT CTTCCATCTT TGGATTTGTT AAGGCAAAAG CTGAAGAAAG 3241 TATTTCAACA ACACTATACT AACAAGATTA GGGCCCTACG TAATAGGCTA ATTGTACTGC 3301 TCTTAGAATG TAAGCGTTCA CGAAAATAAG ACCCAGCTTT CTTGTACAAA GTGGTTGATA 3361 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 3421 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATCCAG 3481 CACATTACAG TTGTGTTCTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 3541 tgaaagatt