Construct: ORF TRCN0000489128
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019180.1_s317c1
- DNA Barcode:
- CAATTAAATGCAATAACCTTCTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LGR5 (8549)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489128
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8549 | LGR5 | leucine rich repeat contain... | NM_003667.4 | 99.9% | 100% | 882G>A |
| 2 | human | 8549 | LGR5 | leucine rich repeat contain... | NM_001277226.2 | 97.3% | 97.3% | 784_785ins72;810G>A |
| 3 | human | 8549 | LGR5 | leucine rich repeat contain... | NM_001277227.2 | 92% | 92% | 426_427ins216;666G>A |
| 4 | human | 8549 | LGR5 | leucine rich repeat contain... | NR_110596.2 | 43.9% | (many diffs) | |
| 5 | mouse | 14160 | Lgr5 | leucine rich repeat contain... | NM_010195.2 | 84.6% | 85.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 2796
- ORF length:
- 2721
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggac acctcccggc tcggtgtgct cctgtccttg cctgtgctgc 121 tgcagctggc gaccgggggc agctctccca ggtctggtgt gttgctgagg ggctgcccca 181 cacactgtca ttgcgagccc gacggcagga tgttgctcag ggtggactgc tccgacctgg 241 ggctctcgga gctgccttcc aacctcagcg tcttcacctc ctacctagac ctcagtatga 301 acaacatcag tcagctgctc ccgaatcccc tgcccagtct ccgcttcctg gaggagttac 361 gtcttgcggg aaacgctctg acatacattc ccaagggagc attcactggc ctttacagtc 421 ttaaagttct tatgctgcag aataatcagc taagacacgt acccacagaa gctctgcaga 481 atttgcgaag ccttcaatcc ctgcgtctgg atgctaacca catcagctat gtgcccccaa 541 gctgtttcag tggcctgcat tccctgaggc acctgtggct ggatgacaat gcgttaacag 601 aaatccccgt ccaggctttt agaagtttat cggcattgca agccatgacc ttggccctga 661 acaaaataca ccacatacca gactatgcct ttggaaacct ctccagcttg gtagttctac 721 atctccataa caatagaatc cactccctgg gaaagaaatg ctttgatggg ctccacagcc 781 tagagacttt agatttaaat tacaataacc ttgatgaatt ccccactgca attaggacac 841 tctccaacct taaagaacta ggatttcata gcaacaatat caggtcgata cctgagaaag 901 catttgtagg caacccttct cttattacaa tacatttcta tgacaatccc atccaatttg 961 ttgggagatc tgcttttcaa catttacctg aactaagaac actgactctg aatggtgcct 1021 cacaaataac tgaatttcct gatttaactg gaactgcaaa cctggagagt ctgactttaa 1081 ctggagcaca gatctcatct cttcctcaaa ccgtctgcaa tcagttacct aatctccaag 1141 tgctagatct gtcttacaac ctattagaag atttacccag tttttcagtc tgccaaaagc 1201 ttcagaaaat tgacctaaga cataatgaaa tctacgaaat taaagttgac actttccagc 1261 agttgcttag cctccgatcg ctgaatttgg cttggaacaa aattgctatt attcacccca 1321 atgcattttc cactttgcca tccctaataa agctggacct atcgtccaac ctcctgtcgt 1381 cttttcctat aactgggtta catggtttaa ctcacttaaa attaacagga aatcatgcct 1441 tacagagctt gatatcatct gaaaactttc cagaactcaa ggttatagaa atgccttatg 1501 cttaccagtg ctgtgcattt ggagtgtgtg agaatgccta taagatttct aatcaatgga 1561 ataaaggtga caacagcagt atggacgacc ttcataagaa agatgctgga atgtttcagg 1621 ctcaagatga acgtgacctt gaagatttcc tgcttgactt tgaggaagac ctgaaagccc 1681 ttcattcagt gcagtgttca ccttccccag gccccttcaa accctgtgaa cacctgcttg 1741 atggctggct gatcagaatt ggagtgtgga ccatagcagt tctggcactt acttgtaatg 1801 ctttggtgac ttcaacagtt ttcagatccc ctctgtacat ttcccccatt aaactgttaa 1861 ttggggtcat cgcagcagtg aacatgctca cgggagtctc cagtgccgtg ctggctggtg 1921 tggatgcgtt cacttttggc agctttgcac gacatggtgc ctggtgggag aatggggttg 1981 gttgccatgt cattggtttt ttgtccattt ttgcttcaga atcatctgtt ttcctgctta 2041 ctctggcagc cctggagcgt gggttctctg tgaaatattc tgcaaaattt gaaacgaaag 2101 ctccattttc tagcctgaaa gtaatcattt tgctctgtgc cctgctggcc ttgaccatgg 2161 ccgcagttcc cctgctgggt ggcagcaagt atggcgcctc ccctctctgc ctgcctttgc 2221 cttttgggga gcccagcacc atgggctaca tggtcgctct catcttgctc aattcccttt 2281 gcttcctcat gatgaccatt gcctacacca agctctactg caatttggac aagggagacc 2341 tggagaatat ttgggactgc tctatggtaa aacacattgc cctgttgctc ttcaccaact 2401 gcatcctaaa ctgccctgtg gctttcttgt ccttctcctc tttaataaac cTTACATTTA 2461 TCAGTCCTGA AGTAATTAAG TTTATCCTTC TGGTGGTAGT CCCACTTCCT GCATGTCTCA 2521 ATCCCCTTCT CTACATCTTG TTCAATCCTC ACTTTAAGGA GGATCTGGTG AGCCTGAGAA 2581 AGCAAACCTA CGTCTGGACA AGATCAAAAC ACCCAAGCTT GATGTCAATT AACTCTGATG 2641 ATGTCGAAAA ACAGTCCTGT GACTCAACTC AAGCCTTGGT AACCTTTACC AGCTCCAGCA 2701 TCACTTATGA CCTGCCTCCC AGTTCCGTGC CATCACCAGC TTATCCAGTG ACTGAGAGCT 2761 GCCATCTTTC CTCTGTGGCA TTTGTCCCAT GTCTCGACCC AGCTTTCTTG TACAAAGTGG 2821 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2881 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2941 ACAATTAAAT GCAATAACCT TCTTGACGCG TTAAGTCgac aatcaacctc tggattacaa 3001 aatttgtgaa agatt