Construct: ORF TRCN0000489106
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021081.1_s317c1
- DNA Barcode:
- ATACTAGGCTGGCTGGTCGGCCTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PTH2R (5746)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489106
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5746 | PTH2R | parathyroid hormone 2 receptor | NM_005048.4 | 100% | 100% | |
| 2 | human | 5746 | PTH2R | parathyroid hormone 2 receptor | NM_001309516.2 | 79.8% | 79.8% | 0_1ins333 |
| 3 | human | 5746 | PTH2R | parathyroid hormone 2 receptor | NM_001371905.1 | 79.8% | 79.8% | 0_1ins333 |
| 4 | human | 5746 | PTH2R | parathyroid hormone 2 receptor | NM_001371906.1 | 79.8% | 79.8% | 0_1ins333 |
| 5 | human | 5746 | PTH2R | parathyroid hormone 2 receptor | NM_001371907.1 | 63% | 63% | 0_1ins333;647_648ins276 |
| 6 | human | 5746 | PTH2R | parathyroid hormone 2 receptor | NR_163993.1 | 53.1% | (many diffs) | |
| 7 | human | 5746 | PTH2R | parathyroid hormone 2 receptor | NR_163994.1 | 52.6% | (many diffs) | |
| 8 | human | 5746 | PTH2R | parathyroid hormone 2 receptor | NR_163991.1 | 51.8% | (many diffs) | |
| 9 | human | 5746 | PTH2R | parathyroid hormone 2 receptor | NR_163992.1 | 50.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1722
- ORF length:
- 1650
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccggg ctgggggcgt cgctccacgt ctggggttgg ctaatgctcg 121 gcagctgcct cctggccaga gcccagctgg attctgatgg caccattact atagaggagc 181 agattgtcct tgtgctgaaa gcgaaagtac aatgtgaact caacatcaca gctcaactcc 241 aggagggaga aggtaattgt ttccctgaat gggatggact catttgttgg cccagaggaa 301 cagtggggaa aatatcggct gttccatgcc ctccttatat ttatgacttc aaccataaag 361 gagttgcttt ccgacactgt aaccccaatg gaacatggga ttttatgcac agcttaaata 421 aaacatgggc caattattca gactgccttc gctttctgca gccagatatc agcataggaa 481 agcaagaatt ctttgaacgc ctctatgtaa tgtataccgt tggctactcc atctcttttg 541 gttccttggc tgtggctatt ctcatcattg gttacttcag acgattgcat tgcactagga 601 actatatcca catgcactta tttgtgtctt tcatgctgag agctacaagc atctttgtca 661 aagacagagt agtccatgct cacataggag taaaggagct ggagtcccta ataatgcagg 721 atgacccaca aaattccatt gaggcaactt ctgtggacaa atcacaatat atcgggtgca 781 agattgctgt tgtgatgttt atttacttcc tggctacaaa ttattattgg atcctggtgg 841 aaggtctcta cctgcataat ctcatctttg tggctttctt ttcggacacc aaatacctgt 901 ggggcttcat cttgataggc tgggggtttc cagcagcatt tgttgcagca tgggctgtgg 961 cacgagcaac tctggctgat gcgaggtgct gggaacttag tgctggagac atcaagtgga 1021 tttatcaagc accgatctta gcagctattg ggctgaattt tattctgttt ctgaatacgg 1081 ttagagttct agctaccaaa atctgggaga ccaatgcagt tgggcatgac acaaggaagc 1141 aatacaggaa actggccaaa tcgacactgg tcctggtcct agtctttgga gtgcattaca 1201 tcgtgttcgt atgcctgcct cactccttca ctgggctcgg gtgggagatc cgcatgcact 1261 gtgagctctt cttcaactcc tttcagggtt tctttgtgtc tatcatctac tgctactgca 1321 atGGAGAGGT TCAGGCAGAG GTGAAGAAGA TGTGGAGTCG GTGGAACCTC TCCGTGGACT 1381 GGAAAAGGAC ACCGCCATGT GGCAGCCGCA GATGCGGCTC AGTGCTCACC ACCGTGACGC 1441 ACAGCACCAG CAGCCAGTCA CAGGTGGCGG CCAGCACACG CATGGTGCTT ATCTCTGGCA 1501 AAGCTGCCAA GATCGCCAGC AGACAGCCTG ACAGCCACAT CACTTTACCT GGCTATGTCT 1561 GGAGTAACTC AGAGCAGGAC TGCCTGCCAC ACTCTTTCCA CGAGGAGACC AAGGAAGATA 1621 GTGGGAGGCA GGGAGATGAT ATTCTAATGG AGAAGCCTTC CAGGCCTATG GAATCTAACC 1681 CAGACACTGA AGGATGCCAA GGAGAAACTG AGGATGTTCT CTGAGACCCA GCTTTCTTGT 1741 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1801 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1861 GAAAGGACGA ATACTAGGCT GGCTGGTCGG CCTCACGCGT TAAGTCgaca atcaacctct 1921 ggattacaaa atttgtgaaa gatt