Construct: ORF TRCN0000489117

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020512.1_s317c1
DNA Barcode:
AATACTATCCATGATCAAATACAC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
DDR2 (4921)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489117
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4921 DDR2 discoidin domain receptor t... NM_001014796.3 99.9% 100% 1260C>G
2 human 4921 DDR2 discoidin domain receptor t... NM_001354982.2 99.9% 100% 1260C>G
3 human 4921 DDR2 discoidin domain receptor t... NM_001354983.2 99.9% 100% 1260C>G
4 human 4921 DDR2 discoidin domain receptor t... NM_006182.4 99.9% 100% 1260C>G
5 human 4921 DDR2 discoidin domain receptor t... XM_011509587.2 99.9% 100% 1260C>G
6 human 4921 DDR2 discoidin domain receptor t... XM_011509588.3 62.8% 58.8% (many diffs)
7 mouse 18214 Ddr2 discoidin domain receptor f... NM_022563.2 90.2% 95.9% (many diffs)
8 mouse 18214 Ddr2 discoidin domain receptor f... XM_006496696.3 90.2% 95.9% (many diffs)
9 mouse 18214 Ddr2 discoidin domain receptor f... XM_006496697.3 90.2% 95.9% (many diffs)
10 mouse 18214 Ddr2 discoidin domain receptor f... XM_006496698.3 90.2% 95.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
2637
ORF length:
2565
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgatcctg attcccagaa tgctcttggt gctgttcctg ctgctgccta
121tcttgagttc tgcaaaagct caggttaatc cagctatatg ccgctatcct ctgggcatgt
181caggaggcca gattccagat gaggacatca cagcttccag tcagtggtca gagtccacag
241ctgccaaata tggaaggctg gactcagaag aaggggatgg agcctggtgc cctgagattc
301cagtggaacc tgatgacctg aaggagtttc tgcagattga cttgcacacc ctccatttta
361tcactctggt ggggacccag gggcgccatg caggaggtca tggcatcgag tttgccccca
421tgtacaagat caattacagt cgggatggca ctcgctggat ctcttggcgg aaccgtcatg
481ggaaacaggt gctggatgga aatagtaacc cctatgacat tttcctaaag gacttggagc
541cgcccattgt agccagattt gtccggttca ttccagtcac cgaccactcc atgaatgtgt
601gtatgagagt ggagctttac ggctgtgtct ggctagatgg cttggtgtct tacaatgctc
661cagctgggca gcagtttgta ctccctggag gttccatcat ttatctgaat gattctgtct
721atgatggagc tgttggatac agcatgacag aagggctagg ccaattgacc gatggtgtgt
781ctggcctgga cgatttcacc cagacccatg aataccacgt gtggcccggc tatgactatg
841tgggctggcg gaacgagagt gccaccaatg gctacattga gatcatgttt gaatttgacc
901gcatcaggaa tttcactacc atgaaggtcc actgcaacaa catgtttgct aaaggtgtga
961agatctttaa ggaggtacag tgctacttcc gctctgaagc cagtgagtgg gaacctaatg
1021ccatttcctt cccccttgtc ctggatgacg tcaaccccag tgctcggttt gtcacggtgc
1081ctctccacca ccgaatggcc agtgccatca agtgtcaata ccattttgca gatacctgga
1141tgatgttcag tgagatcacc ttccaatcag atgctgcaat gtacaacaac tctgaagccc
1201tgcccacctc tcctatggca cccacaacct atgatccaat gcttaaagtt gatgacagca
1261acactcggat cctgattggc tgcttggtgg ccatcatctt tatcctcctg gccatcattg
1321tcatcatcct gtggaggcag ttctggcaga aaatgctgga gaaggcttct cggaggatgc
1381tggatgatga aatgacagtc agcctttccc tgccaagtga ttctagcatg ttcaacaata
1441accgctcctc atcacctagt gaacaagggt ccaactcgac ttacgatcgc atctttcccc
1501ttcgccctga ctaccaggag ccatccaggc tgatacgaaa actcccagaa tttgctccag
1561gggaggagga gtcaggctgc agcggtgttg tgaagccagt ccagcccagt ggccctgagg
1621gggtgcccca ctatgcagag gctgacatag tgaacctcca aggagtgaca ggaggcaaca
1681catactcagt gcctgccgtc accatggacc tgctctcagg aaaagatgtg gctgtggagg
1741agttccccag gaaactccta actttcaaag agaagctggg agaaggacag tttggggagg
1801ttcatctctg tgaagtggag ggaatggaaa aattcaaaga caaagatttt gccctagatg
1861tcagtgccaa ccagcctgtc ctggtggctg tgaaaatgct ccgagcagat gccaacaaga
1921atgccaggaa tgattttctt aaggagataa agatcatgtc tcggctcaag gacccaaaca
1981tcatccatct attagctgtg tgtatcactg atgaccctct ctgtatgatc actgaataca
2041tggagaatgg agatctcaat cagtttcttt cccgccacga gccccctaat tcttcctcca
2101gcgatgtacg cactgtcagt tacaccaatc tgaagtttat ggctacccaa attgcctctg
2161gcatgaagta cctttcctct cttaattttg ttcaccgaga tctggccaca cgaaactgtt
2221tagtgggtaa gaactacaca atcaagatag ctgactttgg aatgagcagg aacctgtaca
2281gtggtgacta ttaccggatc cagggCCGGG CAGTGCTCCC TATCCGCTGG ATGTCTTGGG
2341AGAGTATCTT GCTGGGCAAG TTCACTACAG CAAGTGATGT GTGGGCCTTT GGGGTTACTT
2401TGTGGGAGAC TTTCACCTTT TGTCAAGAAC AGCCCTATTC CCAGCTGTCA GATGAACAGG
2461TTATTGAGAA TACTGGAGAG TTCTTCCGAG ACCAAGGGAG GCAGACTTAC CTCCCTCAAC
2521CAGCCATTTG TCCTGACTCT GTGTATAAGC TGATGCTCAG CTGCTGGAGA AGAGATACGA
2581AGAACCGTCC CTCATTCCAA GAAATCCACC TTCTGCTCCT TCAACAAGGC GACGAGTAGG
2641ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
2701GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
2761TTTATATATC TTGTGGAAAG GACGAAATAC TATCCATGAT CAAATACACA CGCGTTAAGT
2821Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)