Construct: ORF TRCN0000489084
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019192.1_s317c1
- DNA Barcode:
- TTTAACCCATGTCCATTTAATTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TAS2R31 (259290)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489084
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 259290 | TAS2R31 | taste 2 receptor member 31 | NM_176885.2 | 100% | 100% | |
| 2 | human | 259289 | TAS2R43 | taste 2 receptor member 43 | NM_176884.2 | 94.7% | 89.3% | (many diffs) |
| 3 | human | 259292 | TAS2R46 | taste 2 receptor member 46 | NM_176887.2 | 92.4% | 85.1% | (many diffs) |
| 4 | human | 259291 | TAS2R45 | taste 2 receptor member 45 | NM_176886.2 | 89.3% | 81.5% | (many diffs) |
| 5 | human | 259289 | TAS2R43 | taste 2 receptor member 43 | XM_003960991.5 | 89.3% | 81.5% | (many diffs) |
| 6 | human | 259293 | TAS2R30 | taste 2 receptor member 30 | NM_001097643.1 | 88.9% | 77.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1002
- ORF length:
- 927
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgaca acttttatac ccatcatttt ttccagtgtg gtagtggttc 121 tatttgttat tggaaatttt gctaatggct tcatagcatt ggtaaattcc attgagcggg 181 tcaagagaca aaagatctct tttgctgacc agattctcac tgctctggcg gtctccagag 241 ttggtttgct ctgggtatta ttattaaatt ggtattcaac tgtgtttaat ccagcttttt 301 atagtgtaga agtaagaact actgcttata atgtctgggc agtaaccggc catttcagca 361 actggcttgc tactagcctc agcatatttt atttgctcaa gattgccaat ttctccaacc 421 ttatttttct tcacttaaag aggagagtta agagtgtcat tctggtgatg ctgttggggc 481 ctttactatt tttggcttgt caactttttg tgataaacat gaaagagatt gtacggacaa 541 aagaatatga aggaaacttg acttggaaga tcaaattgag gagtgcagtg tacctttcag 601 atgcgactgt aaccacgcta ggaaacttag tgcccttcac tctgacccTG CTATGTTTTT 661 TGCTGTTAAT CTGTTCTCTG TGTAAACATC TCAAGAAGAT GCAGCTCCAT GGTAAAGGAT 721 CTCAAGATCC CAGCACCAAG GTCCACATAA AAGCTTTGCA AACTGTGATC TTTTTCCTCT 781 TGTTATGTGC CGTTTACTTT CTGTCCATAA TGATATCAGT TTGGAGTTTT GGGAGTCTGG 841 AAAACAAACC TGTCTTCATG TTCTGCAAAG CTATTAGATT CAGCTATCCT TCAATCCACC 901 CATTCATCCT GATTTGGGGA AACAAGAAGC TAAAGCAGAC TTTTCTTTCA GTTTTGCGGC 961 AAGTGAGGTA CTGGGTGAAA GGAGAGAAGC CTTCATCTCC AGACCCAGCT TTCTTGTACA 1021 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1081 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1141 AGGACGATTT AACCCATGTC CATTTAATTG GACGCGTTAA GTCgacaatc aacctctgga 1201 ttacaaaatt tgtgaaagat t