Construct: ORF TRCN0000489230
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019712.1_s317c1
- DNA Barcode:
- GTATATTTGCGGGGCGTTATACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCYL2 (55681)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489230
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55681 | SCYL2 | SCY1 like pseudokinase 2 | NM_001317784.1 | 99.9% | 99.8% | 2787_2788insG |
| 2 | human | 55681 | SCYL2 | SCY1 like pseudokinase 2 | NM_017988.6 | 99.9% | 99.8% | 2787_2788insG |
| 3 | human | 55681 | SCYL2 | SCY1 like pseudokinase 2 | NM_001330253.1 | 99.5% | 99.4% | 1272_1283del;2799_2800insG |
| 4 | human | 55681 | SCYL2 | SCY1 like pseudokinase 2 | NM_001330254.1 | 99.5% | 99.4% | 1272_1283del;2799_2800insG |
| 5 | human | 55681 | SCYL2 | SCY1 like pseudokinase 2 | NM_001330256.1 | 81% | 80.9% | 0_1ins519;753_764del;2280_2281insG |
| 6 | mouse | 213326 | Scyl2 | SCY1-like 2 (S. cerevisiae) | NM_198021.2 | 88.7% | 92.9% | (many diffs) |
| 7 | mouse | 213326 | Scyl2 | SCY1-like 2 (S. cerevisiae) | NM_001310704.1 | 88.6% | 92.8% | (many diffs) |
| 8 | mouse | 213326 | Scyl2 | SCY1-like 2 (S. cerevisiae) | XM_011243394.2 | 88.3% | 92.5% | (many diffs) |
| 9 | mouse | 213326 | Scyl2 | SCY1-like 2 (S. cerevisiae) | XM_006513458.3 | 88.3% | 92.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 2865
- ORF length:
- 2790
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggag tccatgctta ataaattgaa gagtactgtt acaaaagtaa 121 cagctgatgt cactagtgct gtaatgggaa atcctgtcac tagagaattt gatgttggtc 181 gacacattgc cagtggtggc aatgggctag cttggaagat ttttaatggc acaaaaaagt 241 caacaaagca ggaagtggca gtttttgtct ttgataaaaa actgattgac aagtatcaaa 301 aatttgaaaa ggatcaaatc attgattctc taaaacgagg agtccaacag ttaactcggc 361 ttcgacaccc tcgacttctt actgtccagc atcctttaga agaatccagg gattgcttgg 421 cattttgtac agaaccagtt tttgccagtt tagccaatgt tcttggtaac tgggaaaatc 481 taccttcccc tatatctcca gacattaagg attataaact ttatgatgta gaaaccaaat 541 atggtttgct tcaggtttct gaaggattgt cattcttgca tagcagtgtg aaaatggtgc 601 atggaaatat cactcctgaa aatataattt tgaataaaag tggagcctgg aaaataatgg 661 gttttgattt ttgtgtatca tcaaccaatc cttctgaaca agagcctaaa tttccttgta 721 aagaatggga cccaaattta ccttcattgt gtcttccaaa tcctgaatat ttggctcctg 781 aatacatact ttctgtgagc tgtgaaacag ccagtgatat gtattcttta ggaactgtta 841 tgtatgctgt atttaataaa gggaaaccta tatttgaagt caacaagcaa gatatttaca 901 agagtttcag taggcagttg gatcagttga gtcgtttagg atctagttca cttacaaata 961 tacctgagga agttcgtgaa catgtaaagc tactgttaaa tgtaactccg actgtaagac 1021 cagatgcaga tcaaatgaca aagattccct tctttgatga tgttggtgca gtaacactgc 1081 aatattttga taccttattc caaagagata atcttcagaa atcacagttt ttcaaaggac 1141 tgccaaaggt tctaccaaaa ctgcccaagc gtgtcattgt gcagagaatt ttgccttgtt 1201 tgacttcaga atttgtaaac cctgacatgg taccttttgt tttgcccaat gttctactta 1261 ttgctgagga atgcaccaaa gaagaatatg tcaaattaat tcttcctgaa cttggccctg 1321 tgtttaagca gcaggagcca atccagattt tgttaatttt cctacaaaaa atggatttgc 1381 tactaaccaa aacccctcct gatgagataa agaacagtgt tctacccatg gtttacagag 1441 cactagaagc tccttccatt cagatccagg agctctgtct aaacatcatt ccaacctttg 1501 caaatcttat agactaccca tccatgaaaa acgctttgat accaagaatt aaaaatgctt 1561 gtctacaaac atcttccctt gcggttcgtg taaattcatt agtgtgctta ggaaagattt 1621 tggaatactt ggataagtgg tttgtacttg atgatatcct acccttctta caacaaattc 1681 catccaagga acctgcggtc ctcatgggaa ttttaggtat ttacaaatgt acttttactc 1741 ataagaagtt gggaatcacc aaagagcagc tggccggaaa agtgttgcct catcttattc 1801 ccctgagtat tgaaaacaat cttaatctta atcagttcaa ttctttcatt tccgtcataa 1861 aagaaatgct taatagattg gagtctgaac ataagactaa actggagcaa cttcatataa 1921 tgcaagaaca gcagaaatct ttggatatag gaaatcaaat gaatgtttct gaggagatga 1981 aagttacaaa tattgggaat cagcaaattg acaaagtttt taacaacatt ggagcagacc 2041 ttctgactgg cagtgagtcc gaaaataaag aggacgggtt acagaataaa cataaaagag 2101 catcacttac acttgaagaa aaacaaaaat tagcaaaaga acaagagcag gcacagaagc 2161 tgaaaagcca gcagcctctt aaaccccaag tgcacacacc tgttgctact gttaaacaga 2221 ctaaggactt gacagacaca ctgatggata atatgtcatc cttgaccagc ctttctgtta 2281 gtacccctaa atcttctgct tcaagtactt tcacttctgt tccttccatg ggcattggta 2341 tgatgttttc tacaccaact gataatacaa agagaaattt gacaaatggc ctaaatgcca 2401 atatgggctt tcagacttca ggattcaaca tgcccgttaa tacaaaccag aacttcTACA 2461 GTAGTCCAAG CACAGTTGGA GTGACCAAGA TGACTCTGGG AACACCTCCC ACTTTGCCAA 2521 ACTTCAATGC TTTGAGTGTT CCTCCTGCTG GTGCAAAGCA GACCCAACAA AGACCCACAG 2581 ATATGTCTGC CCTTAATAAT CTCTTTGGCC CTCAGAAACC CAAAGTTAGC ATGAACCAGT 2641 TATCACAACA GAAACCAAAT CAGTGGCTTA ATCAGTTTGT ACCTCCTCAA GGTTCTCCAA 2701 CTATGGGCAG TTCAGTAATG GGGACACAGA TGAACGTGAT AGGACAATCT GCTTTTGGTA 2761 TGCAGGGTAA TCCTTTCTTT AACCCACAGA ACTTTGCACA GCCACCAACT ACTATGACCA 2821 ATAGCAGTTC AGCTAGCAAT GATTTAAAAG ATCTTTTTGG GGACCCAGCT TTCTTGTACA 2881 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2941 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 3001 AGGACGAGTA TATTTGCGGG GCGTTATACA TACGCGTTAA GTCgacaatc aacctctgga 3061 ttacaaaatt tgtgaaagat t