Construct: ORF TRCN0000489077
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020314.1_s317c1
- DNA Barcode:
- ATGGGGTGCATTTCCAGAGTCGCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TGFBR2 (7048)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489077
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7048 | TGFBR2 | transforming growth factor ... | NM_003242.6 | 100% | 100% | |
| 2 | human | 7048 | TGFBR2 | transforming growth factor ... | NM_001024847.2 | 95.7% | 95.6% | 95_169del |
| 3 | human | 7048 | TGFBR2 | transforming growth factor ... | XM_011534043.2 | 94.5% | 91.3% | (many diffs) |
| 4 | human | 7048 | TGFBR2 | transforming growth factor ... | XM_011534045.3 | 93.8% | 93.8% | 0_1ins105 |
| 5 | human | 7048 | TGFBR2 | transforming growth factor ... | XM_017007106.1 | 93.8% | 93.8% | 0_1ins105 |
| 6 | mouse | 21813 | Tgfbr2 | transforming growth factor,... | NM_029575.3 | 87.2% | 92.2% | (many diffs) |
| 7 | mouse | 21813 | Tgfbr2 | transforming growth factor,... | NM_009371.3 | 84.1% | 88.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1773
- ORF length:
- 1701
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggtcgg gggctgctca ggggcctgtg gccgctgcac atcgtcctgt 121 ggacgcgtat cgccagcacg atcccaccgc acgttcagaa gtcggttaat aacgacatga 181 tagtcactga caacaacggt gcagtcaagt ttccacaact gtgtaaattt tgtgatgtga 241 gattttccac ctgtgacaac cagaaatcct gcatgagcaa ctgcagcatc acctccatct 301 gtgagaagcc acaggaagtc tgtgtggctg tatggagaaa gaatgacgag aacataacac 361 tagagacagt ttgccatgac cccaagctcc cctaccatga ctttattctg gaagatgctg 421 cttctccaaa gtgcattatg aaggaaaaaa aaaagcctgg tgagactttc ttcatgtgtt 481 cctgtagctc tgatgagtgc aatgacaaca tcatcttctc agaagaatat aacaccagca 541 atcctgactt gttgctagtc atatttcaag tgacaggcat cagcctcctg ccaccactgg 601 gagttgccat atctgtcatc atcatcttct actgctaccg cgttaaccgg cagcagaagc 661 tgagttcaac ctgggaaacc ggcaagacgc ggaagctcat ggagttcagc gagcactgtg 721 ccatcatcct ggaagatgac cgctctgaca tcagctccac gtgtgccaac aacatcaacc 781 acaacacaga gctgctgccc attgagctgg acaccctggt ggggaaaggt cgctttgctg 841 aggtctataa ggccaagctg aagcagaaca cttcagagca gtttgagaca gtggcagtca 901 agatctttcc ctatgaggag tatgcctctt ggaagacaga gaaggacatc ttctcagaca 961 tcaatctgaa gcatgagaac atactccagt tcctgacggc tgaggagcgg aagacggagt 1021 tggggaaaca atactggctg atcaccgcct tccacgccaa gggcaaccta caggagtacc 1081 tgacgcggca tgtcatcagc tgggaggacc tgcgcaagct gggcagctcc ctcgcccggg 1141 ggattgctca cctccacagt gatcacactc catgtgggag gcccaagatg cccatcgtgc 1201 acagggacct caagagctcc aatatcctcg tgaagaacga cctaacctgc tgcctgtgtg 1261 actttgggct ttccctgcgt ctggacccta ctctgtctgt ggatgacctg gctaacagtg 1321 ggcaggtggg aactgcaaga tacatggctc cagaagtcct agaatccagg atgaatttgg 1381 agaatgttga gtccttcaag cagaccgatg tctactccat ggcTCTGGTG CTCTGGGAAA 1441 TGACATCTCG CTGTAATGCA GTGGGAGAAG TAAAAGATTA TGAGCCTCCA TTTGGTTCCA 1501 AGGTGCGGGA GCACCCCTGT GTCGAAAGCA TGAAGGACAA CGTGTTGAGA GATCGAGGGC 1561 GACCAGAAAT TCCCAGCTTC TGGCTCAACC ACCAGGGCAT CCAGATGGTG TGTGAGACGT 1621 TGACTGAGTG CTGGGACCAC GACCCAGAGG CCCGTCTCAC AGCCCAGTGT GTGGCAGAAC 1681 GCTTCAGTGA GCTGGAGCAT CTGGACAGGC TCTCGGGGAG GAGCTGCTCG GAGGAGAAGA 1741 TTCCTGAAGA CGGCTCCCTA AACACTACCA AATGAGACCC AGCTTTCTTG TACAAAGTGG 1801 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1861 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1921 AATGGGGTGC ATTTCCAGAG TCGCAACGCG TTAAGTCgac aatcaacctc tggattacaa 1981 aatttgtgaa agatt