Construct: ORF TRCN0000489212
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021362.1_s317c1
- DNA Barcode:
- TGAGTGTTCGTGGAGTGATAAGGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SMOC1 (64093)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489212
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64093 | SMOC1 | SPARC related modular calci... | NM_001034852.3 | 100% | 100% | |
| 2 | human | 64093 | SMOC1 | SPARC related modular calci... | NM_022137.6 | 99.7% | 99.7% | 1289_1290insAGT |
| 3 | human | 64093 | SMOC1 | SPARC related modular calci... | XM_005267995.1 | 97.5% | 97.5% | 525_557del |
| 4 | human | 64093 | SMOC1 | SPARC related modular calci... | XM_005267996.1 | 97.3% | 97.3% | 525_557del;1322_1323insAGT |
| 5 | mouse | 64075 | Smoc1 | SPARC related modular calci... | NM_022316.2 | 86.3% | 92.2% | (many diffs) |
| 6 | mouse | 64075 | Smoc1 | SPARC related modular calci... | NM_001146217.1 | 84.2% | 90% | (many diffs) |
| 7 | mouse | 64075 | Smoc1 | SPARC related modular calci... | XM_006516135.3 | 79.4% | 81.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1377
- ORF length:
- 1305
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgctgccc gcgcgctgcg cccgcctgct cacgccccac ttgctgctgg 121 tgttggtgca gctgtcccct gctcgcggcc accgcaccac aggccccagg tttctaataa 181 gtgaccgtga cccacagtgc aacctccact gctccaggac tcaacccaaa cccatctgtg 241 cctctgatgg caggtcctac gagtccatgt gtgagtacca gcgagccaag tgccgagacc 301 cgaccctggg cgtggtgcat cgaggtagat gcaaagatgc tggccagagc aagtgtcgcc 361 tggagcgggc tcaagccctg gagcaagcca agaagcctca ggaagctgtg tttgtcccag 421 agtgtggcga ggatggctcc tttacccagg tgcagtgcca tacttacact gggtactgct 481 ggtgtgtcac cccggatggg aagcccatca gtggctcttc tgtgcagaat aaaactcctg 541 tatgttcagg ttcagtcacc gacaagccct tgagccaggg taactcagga aggaaagatg 601 acgggtctaa gccgacaccc acgatggaga cccagccggt gttcgatgga gatgaaatca 661 cagccccaac tctatggatt aaacacttgg tgatcaagga ctccaaactg aacaacacca 721 acataagaaa ttcagagaaa gtctattcgt gtgaccagga gaggcagagt gccctggaag 781 aggcccagca gaatccccgt gagggtattg tcatccctga atgtgcccct gggggactct 841 ataagccagt gcaatgccac cagtccactg gctactgctg gtgtgtgctg gtggacacag 901 ggcgcccgct gcctgggacc tccacacgcT ACGTGATGCC CAGTTGTGAG AGCGACGCCA 961 GGGCCAAGAC TACAGAGGCG GATGACCCCT TCAAGGACAG GGAGCTACCA GGCTGTCCAG 1021 AAGGGAAGAA AATGGAGTTT ATCACCAGCC TACTGGATGC TCTCACCACT GACATGGTTC 1081 AGGCCATTAA CTCAGCAGCG CCCACTGGAG GTGGGAGGTT CTCAGAGCCA GACCCCAGCC 1141 ACACCCTGGA GGAGCGGGTA GTGCACTGGT ATTTCAGCCA GCTGGACAGC AATAGCAGCA 1201 ACGACATTAA CAAGCGGGAG ATGAAGCCCT TCAAGCGCTA CGTGAAGAAG AAAGCCAAGC 1261 CCAAGAAATG TGCCCGGCGT TTCACCGACT ACTGTGACCT GAACAAAGAC AAGGTCATTT 1321 CACTGCCTGA GCTGAAGGGC TGCCTGGGTG TTAGCAAAGA AGTAGGACGC CTCGTCTAGA 1381 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1441 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1501 TTTATATATC TTGTGGAAAG GACGATGAGT GTTCGTGGAG TGATAAGGCA CGCGTTAAGT 1561 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt