Construct: ORF TRCN0000489089
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020115.1_s317c1
- DNA Barcode:
- TAAAATGGTCTTGATCTTCCTAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC22A6 (9356)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489089
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 30811 | HUNK | hormonally up-regulated Neu... | NM_014586.2 | 99.8% | 99.8% | 1413T>C;1548T>C;2142_2143insG |
| 2 | human | 30811 | HUNK | hormonally up-regulated Neu... | XM_011529537.2 | 97.6% | 97.6% | (many diffs) |
| 3 | mouse | 26559 | Hunk | hormonally upregulated Neu-... | NM_015755.2 | 87.2% | 92.3% | (many diffs) |
| 4 | mouse | 26559 | Hunk | hormonally upregulated Neu-... | XM_011246130.2 | 78.4% | 80% | (many diffs) |
| 5 | mouse | 26559 | Hunk | hormonally upregulated Neu-... | XR_384941.2 | 46.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2214
- ORF length:
- 2145
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gccggcggcg gcgggggacg ggctcctggg ggagccggcg gcgcctgggg 121 gcggcggcgg cgcggaggac gcggccaggc ccgcggcggc ctgcgaggga agtttcctgc 181 ctgcctgggt gagcggcgtg ccccgcgagc ggctccgcga cttccagcac cacaagcgcg 241 tgggcaacta cctcatcggc agcaggaagc tgggcgaggg ctcctttgcc aaggtgcgcg 301 aggggctgca cgtgctgacc ggggagaagg tggccataaa agtcattgat aagaagagag 361 ccaaaaagga cacctatgtc accaaaaacc tgcggcgaga gggtcagatc cagcagatga 421 tccgccaccc caatatcact cagctccttg atattttaga aacggaaaac agctactacc 481 tggtcatgga gctgtgccct gggggcaacc tgatgcacaa gatctatgag aagaagcggc 541 tggaggagtc cgaagcccgc agatacatcc gacagctcat ctctgccgta gagcacctgc 601 accgggccgg ggtggtccac agagacttga agatagagaa tttgctacta gatgaagaca 661 ataatatcaa gctgattgac tttggtttga gcaactgcgc agggatcctg ggttactcgg 721 atccgttcag cacacagtgt ggcagccctg cctacgctgc acctgaactg ctcgccagga 781 agaaatacgg ccccaaaatc gatgtctggt ccataggtgt gaacatgtat gccatgttga 841 ccgggacgct gcctttcacg gtggagcctt tcagcctgag ggctttgtac cagaagatgg 901 tagacaaaga aatgaacccc ctccccactc agctctccac aggtgccatc agtttcctgc 961 gctctctcct ggaaccggat cctgtgaaga ggccaaatat tcagcaggca ctggcgaatc 1021 gctggcttaa tgagaattac acgggcaaag tgccctgtaa tgtcacctat cccaacagga 1081 tttctctgga agatctgagc ccgagcgtcg tgctgcacat gaccgagaag ctgggttaca 1141 agaacagcga cgtgatcaac actgtgctct ccaaccgcgc ctgccacatc ctggccatct 1201 acttcctctt aaacaagaaa ctggagcgct atttgtcagg gaaatctgac atccaggaca 1261 gcctctgcta caagacccgg ctctaccaga tagaaaagta cagggccccc aaggagtcct 1321 atgaggcctc tctggacacc tggacacgag atcttgaatt ccatgccgtg caggataaaa 1381 agcccaaaga acaagaaaaa agaggggatt ttcttcatcg accattctcc aagaagttgg 1441 acaagaacct gccctcgcac aaacagccct caggctcgct catgacacag attcagaaca 1501 ccaaagccct cctgaaggac cggaaggcct ccaagtccag cttccccgac aaagattcct 1561 ttggctgccg caatattttc cgcaaaacct cagattccaa ttgtgtggct tcttcctcca 1621 tggagttcat ccccgtgcca ccgcccagga ccccgaggat tgtgaagaaa ccggagcccc 1681 atcagccagg gcccggaagc actggcatcc cccacaagga agaccccctg atgctggaca 1741 tggtgcgctc cttcgagtct gtggatcgcg acgaccacgt agaagtgctg tctccctctc 1801 atcactacag gattctgaac tccccggtca gcttggctcg cagaaattcc agcgagagga 1861 cgctgtcccc GGGTCTGCCA TCCGGAAGCA TGTCGCCTCT CCATACTCCT TTGCATCCAA 1921 CTCTGGTCTC TTTTGCTCAC GAAGATAAGA ACAGCCCCCC AAAAGAGGAG GGCCTGTGTT 1981 GCCCACCTCC GGTTCCCAGC AATGGCCCCA TGCAGCCTCT GGGGAGCCCC AATTGTGTGA 2041 AAAGCCGAGG CCGGTTCCCT ATGATGGGCA TCGGACAGAT GTTAAGGAAG CGCCATCAGA 2101 GTCTGCAGCC ATCTGCAGAT AGGCCCCTGG AGGCCAGCCT GCCCCCACTG CAGCCCCTAG 2161 CCCCTGTGAA CCTTGCCTTT GACATGGCCG ATGGGGTCAA GACCCAGTGC GACCCAGCTT 2221 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2281 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2341 CTTGTGGAAA GGACGATAAA ATGGTCTTGA TCTTCCTAAC ACGCGTTAAG TCgacaatca 2401 acctctggat tacaaaattt gtgaaagatt