Construct: ORF TRCN0000489166
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019147.1_s317c1
- DNA Barcode:
- CAGTTATTTTCTAATCTTTCAAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ESYT1 (23344)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489166
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23344 | ESYT1 | extended synaptotagmin 1 | NM_015292.2 | 100% | 100% | |
| 2 | human | 23344 | ESYT1 | extended synaptotagmin 1 | NM_001184796.1 | 99.1% | 99.1% | 1474_1503del |
| 3 | mouse | 23943 | Esyt1 | extended synaptotagmin-like... | NM_011843.2 | 84.6% | 87.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 3387
- ORF length:
- 3312
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggag cgatctccag gagagggccc cagccccagc cccatggacc 121 agccctctgc tccctccgac cccactgacc agccccccgc tgctcacgca aagccagacc 181 caggttctgg gggccaacct gctggccctg gcgcggcggg tgaggccctg gcggtgctga 241 cttcattcgg gaggcggttg ctggtgctga tacctgtgta tttggccggg gcagtgggac 301 tcagcgtggg tttcgtgctc ttcggcctcg ccctctacct gggctggcgc cgggtccgcg 361 acgagaaaga acggagcctt cgagcagcga ggcagctact ggacgacgag gagcagctca 421 ctgcgaaaac tctctatatg agtcatcgag agctacctgc ctgggtcagc ttcccagacg 481 tggaaaaggc tgaatggctc aataagattg tggcccaggt ctggcccttc ctgggccagt 541 atatggagaa gcttctggct gaaactgtgg ctccggctgt taggggatct aacccccatc 601 tgcaaacatt tacatttaca cgagtggaac tgggtgaaaa gccattgcgc atcattggag 661 tcaaggttca cccaggtcag agaaaagagc agatcctgct ggacttgaac atcagctatg 721 taggtgatgt gcagattgat gtggaagtga agaaatattt ttgcaaagca ggagtcaagg 781 gcatgcagct acatggcgtt ttgcgggtga tactggagcc actcattggg gaccttccct 841 tcgtgggggc tgtgtcaatg ttcttcatcc gacgcccgac cctagacatc aactggacag 901 ggatgaccaa cctgctggat atcccaggac ttagctcact ctctgacacc atgatcatgg 961 actccattgc tgccttcctc gtgttgccca accgattact ggtgcccctt gtgcctgacc 1021 ttcaagatgt ggctcagttg cgttcccctc tgcccagggg cattattcga attcacctgc 1081 tggctgctcg agggctgagt tccaaggaca aatatgtgaa gggcctgatt gagggcaagt 1141 cagacccata tgcacttgtg cgtttgggta cccagacatt ctgcagtcgt gtcattgatg 1201 aagaactcaa cccacagtgg ggagagactt atgaggtgat ggtacacgag gtcccagggc 1261 aggagattga agtggaggtg ttcgacaagg atccagataa agatgacttt ctgggcagaa 1321 tgaagctgga tgtagggaag gtgttacagg ctagcgttct ggatgattgg ttccctctac 1381 aaggtgggca aggccaagtt cacttgaggc tagaatggct gtcacttttg tcagatgcag 1441 agaaactgga gcaggttcta cagtggaatt ggggagtctc ctctcgacca gatcccccgt 1501 cagctgccat cttagttgtc tacctggatc gggcccagga tcttcctctg aagaagggga 1561 acaaggaacc caaccctatg gtacaactgt caattcagga tgtgactcag gagagcaagg 1621 ctgtctacag taccaactgc ccagtgtggg aggaagcgtt ccggttcttc ctacaagacc 1681 ctcaaagcca ggagctcgat gtgcaagtga aggatgattc cagggccctg actttaggag 1741 cactgacgct gcctctggcc cgcctgctga ctgccccaga actcatcctg gaccagtggt 1801 tccagctcag cagctctggt ccaaactcca gactctatat gaaactagtc atgaggatcc 1861 tgtacttgga ttcatcagaa atatgcttcc ccacggtgcc tggttgtcct ggtgcttggg 1921 acgtggacag tgagaatccc cagagaggca gcagtgtgga tgccccacct cgaccctgtc 1981 acacgactcc tgatagccag tttgggactg agcatgtgct tcggatccat gtattagagg 2041 cccaggacct gattgccaaa gaccgtttct tggggggact ggtgaagggc aagtcagacc 2101 cctatgtcaa actaaagttg gcaggacgaa gcttccggag ccatgttgtt cgggaagatc 2161 tcaatccccg ctggaatgag gtttttgagg tgatcgtcac atcagttcca ggccaagagc 2221 tagaggttga agtctttgac aaggacttgg acaaggatga ttttctgggc aggtgtaaag 2281 tgcgtctcac cacagtctta aacagtggct tccttgatga gtggctgacc ctggaggatg 2341 tcccatctgg ccgcctgcac ttgcgcctgg agcgtctcac cccccgtccc actgctgctg 2401 agttagagga ggtgctgcag gtgaatagtt tgatccagac tcagaagagt gcggagctgg 2461 ctgcggccct gctatccatc tatatggagc gggcagagga cctcccgctg cgaaaaggca 2521 ccaagcacct cagcccttat gctactctca ctgtgggaga tagttctcat aaaaccaaga 2581 ctatttcgca aacttcagcc cctgtctggg atgagagtgc ctcctttctc atcaggaaac 2641 cacacactga gagcctagag ttgcaggttc ggggtgaggg cactggcgtg ctgggctcat 2701 tatccctgcc cctctcagag ctcctcgtgg ctgaccagct ctgcttggac cgctggttta 2761 cactcagcag tggtcagggg caggtgctac tgagagcaca gctagggatc ctggtgtccc 2821 agcactcggg agtggaagct catagccaca gctacagcca cagctcctca tcgctgagtg 2881 aagaaccaga gctctcgggg ggaccccctc acatcacctc ctcagcccca gagctccggc 2941 agcgcctaac acatgttgac agtccccttg aggctccagc cgggcctctg ggccaggtga 3001 aactgactct gtggtactac agtgaagaac gaaagctggt cagcattgtt catggttgcc 3061 ggtcccttcG ACAGAATGGA CGTGATCCTC CTGATCCCTA TGTGTCACTG TTGCTACTGC 3121 CAGACAAGAA CCGAGGCACC AAGAGGAGGA CCTCACAGAA GAAGAGGACC CTGAGTCCTG 3181 AATTTAATGA ACGGTTTGAG TGGGAACTCC CCCTGGATGA GGCCCAGAGA CGAAAGCTGG 3241 ATGTCTCTGT CAAGTCTAAT TCCTCCTTCA TGTCAAGAGA GCGTGAGCTG CTGGGGAAGG 3301 TGCAGCTGGA CCTAGCTGAG ACAGACCTTT CCCAGGGTGT AGCCCGGTGG TATGACCTGA 3361 TGGACAACAA GGACAAGGGC AGCTCCGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 3421 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 3481 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACAGTTATT 3541 TTCTAATCTT TCAAAAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 3601 aagatt