Construct: ORF TRCN0000489138
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021723.1_s317c1
- DNA Barcode:
- ACCCCACAATCCATATCACTCTGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- EPHA7 (2045)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489138
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2045 | EPHA7 | EPH receptor A7 | XM_017010365.2 | 40.5% | 1.1% | 1_1762del;2049G>A;2722C>T |
| 2 | human | 2045 | EPHA7 | EPH receptor A7 | NM_001288629.2 | 40.3% | 1.1% | (many diffs) |
| 3 | human | 2045 | EPHA7 | EPH receptor A7 | XM_005248669.3 | 40.3% | 1.1% | 1_1777del;2064G>A;2737C>T |
| 4 | human | 2045 | EPHA7 | EPH receptor A7 | NM_004440.4 | 40.1% | 1.1% | (many diffs) |
| 5 | human | 2045 | EPHA7 | EPH receptor A7 | XR_001743218.2 | 15.1% | (many diffs) | |
| 6 | mouse | 13841 | Epha7 | Eph receptor A7 | XM_006537606.3 | 36.7% | 1.2% | (many diffs) |
| 7 | mouse | 13841 | Epha7 | Eph receptor A7 | NM_001290434.1 | 36.7% | 1.2% | (many diffs) |
| 8 | mouse | 13841 | Epha7 | Eph receptor A7 | NM_010141.4 | 36.6% | 1.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 79
- ORF end:
- 172
- ORF length:
- 93
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccca agaaggcgat gaagagcttt actttcattg caccaaaacc tacattgacc 121 ctgaaaccta tgaggaccca aatagagctg tccatcaatt cgccaaggag ctagatgcct 181 cctgtattaa aattgagcgt gtgattggtg caggagaatt cggtgaagtc tgcagtggcc 241 gtttgaaact tccagggaaa agagatgttg cagtagccat aaaaaccctg aaagttggtt 301 acacagaaaa acaaaggaga gactttttgt gtgaagcaag catcatgggg cagtttgacc 361 acccaaatgt tgtccatttg gaaggggttg ttacaagagg gaaaccagtc atgatagtaa 421 tagagttcat ggaaaatgga gccctagatg catttctcag gaaacatgat gggcaattta 481 cagtcattca gttagtagga atgctgagag gaattgctgc tggaatgaga tatttggctg 541 atatgggata tgttcacagg gaccttgcag ctcgcaatat tcttgtcaac agcaatctcg 601 tttgtaaagt gtcagatttt ggcctgtccc gagttataga ggatgatcca gaagctgtct 661 atacaactac tggtggaaaa attccagtaa ggtggacagc acccgaagcc atccagtacc 721 ggaaattcac atcagccagt gatgtatgga gctatggaat agtcatgtgg gaagttatgt 781 cttatggaga aagaccttat tgggacatgt caaatcaaga tgttataaaa gcaatagaag 841 aaggttatcg tttaccagca cccatggact gcccagctgg ccttcaccag ctaatgttgg 901 attgttggca aaaggagcgt gctgaaaggc caaaatttga acagatagtt ggaattctag 961 acaAAATGAT TCGAAACCCA AATAGTCTGA AAACTCCCCT GGGAACTTGT AGTAGGCCAA 1021 TAAGCCCTCT TCTGGATTAA AACACTCCTG ATTTCACTAC CTTTTGTTCA GTTGGAGAAT 1081 GGCTACAAGC TATTAAGATG GAAAGATATA AAGATAATTT CACGGCAGCT GGCTACAATT 1141 CCCTTGAATC AGTAGCCAGG ATGACTATTG AGGATGTGAT GAGTTTAGGG ATCACACTGG 1201 TTGGTCATCA AAAGAAAATC ATGAGCAGCA TTCAGACTAT GAGAGCACAA ATGCTACATT 1261 TACATGGAAC TGGCATTCAA GTGTGAGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1321 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1381 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACCCCACA 1441 ATCCATATCA CTCTGTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1501 aagatt