Construct: ORF TRCN0000489104
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021108.1_s317c1
- DNA Barcode:
- AGAATAAGACATAGAATAAACCCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- LGR5 (8549)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489104
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8549 | LGR5 | leucine rich repeat contain... | NM_003667.4 | 99.9% | 100% | 882G>A |
| 2 | human | 8549 | LGR5 | leucine rich repeat contain... | NM_001277226.2 | 97.3% | 97.3% | 784_785ins72;810G>A |
| 3 | human | 8549 | LGR5 | leucine rich repeat contain... | NM_001277227.2 | 92% | 92% | 426_427ins216;666G>A |
| 4 | human | 8549 | LGR5 | leucine rich repeat contain... | NR_110596.2 | 43.9% | (many diffs) | |
| 5 | mouse | 14160 | Lgr5 | leucine rich repeat contain... | NM_010195.2 | 84.6% | 85.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2793
- ORF length:
- 2721
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggacacc tcccggctcg gtgtgctcct gtccttgcct gtgctgctgc 121 agctggcgac cgggggcagc tctcccaggt ctggtgtgtt gctgaggggc tgccccacac 181 actgtcattg cgagcccgac ggcaggatgt tgctcagggt ggactgctcc gacctggggc 241 tctcggagct gccttccaac ctcagcgtct tcacctccta cctagacctc agtatgaaca 301 acatcagtca gctgctcccg aatcccctgc ccagtctccg cttcctggag gagttacgtc 361 ttgcgggaaa cgctctgaca tacattccca agggagcatt cactggcctt tacagtctta 421 aagttcttat gctgcagaat aatcagctaa gacacgtacc cacagaagct ctgcagaatt 481 tgcgaagcct tcaatccctg cgtctggatg ctaaccacat cagctatgtg cccccaagct 541 gtttcagtgg cctgcattcc ctgaggcacc tgtggctgga tgacaatgcg ttaacagaaa 601 tccccgtcca ggcttttaga agtttatcgg cattgcaagc catgaccttg gccctgaaca 661 aaatacacca cataccagac tatgcctttg gaaacctctc cagcttggta gttctacatc 721 tccataacaa tagaatccac tccctgggaa agaaatgctt tgatgggctc cacagcctag 781 agactttaga tttaaattac aataaccttg atgaattccc cactgcaatt aggacactct 841 ccaaccttaa agaactagga tttcatagca acaatatcag gtcgatacct gagaaagcat 901 ttgtaggcaa cccttctctt attacaatac atttctatga caatcccatc caatttgttg 961 ggagatctgc ttttcaacat ttacctgaac taagaacact gactctgaat ggtgcctcac 1021 aaataactga atttcctgat ttaactggaa ctgcaaacct ggagagtctg actttaactg 1081 gagcacagat ctcatctctt cctcaaaccg tctgcaatca gttacctaat ctccaagtgc 1141 tagatctgtc ttacaaccta ttagaagatt tacccagttt ttcagtctgc caaaagcttc 1201 agaaaattga cctaagacat aatgaaatct acgaaattaa agttgacact ttccagcagt 1261 tgcttagcct ccgatcgctg aatttggctt ggaacaaaat tgctattatt caccccaatg 1321 cattttccac tttgccatcc ctaataaagc tggacctatc gtccaacctc ctgtcgtctt 1381 ttcctataac tgggttacat ggtttaactc acttaaaatt aacaggaaat catgccttac 1441 agagcttgat atcatctgaa aactttccag aactcaaggt tatagaaatg ccttatgctt 1501 accagtgctg tgcatttgga gtgtgtgaga atgcctataa gatttctaat caatggaata 1561 aaggtgacaa cagcagtatg gacgaccttc ataagaaaga tgctggaatg tttcaggctc 1621 aagatgaacg tgaccttgaa gatttcctgc ttgactttga ggaagacctg aaagcccttc 1681 attcagtgca gtgttcacct tccccaggcc ccttcaaacc ctgtgaacac ctgcttgatg 1741 gctggctgat cagaattgga gtgtggacca tagcagttct ggcacttact tgtaatgctt 1801 tggtgacttc aacagttttc agatcccctc tgtacatttc ccccattaaa ctgttaattg 1861 gggtcatcgc agcagtgaac atgctcacgg gagtctccag tgccgtgctg gctggtgtgg 1921 atgcgttcac ttttggcagc tttgcacgac atggtgcctg gtgggagaat ggggttggtt 1981 gccatgtcat tggttttttg tccatttttg cttcagaatc atctgttttc ctgcttactc 2041 tggcagccct ggagcgtggg ttctctgtga aatattctgc aaaatttgaa acgaaagctc 2101 cattttctag cctgaaagta atcattttgc tctgtgccct gctggccttg accatggccg 2161 cagttcccct gctgggtggc agcaagtatg gcgcctcccc tctctgcctg cctttgcctt 2221 ttggggagcc cagcaccatg ggctacatgg tcgctctcat cttgctcaat tccctttgct 2281 tcctcatgat gaccattgcc tacaccaagc tctactgcaa tttggacaag ggagacctgg 2341 agaatatttg ggactgctct atggtaaaac acattgccct gttgctcttc accaactgca 2401 tcctaaactg ccctgtggct ttcttgtccT TCTCCTCTTT AATAAACCTT ACATTTATCA 2461 GTCCTGAAGT AATTAAGTTT ATCCTTCTGG TGGTAGTCCC ACTTCCTGCA TGTCTCAATC 2521 CCCTTCTCTA CATCTTGTTC AATCCTCACT TTAAGGAGGA TCTGGTGAGC CTGAGAAAGC 2581 AAACCTACGT CTGGACAAGA TCAAAACACC CAAGCTTGAT GTCAATTAAC TCTGATGATG 2641 TCGAAAAACA GTCCTGTGAC TCAACTCAAG CCTTGGTAAC CTTTACCAGC TCCAGCATCA 2701 CTTATGACCT GCCTCCCAGT TCCGTGCCAT CACCAGCTTA TCCAGTGACT GAGAGCTGCC 2761 ATCTTTCCTC TGTGGCATTT GTCCCATGTC TCTAAGACCC AGCTTTCTTG TACAAAGTGG 2821 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2881 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2941 AAGAATAAGA CATAGAATAA ACCCGACGCG TTAAGTCgac aatcaacctc tggattacaa 3001 aatttgtgaa agatt