Construct: ORF TRCN0000489111
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020847.1_s317c1
- DNA Barcode:
- TTGCTCAACAGTAGGATTATGTCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HTR1E (3354)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489111
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3354 | HTR1E | 5-hydroxytryptamine recepto... | NM_000865.3 | 100% | 100% | |
| 2 | human | 3354 | HTR1E | 5-hydroxytryptamine recepto... | XM_011535789.2 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1167
- ORF length:
- 1095
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaacatc acaaactgta ccacagaggc cagcatggct ataagaccca 121 agaccatcac tgagaagatg ctcatttgca tgactctggt ggtcatcacc accctcacca 181 cgttgctgaa cttggctgtg atcatggcta ttggcaccac caagaagctc caccagcctg 241 ccaactacct aatctgttct ctggccgtga cggacctcct ggtggcagtg ctcgtcatgc 301 ccctgagcat catctacatt gtcatggatc gctggaagct tgggtacttc ctctgtgagg 361 tgtggctgag tgtggacatg acctgctgca cctgctccat cctccacctc tgtgtcattg 421 ccctggacag gtactgggcc atcaccaatg ctattgaata cgccaggaag aggacggcca 481 agagggccgc gctgatgatc cttaccgtct ggaccatctc cattttcatc tccatgcccc 541 ctctgttctg gagaagccac cgccgcctaa gccctccccc tagtcagtgc accatccagc 601 acgaccatgt tatctacacc atttactcca cgctgggtgc gttttatatc cccttgactt 661 tgatactgat tctctattac cggatttacc acgcggccaa gagcctttac cagaaaaggg 721 gatcaagtcg gcacttaagc aacagaagca cagatagcca gaattctttt gcaagttgta 781 aacttacaca gactttctgt gtgtctgact tctccacctc agaccctacc acagagtttg 841 aaaagttcca tgcctccatc aggatccccc ccttcgacaa tgatctagaT CACCCAGGAG 901 AACGTCAGCA GATCTCTAGC ACCAGGGAAC GGAAGGCAGC ACGCATCCTG GGGCTGATTC 961 TGGGTGCATT CATTTTATCC TGGCTGCCAT TTTTCATCAA AGAGTTGATT GTGGGTCTGA 1021 GCATCTACAC CGTGTCCTCG GAAGTGGCCG ACTTTCTGAC GTGGCTCGGT TATGTGAATT 1081 CTCTGATCAA CCCTCTGCTC TATACGAGTT TTAATGAAGA CTTTAAGCTG GCTTTTAAAA 1141 AGCTCATTAG ATGCCGAGAG CATACTTAGG ACCCAGCTTT CTTGTACAAA GTGGTTGATA 1201 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1261 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATTGCT 1321 CAACAGTAGG ATTATGTCCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1381 tgaaagatt