Construct: ORF TRCN0000489118
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020456.1_s317c1
- DNA Barcode:
- CTCACACACCATTGACACCAGAAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CLK2 (1196)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489118
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1196 | CLK2 | CDC like kinase 2 | NM_001294338.2 | 98.6% | 37% | 551_552insCAGCCTCTCCTGCCAACTTA |
| 2 | human | 1196 | CLK2 | CDC like kinase 2 | NM_003993.4 | 98.4% | 36.8% | 398_399insGCA;548_549insCAGCCTCTCCTGCCAACTTA |
| 3 | human | 1196 | CLK2 | CDC like kinase 2 | XM_011509143.2 | 98.4% | 36.8% | 168_169insAGC;548_549insCAGCCTCTCCTGCCAACTTA |
| 4 | human | 1196 | CLK2 | CDC like kinase 2 | NM_001363704.2 | 98.2% | 36.6% | 168_169insAGC;395_396insGCA;545_546insCAGCCTCTCCTGCCAACTTA |
| 5 | human | 1197 | CLK2P1 | CDC like kinase 2, pseudoge... | NR_002711.3 | 61.2% | (many diffs) | |
| 6 | human | 1196 | CLK2 | CDC like kinase 2 | NM_001294339.2 | 53.5% | 10.2% | 0_1ins704 |
| 7 | mouse | 12748 | Clk2 | CDC-like kinase 2 | NM_007712.4 | 90.9% | 35.3% | (many diffs) |
| 8 | mouse | 12748 | Clk2 | CDC-like kinase 2 | NM_001163432.2 | 90.8% | 35.1% | (many diffs) |
| 9 | mouse | 105943584 | Gm45927 | predicted gene, 45927 | NM_001310016.1 | 56.5% | 23.2% | (many diffs) |
| 10 | mouse | 105943584 | Gm45927 | predicted gene, 45927 | NM_001310017.1 | 30.3% | 8.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 666
- ORF length:
- 594
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgccgcat cctcgaaggt accactcctc agagcgaggc agccggggga 121 gttaccgtga acactatcgg agccgaaagc ataagcgacg aagaagtcgc tcctggtcaa 181 gtagtagtga ccggacacga cggcgtcggc gagaggacag ctaccatgtc cgttctcgaa 241 gcagttatga tgatcgttcg tccgaccgga gggtgtatga ccggcgatac tgtggcagct 301 acagacgcaa cgattatagc cgggatcggg gagatgccta ctatgacaca gactatcggc 361 attcctatga atatcagcgg gagaacagca gttaccgcag ccagcgcagc agccggagga 421 agcacagacg gcggaggagg cgcagccgga catttagccg ctcatcttcg cagcacagca 481 gccggagagc caagagtgta gaggacgacg ctgagggcca cctcatctac cacgtcgggg 541 actggctaca agagcgatat gaaatcgtta gcaccttagg agaggggacc ttcggccgag 601 ttgtacaatg tgttgaccat cgcagcctct cctgccaact tacaggggtg gggctcgagt 661 tgccctgaag atcattaaga atgtggagaa gtacaaggaa gcagctcgac ttgagatcaa 721 cgtgctagag aaaatcaatg agaaagaccc tgacaacaag aacctctgtg tccagatgtt 781 tgactggttt gactaccatg gccacatgtg tatctccttt gagcttctgg gccttagcac 841 cttcgatttc ctcaaagaca acaactacct gccctacccc atccaccaag tgcgccacat 901 ggccttccag ctgtgccagg ctgtcaagtt cctccatgat aacaagctga cacatacaga 961 cctcaagcct gaaaatattc tgtttgtgaa ttcagactat gagctcacct acaacctaga 1021 gaagaagcga gatgagcgca gtgtgaagag cacagctgtg cgggtggtag actttggcag 1081 tgccaccttt gaccatgagc accatagcac cattgtctcc actcgccatt accgagcacc 1141 agaagtcatc cttgagttgg gctggtcaca gccttgtgat gtgtggagta taggctgcat 1201 catctttgaa tactatgtgg GATTCACCCT CTTCCAGACC CATGACAACA GAGAGCATCT 1261 AGCCATGATG GAAAGGATCT TGGGTCCTAT CCCTTCCCGG ATGATCCGAA AGACAAGAAA 1321 GCAGAAATAT TTTTACCGGG GTCGCCTGGA TTGGGATGAG AACACATCAG CTGGGCGCTA 1381 TGTTCGTGAG AACTGCAAAC CGCTGCGGCG GTATCTGACC TCAGAGGCAG AGGAACACCA 1441 CCAGCTCTTC GATCTGATTG AAAGCATGCT AGAGTATGAA CCAGCTAAGC GGCTGACCTT 1501 GGGTGAAGCC CTTCAGCATC CTTTCTTCGC CCGCCTTCGG GCTGAGCCGC CCAACAAGTT 1561 GTGGGACTCC AGTCGGGATA TCAGTCGGTA GGACCCAGCT TTCTTGTACA AAGTGGTTGA 1621 TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG 1681 TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACTC 1741 ACACACCATT GACACCAGAA TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt 1801 tgtgaaagat t