Construct: ORF TRCN0000489196
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019244.1_s317c1
- DNA Barcode:
- GACACCGGACTCAGCGCACGGTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAMP3 (10268)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489196
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10268 | RAMP3 | receptor activity modifying... | NM_005856.3 | 99.7% | 99.3% | 444_445insG |
| 2 | human | 10268 | RAMP3 | receptor activity modifying... | XM_017011666.1 | 78.2% | 77.8% | 0_1ins96;348_349insG |
| 3 | human | 10268 | RAMP3 | receptor activity modifying... | XM_006715631.3 | 57% | 56.9% | 58_390del;777_778insG |
| 4 | mouse | 56089 | Ramp3 | receptor (calcitonin) activ... | XM_006514761.3 | 66.9% | 69.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 522
- ORF length:
- 447
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggag actggagcgc tgcggcgccc gcaacttctc ccgttgctgc 121 tgctgcTCTG CGGTGGGTGT CCCAGAGCAG GCGGCTGCAA CGAGACAGGC ATGTTGGAGA 181 GGCTGCCCCT GTGTGGGAAG GCTTTCGCAG ACATGATGGG CAAGGTGGAC GTCTGGAAGT 241 GGTGCAACCT GTCCGAGTTC ATCGTGTACT ATGAGAGTTT CACCAACTGC ACCGAGATGG 301 AGGCCAATGT CGTGGGCTGC TACTGGCCCA ACCCCCTGGC CCAGGGCTTC ATCACCGGCA 361 TCCACAGGCA GTTCTTCTCC AACTGCACCG TGGACAGGGT CCACTTGGAG GACCCCCCAG 421 ACGAGGTTCT CATCCCGCTG ATCGTTATAC CCGTCGTTCT GACTGTCGCC ATGGCTGGCC 481 TGGTGGTGTG GCGCAGCAAA CGCACCGACA CGCTGCTGGA CCCAGCTTTC TTGTACAAAG 541 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 601 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 661 ACGAGACACC GGACTCAGCG CACGGTTGAC GCGTTAAGTC gacaatcaac ctctggatta 721 caaaatttgt gaaagatt