Construct: ORF TRCN0000489107
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021017.1_s317c1
- DNA Barcode:
- TTCTTTCATCGTCTTCAACTGTAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CRHR2 (1395)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489107
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1395 | CRHR2 | corticotropin releasing hor... | NM_001883.5 | 100% | 100% | |
| 2 | human | 1395 | CRHR2 | corticotropin releasing hor... | XM_024446665.1 | 100% | 100% | |
| 3 | human | 1395 | CRHR2 | corticotropin releasing hor... | NM_001202482.1 | 99.7% | 99.7% | 313_314insAGC |
| 4 | human | 1395 | CRHR2 | corticotropin releasing hor... | NM_001202481.1 | 93.2% | 90.9% | (many diffs) |
| 5 | human | 1395 | CRHR2 | corticotropin releasing hor... | XM_017011752.2 | 93.2% | 90.9% | (many diffs) |
| 6 | human | 1395 | CRHR2 | corticotropin releasing hor... | NM_001202483.1 | 92.6% | 86.7% | (many diffs) |
| 7 | human | 1395 | CRHR2 | corticotropin releasing hor... | NM_001202475.1 | 91.3% | 85.5% | (many diffs) |
| 8 | mouse | 12922 | Crhr2 | corticotropin releasing hor... | NM_001288619.1 | 88.6% | 92.7% | (many diffs) |
| 9 | mouse | 12922 | Crhr2 | corticotropin releasing hor... | XM_006505482.3 | 88.4% | 92.4% | (many diffs) |
| 10 | mouse | 12922 | Crhr2 | corticotropin releasing hor... | NM_001288618.1 | 82.3% | 80.3% | (many diffs) |
| 11 | mouse | 12922 | Crhr2 | corticotropin releasing hor... | NM_009953.4 | 82.1% | 80.1% | (many diffs) |
| 12 | mouse | 12922 | Crhr2 | corticotropin releasing hor... | NM_001288620.1 | 66% | 58.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1305
- ORF length:
- 1233
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggacgcg gcactgctcc acagcctgct ggaggccaac tgcagcctgg 121 cgctggctga agagctgctc ttggacggct gggggccacc cctggacccc gagggtccct 181 actcctactg caacacgacc ttggaccaga tcggaacgtg ctggccccgc agcgctgccg 241 gagccctcgt ggagaggccg tgccccgagt acttcaacgg cgtcaagtac aacacgaccc 301 ggaatgccta tcgagaatgc ttggagaatg ggacgtgggc ctcaaagatc aactactcac 361 agtgtgagcc cattttggat gacaagcaga ggaagtatga cctgcactac cgcatcgccc 421 ttgtcgtcaa ctacctgggc cactgcgtat ctgtggcagc cctggtggcc gccttcctgc 481 ttttcctggc cctgcggagc attcgctgtc tgcggaatgt gattcactgg aacctcatca 541 ccacctttat cctgcgaaat gtcatgtggt tcctgctgca gctcgttgac catgaagtgc 601 acgagagcaa tgaggtctgg tgccgctgca tcaccaccat cttcaactac ttcgtggtga 661 ccaacttctt ctggatgttt gtggaaggct gctacctgca cacggccatt gtcatgacct 721 actccactga gcgcctgcgc aagtgcctct tcctcttcat cggatggtgc atccccttcc 781 ccatcatcgt cgcctgggcc atcggcaagc tctactatga gaatgaacag tgctggtttg 841 gcaaggagcc tggcgacctg gtggactaca tctaccaagg ccccatcatt ctcgtgctcc 901 tgatcaattt cgtatttctg ttcaacatcg tcaggaTCCT AATGACAAAG TTACGCGCGT 961 CCACCACATC CGAGACAATC CAGTACAGGA AGGCAGTGAA GGCCACCCTG GTGCTCCTGC 1021 CCCTCCTGGG CATCACCTAC ATGCTCTTCT TCGTCAATCC CGGGGAGGAC GACCTGTCAC 1081 AGATCATGTT CATCTATTTC AACTCCTTCC TGCAGTCGTT CCAGGGTTTC TTCGTGTCTG 1141 TCTTCTACTG CTTCTTCAAT GGAGAGGTGC GCTCAGCCGT GAGGAAGAGG TGGCACCGCT 1201 GGCAGGACCA TCACTCCCTT CGAGTCCCCA TGGCCCGGGC CATGTCCATC CCTACATCAC 1261 CCACACGGAT CAGCTTCCAC AGCATCAAGC AGACGGCCGC TGTGTAGAAC CCAGCTTTCT 1321 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1381 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1441 GTGGAAAGGA CGATTCTTTC ATCGTCTTCA ACTGTAGACG CGTTAAGTCg acaatcaacc 1501 tctggattac aaaatttgtg aaagatt