Construct: ORF TRCN0000489201
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019643.1_s317c1
- DNA Barcode:
- CGAACGTACCCACTCGCACTACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GABBR2 (9568)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489201
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9568 | GABBR2 | gamma-aminobutyric acid typ... | NM_005458.8 | 93.7% | 93.7% | (many diffs) |
| 2 | human | 9568 | GABBR2 | gamma-aminobutyric acid typ... | XM_017015331.2 | 92.8% | 87.8% | (many diffs) |
| 3 | human | 9568 | GABBR2 | gamma-aminobutyric acid typ... | XM_005252316.5 | 77.2% | 77.2% | 0_1ins600;1758T>C;2049_2050insG |
| 4 | human | 9568 | GABBR2 | gamma-aminobutyric acid typ... | XM_017015332.2 | 77.2% | 77.2% | 0_1ins600;1758T>C;2049_2050insG |
| 5 | mouse | 242425 | Gabbr2 | gamma-aminobutyric acid (GA... | NM_001081141.1 | 83.9% | 92.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2724
- ORF length:
- 2652
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggcctc atgccgctca ccaaggaggt ggccaagggc agcatcgggc 121 gcggtgtgct ccccgccgtg gaactggcca tcgagcagat ccgcaacgag tcactcctgc 181 gcccctactt cctcgacctg cggctctatg acacggagtg cgacaacgca aaagggttga 241 aagccttcta cgatgcaata aaatacgggc cgaaccactt gatggtgttt ggaggcgtct 301 gtccatccgt cacatccatc attgcagagt ccctccaagg ctggaatctg gtgcagcttt 361 cttttgctgc aaccacgcct gttctagccg ataagaaaaa atacccttat ttctttcgga 421 ccgtcccatc agacaatgcg gtgaatccag ccattctgaa gttgctcaag cactaccagt 481 ggaagcgcgt gggcacgctg acgcaagacg ttcagaggtt ctctgaggtg cggaatgacc 541 tgactggagt tctgtatggc gaggacattg agatttcaga caccgagagc ttctccaacg 601 atccctgtac cagtgtcaaa aagctgaagg ggaatgatgt gcggatcatc cttggccagt 661 ttgaccagaa tatggcagca aaagtgttct gttgtgcata cgaggagaac atgtatggta 721 gtaaatatca gtggatcatt ccgggctggt acgagccttc ttggtgggag caggtgcaca 781 cggaagccaa ctcatcccgc tgcctccgga agaatctgct tgctgccatg gagggctaca 841 ttggcgtgga tttcgagccc ctgagctcca agcagatcaa gaccatctca ggaaagactc 901 cacagcagta tgagagagag tacaacaaca agcggtcagg cgtggggccc agcaagttcc 961 acgggtacgc ctacgatggc atctgggtca tcgccaagac actgcagagg gccatggaga 1021 cactgcatgc cagcagccgg caccagcgga tccaggactt caactacacg gaccacacgc 1081 tgggcaggat catcctcaat gccatgaacg agaccaactt cttcggggtc acgggtcaag 1141 ttgtattccg gaatggggag agaatgggga ccattaaatt tactcaattt caagacagca 1201 gggaggtgaa ggtgggagag tacaacgctg tggccgacac actggagatc atcaatgaca 1261 ccatcaggtt ccaaggatcc gaaccaccaa aagacaagac catcatcctg gagcagctgc 1321 ggaagatctc cctacctctc tacagcatcc tctctgccct caccatcctc gggatgatca 1381 tggccagtgc ttttctcttc ttcaacatca agaaccggaa tcagaagctc ataaagatgt 1441 cgagtccata catgaacaac cttatcatcc ttggagggat gctctcctat gcttccatat 1501 ttctctttgg ccttgatgga tcctttgtct ctgaaaagac ctttgaaaca ctttgcaccg 1561 tcaggacctg gattctcacc gtgggctaca cgaccgcttt tggggccatg tttgcaaaga 1621 cctggagagt ccacgccatc ttcaaaaatg tgaaaatgaa gaagaagatc atcaaggacc 1681 agaaactgct tgtgatcgtg gggggcatgc tgctgatcga cctgtgtatc ctgatctgct 1741 ggcaggctgt ggaccccctg cgaaggacag tggagaagta cagcatggag ccggacccag 1801 caggacggga tatctccatc cgccctctcc tggagcactg tgagaacacc catatgacca 1861 tctggcttgg catcgtctat gcctacaagg gacttctcat gttgttcggt tgtttcttag 1921 cttgggagac ccgcaacgtc agcatccccg cactcaacga cagcaagtac atcgggatga 1981 gtgtctacaa cgtggggatc atgtgcatca tcggggccgc tgtctccttc ctgacccggg 2041 accagcccaa tgtgcagttc tgcatcgtgg ctctggtcat catcttctgc agcaccatca 2101 ccctctgcct ggtattcgtg ccgaagctca tcaccctgag aacaaaccca gatgcagcaa 2161 cgcagaacag gcgattccag ttcactcaga atcagaagaa agaagattct aaaacgtcca 2221 cctcggtcac cagtgtgaac caagccagca catcccgcct ggagggccta cagtcagaaa 2281 accatcgcct gcgaatgaag atcacagagc tggataaaga cttggaagag gtcaccatgc 2341 agctgcagga cacaccagaa aagaCCACCT ACATTAAACA GAACCACTAC CAAGAGCTCA 2401 ATGACATCCT CAACCTGGGA AACTTCACCG AGAGCACAGA TGGAGGAAAG GCCATTTTAA 2461 AAAATCACCT CGATCAAAAT CCCCAGCTAC AGTGGAACAC AACAGAGCCC TCTCGAACAT 2521 GCAAAGATCC TATAGAAGAT ATAAACTCTC CAGAACACAT CCAGCGTCGG CTGTCCCTCC 2581 AGCTCCCCAT CCTCCACCAC GCCTACCTCC CATCCATCGG AGGCGTGGAC GCCAGCTGTG 2641 TCAGCCCCTG CGTCAGCCCC ACCGCCAGCC CCCGCCACAG ACATGTGCCA CCCTCCTTCC 2701 GAGTCATGGT CTCGGGCCTG GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2761 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2821 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACGAA CGTACCCACT 2881 CGCACTACCT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt