Construct: ORF TRCN0000489145
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021153.1_s317c1
- DNA Barcode:
- CCAGCATCAAGAGTGATCTTGGCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TAS2R45 (259291)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489145
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 259291 | TAS2R45 | taste 2 receptor member 45 | NM_176886.2 | 99.4% | 98.3% | (many diffs) |
| 2 | human | 259289 | TAS2R43 | taste 2 receptor member 43 | XM_003960991.5 | 99.4% | 98.3% | (many diffs) |
| 3 | human | 259289 | TAS2R43 | taste 2 receptor member 43 | NM_176884.2 | 89.7% | 83.8% | (many diffs) |
| 4 | human | 259290 | TAS2R31 | taste 2 receptor member 31 | NM_176885.2 | 89.4% | 81.8% | (many diffs) |
| 5 | human | 259292 | TAS2R46 | taste 2 receptor member 46 | NM_176887.2 | 89.2% | 82.8% | (many diffs) |
| 6 | human | 259293 | TAS2R30 | taste 2 receptor member 30 | NM_001097643.1 | 85.6% | 76.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 969
- ORF length:
- 897
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgataact tttctgccca tcatattttc cattctagta gtggttacat 121 ttgttattgg aaattttgct aatggcttca tagcgttggt aaattccacc gagtgggtga 181 agagacaaaa gatctccttt gctgaccaaa ttgtcactgc tctggcggtc tccagagttg 241 gtttgctctg ggtgttatta ttaaattggt attcaactgt gttgaatcca gctttttgta 301 gtgtagaatt aagaactact gcttataata tctgggcagt aaccggccat ttcagcaact 361 ggcctgctac tagcctcagc atattttatt tgctcaagat tgccaatttc tccaacctta 421 tttttcttcg cttaaagagg agagttaaga gtgtcattct ggtgatgctg ttggggcctt 481 tgctattttt ggcttgtcat ctttttgtgg taaacatgaa tcagattgta tggacaaaag 541 aatatgaagg aaacatgact tggaagatca aattgaggcg tgcaatgtaC CTTTCAGATA 601 CGACTGTAAC CATGCTAGCA AACTTAGTAC CCTTTACTGT AACCCTGATA TCTTTTCTGC 661 TGTTAGTCTG TTCTCTGTGT AAACATCTCA AGAAGATGCA CCTCCATGGC AAAGGATCTC 721 AAGATCCCAG TACCAAGGTC CACATAAAAG TTTTGCAAAC TGTGATCTCC TTCCTCTTGT 781 TATGTGCCAT TTACTTTGTG TCTGTAATAA TATCAGTTTG GAGTTTTAAG AATCTGGAAA 841 ACAAACCTGT CTTCATGTTC TGCCAAGCTA TTGGATTCAG CTGTTCTTCA GCCCACCCGT 901 TCATCCTGAT TTGGGGAAAC AAGAAGCTAA AGCAGACTTA TCTTTCAGTT TTGTGGCAAA 961 TGAGGTACTG AGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1021 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1081 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCA GCATCAAGAG TGATCTTGGC 1141 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t