Construct: ORF TRCN0000489150

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020863.1_s317c1
DNA Barcode:
CGCTATTTTGTTTTATTATAACAC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
LPAR1 (1902)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489150
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1902 LPAR1 lysophosphatidic acid recep... NM_001351397.2 99.9% 100% 204A>G
2 human 1902 LPAR1 lysophosphatidic acid recep... NM_001351398.2 99.9% 100% 204A>G
3 human 1902 LPAR1 lysophosphatidic acid recep... NM_001351399.2 99.9% 100% 204A>G
4 human 1902 LPAR1 lysophosphatidic acid recep... NM_001351400.2 99.9% 100% 204A>G
5 human 1902 LPAR1 lysophosphatidic acid recep... NM_001351401.2 99.9% 100% 204A>G
6 human 1902 LPAR1 lysophosphatidic acid recep... NM_001351402.2 99.9% 100% 204A>G
7 human 1902 LPAR1 lysophosphatidic acid recep... NM_001351403.2 99.9% 100% 204A>G
8 human 1902 LPAR1 lysophosphatidic acid recep... NM_001351404.2 99.9% 100% 204A>G
9 human 1902 LPAR1 lysophosphatidic acid recep... NM_001351405.2 99.9% 100% 204A>G
10 human 1902 LPAR1 lysophosphatidic acid recep... NM_001351406.2 99.9% 100% 204A>G
11 human 1902 LPAR1 lysophosphatidic acid recep... NM_001351407.2 99.9% 100% 204A>G
12 human 1902 LPAR1 lysophosphatidic acid recep... NM_001351408.2 99.9% 100% 204A>G
13 human 1902 LPAR1 lysophosphatidic acid recep... NM_001351409.2 99.9% 100% 204A>G
14 human 1902 LPAR1 lysophosphatidic acid recep... NM_001351410.2 99.9% 100% 204A>G
15 human 1902 LPAR1 lysophosphatidic acid recep... NM_001351411.2 99.9% 100% 204A>G
16 human 1902 LPAR1 lysophosphatidic acid recep... NM_001351412.2 99.9% 100% 204A>G
17 human 1902 LPAR1 lysophosphatidic acid recep... NM_001351413.2 99.9% 100% 204A>G
18 human 1902 LPAR1 lysophosphatidic acid recep... NM_001351414.2 99.9% 100% 204A>G
19 human 1902 LPAR1 lysophosphatidic acid recep... NM_001351415.2 99.9% 100% 204A>G
20 human 1902 LPAR1 lysophosphatidic acid recep... NM_001351416.2 99.9% 100% 204A>G
21 human 1902 LPAR1 lysophosphatidic acid recep... NM_001351417.2 99.9% 100% 204A>G
22 human 1902 LPAR1 lysophosphatidic acid recep... NM_001351418.2 99.9% 100% 204A>G
23 human 1902 LPAR1 lysophosphatidic acid recep... NM_001351419.1 99.9% 100% 204A>G
24 human 1902 LPAR1 lysophosphatidic acid recep... NM_001351420.1 99.9% 100% 204A>G
25 human 1902 LPAR1 lysophosphatidic acid recep... NM_001401.5 99.9% 100% 204A>G
26 human 1902 LPAR1 lysophosphatidic acid recep... NM_057159.4 99.9% 100% 204A>G
27 human 1902 LPAR1 lysophosphatidic acid recep... XM_005251782.3 99.9% 100% 204A>G
28 human 1902 LPAR1 lysophosphatidic acid recep... XM_017014404.2 99.9% 100% 204A>G
29 mouse 14745 Lpar1 lysophosphatidic acid recep... NM_010336.2 90.4% 97.2% (many diffs)
30 mouse 14745 Lpar1 lysophosphatidic acid recep... NM_172989.1 90.4% 97.2% (many diffs)
31 mouse 14745 Lpar1 lysophosphatidic acid recep... XM_011249928.2 90.4% 97.2% (many diffs)
32 mouse 14745 Lpar1 lysophosphatidic acid recep... XM_011249929.2 90.4% 97.2% (many diffs)
33 mouse 14745 Lpar1 lysophosphatidic acid recep... XM_011249930.2 90.4% 97.2% (many diffs)
34 mouse 14745 Lpar1 lysophosphatidic acid recep... XM_011249931.2 90.4% 97.2% (many diffs)
35 mouse 14745 Lpar1 lysophosphatidic acid recep... XM_011249932.2 90.4% 97.2% (many diffs)
36 mouse 14745 Lpar1 lysophosphatidic acid recep... XM_011249933.2 90.4% 97.2% (many diffs)
37 mouse 14745 Lpar1 lysophosphatidic acid recep... XM_011249934.2 90.4% 97.2% (many diffs)
38 mouse 14745 Lpar1 lysophosphatidic acid recep... XM_011249935.2 90.4% 97.2% (many diffs)
39 mouse 14745 Lpar1 lysophosphatidic acid recep... NM_001290486.1 85.9% 92.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1164
ORF length:
1092
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggctgcc atctctactt ccatccctgt aatttcacag ccccagttca
121cagccatgaa tgaaccacag tgcttctaca acgagtccat tgccttcttt tataaccgaa
181gtggaaagca tcttgccaca gaatggaaca cagtcagcaa gctggtgatg ggacttggaa
241tcactgtttg tatcttcatc atgttggcca acctgttggt catggtggca atctatgtca
301accgccgctt ccattttcct atttattacc taatggctaa tctggctgct gcagacttct
361ttgctgggtt ggcctacttc tatctcatgt tcaacacagg acccaatact cggagactga
421ctgttagcac atggctcctt cgtcagggcc tcattgacac cagcctgacg gcatctgtgg
481ccaacttact ggctattgca atcgagaggc acattacggt tttccgcatg cagctccaca
541cacggatgag caaccggcgg gtagtggtgg tcattgtggt catctggact atggccatcg
601ttatgggtgc tatacccagt gtgggctgga actgtatctg tgatattgaa aattgttcca
661acatggcacc cctctacagt gactcttact tagtcttctg ggccattttc aacttggtga
721cctttgtggt aatggtggtt ctctatgctc acatctttGG CTATGTTCGC CAGAGGACTA
781TGAGAATGTC TCGGCATAGT TCTGGACCCC GGCGGAATCG GGATACCATG ATGAGTCTTC
841TGAAGACTGT GGTCATTGTG CTTGGGGCCT TTATCATCTG CTGGACTCCT GGATTGGTTT
901TGTTACTTCT AGACGTGTGC TGTCCACAGT GCGACGTGCT GGCCTATGAG AAATTCTTCC
961TTCTCCTTGC TGAATTCAAC TCTGCCATGA ACCCCATCAT TTACTCCTAC CGCGACAAAG
1021AAATGAGCGC CACCTTTAGG CAGATCCTCT GCTGCCAGCG CAGTGAGAAC CCCACCGGCC
1081CCACAGAAGG CTCAGACCGC TCGGCTTCCT CCCTCAACCA CACCATCTTG GCTGGAGTTC
1141ACAGCAATGA CCACTCTGTG GTTTAGGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG
1201GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
1261AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACGCTATTT
1321TGTTTTATTA TAACACACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
1381aagatt

Download FASTA (ORF) (Full)