Construct: ORF TRCN0000489074
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020485.1_s317c1
- DNA Barcode:
- ATCCTCGGACATCTAGAGCCCTCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RPS6KA4 (8986)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489074
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8986 | RPS6KA4 | ribosomal protein S6 kinase A4 | NM_003942.3 | 99.9% | 99.8% | 1285C>T |
| 2 | human | 8986 | RPS6KA4 | ribosomal protein S6 kinase A4 | NM_001006944.2 | 99.1% | 99% | 1197_1198insCAGGACTCGCCCTTCTTC;1267C>T |
| 3 | human | 8986 | RPS6KA4 | ribosomal protein S6 kinase A4 | NM_001300802.2 | 99% | 98.9% | 1196_1197ins21;1264C>T |
| 4 | human | 8986 | RPS6KA4 | ribosomal protein S6 kinase A4 | XM_005274380.1 | 92.8% | 92.7% | 903_904ins165;1120C>T |
| 5 | human | 8986 | RPS6KA4 | ribosomal protein S6 kinase A4 | NM_001318361.2 | 91.7% | 91.7% | 0_1ins189;1096C>T |
| 6 | human | 8986 | RPS6KA4 | ribosomal protein S6 kinase A4 | XM_017018527.2 | 51.5% | 51.4% | 0_1ins1122;163C>T |
| 7 | mouse | 56613 | Rps6ka4 | ribosomal protein S6 kinase... | NM_019924.2 | 88% | 95.8% | (many diffs) |
| 8 | mouse | 56613 | Rps6ka4 | ribosomal protein S6 kinase... | XM_017318261.1 | 80.6% | 88.3% | (many diffs) |
| 9 | mouse | 56613 | Rps6ka4 | ribosomal protein S6 kinase... | XM_017318262.1 | 76.4% | 82.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2388
- ORF length:
- 2316
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggggac gaggacgacg atgagagctg cgccgtggag ctgcggatca 121 cagaagccaa cctgaccggg cacgaggaga aggtgagcgt ggagaacttc gagctgctca 181 aggtgctggg cacgggagcc tacggcaagg tgttcctggt gcggaaggcg ggcgggcacg 241 acgcggggaa gctgtacgcc atgaaggtgc tgcgcaaggc ggcgctggtg cagcgcgcca 301 agacgcaaga gcacacgcgc accgagcgct cggtgctgga gctggtgcgc caggcgccct 361 tcctggtcac gctgcactac gctttccaga cggatgccaa gctgcacctc atcctggact 421 atgtgagcgg cggggagatg ttcacccacc tctaccagcg ccagtacttc aaggaggctg 481 aggtgcgcgt gtatgggggt gagatcgtgc tggccctgga acacctgcac aagctcggca 541 tcatttaccg agacctgaaa ctggagaatg tgctgctgga ctccgagggc cacattgtcc 601 tcacggactt cgggctgagc aaggagttcc tgacggagga gaaagagcgg accttctcct 661 tctgtggcac catcgagtac atggcccccg aaatcatccg tagcaagacg gggcatggca 721 aggctgtgga ctggtggagc ctgggcatct tgctcttcga gctgctgacg ggggcctcgc 781 ccttcaccct ggagggcgag aggaacacgc aggctgaggt gtctcgacgg atcctgaagt 841 gctcccctcc cttcccccct cggatcgggc ccgtggcgca ggacctgctg cagcggctgc 901 tttgtaagga tcctaagaag cgattgggcg cggggcccca gggggcacaa gaagtccgga 961 accatccctt cttccagggc ctcgattggg tggctctggc tgccaggaag attccagccc 1021 cattccggcc ccaaatccgc tcagagctgg atgtgggcaa ctttgcggag gaattcactc 1081 ggctggagcc tgtctactca ccccctggca gccccccacc tggggacccc cgaatctttc 1141 agggatactc ctttgtggca ccctccattc tctttgacca caacaacgcg gtgatgaccg 1201 atgggctgga agcgcctggt gctggagacc ggccaggtcg ggcagcggtg gccaggagcg 1261 ctatgatgca ggactcgccc ttcttccagc agtacgagct ggacctgcgg gagcctgcgc 1321 tgggccaggg cagcttttct gtgtgtcgcc gctgctgcca gcgccagagc ggccaggagt 1381 tcgcagtcaa gatcctcagt cgcaggctgg aggcgaacac gcagcgcgaa gtggctgccc 1441 tgcgcctgtg ccagtcacac cccaacgtgg tgaatctgca cgaggtgcat cacgaccagc 1501 tgcacacgta cctggtcctg gagctgctgc ggggcgggga gctgctggag cacatccgca 1561 agaagcggca cttcagcgag tcggaagcaa gccagatcct gcgcagcctc gtgtcggccg 1621 tgagcttcat gcacgaggag gcgggcgtgg tgcaccgcga cctcaagccg gagaacatcc 1681 tgtacgccga cgacacgccc ggggccccgg tgaaaatcat cgacttcggg ttcgcgcggt 1741 tgcggccgca gagtcccggg gtgcccatgc agacgccctg cttcacgctg cagtacgctg 1801 cccccgagct gctggcgcag cagggctacg acgagtcctg cgacctctgg agcctgggcg 1861 tcattctgta catgatgctg tcggggcagg tccccttcca gggggcctct ggccagggcg 1921 ggcagagcca ggcggccgag atcatgtgca aaatccgcga ggggcgcttc tcccttgacg 1981 gggaggcctg gcagggtgta tccgaggaag ccaaggagct ggTCCGAGGG CTCCTGACCG 2041 TGGACCCCGC CAAGCGGCTG AAGCTCGAGG GACTGCGGGG CAGCTCGTGG CTGCAGGACG 2101 GCAGCGCGCG CTCCTCGCCC CCGCTCCGGA CGCCCGACGT GCTCGAGTCC TCTGGGCCCG 2161 CAGTGCGCTC GGGTCTCAAC GCCACCTTCA TGGCATTCAA CCGGGGCAAG CGGGAGGGCT 2221 TCTTCCTGAA GAGCGTGGAG AATGCACCCC TGGCCAAGCG GCGGAAGCAG AAGCTGCGGA 2281 GCGCCACCGC CTCCCGCCGG GGCTCCCCTG CACCAGCCAA CCCGGGCCGA GCCCCCGTCG 2341 CCTCCAAAGG GGCCCCCCGC CGAGCCAACG GCCCCCTGCC CCCCTCCTAG GACCCAGCTT 2401 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2461 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2521 CTTGTGGAAA GGACGAATCC TCGGACATCT AGAGCCCTCG ACGCGTTAAG TCgacaatca 2581 acctctggat tacaaaattt gtgaaagatt