Construct: ORF TRCN0000489170
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019640.1_s317c1
- DNA Barcode:
- TTCATTGCATATTCACCCCTCGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DYRK2 (8445)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489170
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8445 | DYRK2 | dual specificity tyrosine p... | NM_006482.3 | 99.9% | 100% | 1491G>T |
| 2 | human | 8445 | DYRK2 | dual specificity tyrosine p... | NM_003583.4 | 87.7% | 87.8% | 0_1ins219;1272G>T |
| 3 | human | 8445 | DYRK2 | dual specificity tyrosine p... | XM_017020032.1 | 87.7% | 87.8% | 0_1ins219;1272G>T |
| 4 | mouse | 69181 | Dyrk2 | dual-specificity tyrosine-(... | NM_001014390.2 | 90.4% | 97.1% | (many diffs) |
| 5 | mouse | 69181 | Dyrk2 | dual-specificity tyrosine-(... | XM_006514046.3 | 80.4% | 86.2% | (many diffs) |
| 6 | mouse | 69181 | Dyrk2 | dual-specificity tyrosine-(... | XM_011243552.2 | 79% | 86% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 1893
- ORF length:
- 1803
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcaccgaa ttcgcggcca tgttaaccag gaaaccttcg gccgccgctc 121 ccgccgccta cccgaccggc cgaggtgggg acagcgccgt tcgtcagctt caggcttccc 181 cggggctcgg tgcaggggcc acccggagcg gagtggggac tggcccgccc tcccccatcg 241 ccctgccgcc tctccgggcc agcaacgctg ccgccgcagc ccacacgatt ggcggcagta 301 agcacacaat gaatgatcac ctgcatgtcg gcagccacgc tcacggacag atccaggttc 361 aacagttgtt tgaggataac agtaacaagc ggacagtgct cacgacacaa ccaaatgggc 421 ttacaacagt gggcaaaacg ggcttgccag tggtgccaga gcggcagctg gacagcattc 481 atagacggca ggggagctcc acctctctaa agtccatgga aggcatgggg aaggtgaaag 541 ccacccccat gacacctgaa caagcaatga agcaatacat gcaaaaactc acagccttcg 601 aacaccatga gattttcagc taccctgaaa tatatttctt gggtctaaat gctaagaagc 661 gccagggcat gacaggtggg cccaacaatg gtggctatga tgatgaccag ggatcatatg 721 tgcaggtgcc ccacgatcac gtggcttaca ggtatgaggt cctcaaggtc attgggaagg 781 ggagctttgg gcaggtggtc aaggcctacg atcacaaagt ccaccagcac gtggccctaa 841 agatggtgcg gaatgagaag cgcttccacc ggcaagcagc ggaggagatc cgaatcctgg 901 aacacctgcg gaagcaggac aaggataaca caatgaatgt catccatatg ctggagaatt 961 tcaccttccg caaccacatc tgcatgacgt ttgagctgct gagcatgaac ctctatgagc 1021 tcatcaagaa gaataaattc cagggcttca gtctgccttt ggttcgcaag tttgcccact 1081 cgattctgca gtgcttggat gctttgcaca aaaacagaat aattcactgt gaccttaagc 1141 ccgagaacat tttgttaaag cagcagggta gaagcggtat taaagtaatt gattttggct 1201 ccagttgtta cgagcatcag cgtgtctaca cgtacatcca gtcgcgtttt taccgggctc 1261 cagaagtgat ccttggggcc aggtatggca tgcccattga tatgtggagc ctgggctgca 1321 ttttagcaga gctcctgacg ggttaccccc tcttgcctgg ggaagatgaa ggggaccagc 1381 tggcctgtat gattgaactg ttgggcatgc cctcacagaa actgctggat gcatccaaac 1441 gagccaaaaa ttttgtgagc tccaagggtt atccccgtta ctgcactgtc acgactctct 1501 cagatggctc tgtggtccta aacggaggCC GTTCCCGGAG GGGGAAACTG AGGGGCCCAC 1561 CGGAGAGCAG AGAGTGGGGT AACGCGCTGA AGGGGTGTGA TGATCCCCTT TTCCTTGACT 1621 TCTTAAAACA GTGTTTAGAG TGGGATCCTG CAGTGCGCAT GACCCCAGGC CAGGCTTTGC 1681 GGCACCCCTG GCTGAGGAGG CGGTTGCCAA AGCCTCCCAC CGGGGAGAAA ACGTCAGTGA 1741 AAAGGATAAC TGAGAGCACC GGTGCTATCA CATCTATATC CAAGTTACCT CCACCTTCTA 1801 GCTCAGCTTC CAAACTGAGG ACTAATTTGG CGCAGATGAC AGATGCCAAT GGGAATATTC 1861 AGCAGAGGAC AGTGTTGCCA AAACTTGTTA GCAAGGGTGG GCGCGCCGAC CCAGCTTTCT 1921 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1981 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2041 GTGGAAAGGA CGATTCATTG CATATTCACC CCTCGTTACG CGTTAAGTCg acaatcaacc 2101 tctggattac aaaatttgtg aaagatt