Construct: ORF TRCN0000489140
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021675.1_s317c1
- DNA Barcode:
- CTTCGCGGCGATATCCGATCTAAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TNFSF14 (8740)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489140
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8740 | TNFSF14 | TNF superfamily member 14 | NM_172014.3 | 99.8% | 99.5% | 532A>G |
| 2 | human | 8740 | TNFSF14 | TNF superfamily member 14 | NM_003807.4 | 84.8% | 84.5% | 112_219del;640A>G |
| 3 | human | 8740 | TNFSF14 | TNF superfamily member 14 | XM_017027417.1 | 84.8% | 84.5% | 112_219del;640A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 681
- ORF length:
- 612
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggaggagagt gtcgtacggc cctcagtgtt tgtggtggat ggacagaccg 121 acatcccatt cacgaggctg ggacgaagcc accggagaca gtcgtgcagt gtggcccggg 181 acggacctgc aggctcctgg gagcagctga tacaagagcg aaggtctcac gaggtcaacc 241 cagcagcgca tctcacaggg gccaactcca gcttgaccgg cagcgggggg ccgctgttat 301 gggagactca gctgggcctg gccttcctga ggggccTCAG CTACCACGAT GGGGCCCTTG 361 TGGTCACCAA AGCTGGCTAC TACTACATCT ACTCCAAGGT GCAGCTGGGC GGTGTGGGCT 421 GCCCGCTGGG CCTGGCCAGC ACCATCACCC ACGGCCTCTA CAAGCGCACA CCCCGCTACC 481 CCGAGGAGCT GGAGCTGTTG GTCAGCCAGC AGTCACCCTG CGGACGGGCC ACCAGCAGCT 541 CCCGGGTCTG GTGGGACAGC AGCTTCCTGG GTGGTGTGGT ACACCTGGAG GCTGGGGAGG 601 AGGTGGTCGT CCGTGTGCTG GATGAACGCC TGGTTCGACT GCGTGATGGT ACCCGGTCTT 661 ACTTCGGGGC TTTCATGGTG TAGAACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 721 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 781 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC TTCGCGGCGA 841 TATCCGATCT AAGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 901 att