Construct: ORF TRCN0000489235
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021771.1_s317c1
- DNA Barcode:
- GAGAGCCCGCTGGTGTCTGCATAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GCOM1 (145781)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489235
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100820829 | MYZAP | myocardial zonula adherens ... | NM_001018100.5 | 99.9% | 100% | 504A>G |
| 2 | human | 100820829 | MYZAP | myocardial zonula adherens ... | NM_152451.7 | 93.9% | 93.9% | 504A>G;1116_1117ins84 |
| 3 | human | 145781 | GCOM1 | GRINL1A complex locus 1 | NM_001018091.6 | 93.5% | 92.7% | (many diffs) |
| 4 | human | 145781 | GCOM1 | GRINL1A complex locus 1 | NM_001018090.6 | 82.7% | 79.8% | (many diffs) |
| 5 | human | 145781 | GCOM1 | GRINL1A complex locus 1 | NM_001285900.3 | 59.9% | 56.6% | (many diffs) |
| 6 | human | 145781 | GCOM1 | GRINL1A complex locus 1 | NR_104369.2 | 29.1% | (many diffs) | |
| 7 | human | 145781 | GCOM1 | GRINL1A complex locus 1 | NR_104370.2 | 28.9% | (many diffs) | |
| 8 | human | 145781 | GCOM1 | GRINL1A complex locus 1 | NR_104368.2 | 28.5% | (many diffs) | |
| 9 | human | 145781 | GCOM1 | GRINL1A complex locus 1 | NR_104367.2 | 24.8% | (many diffs) | |
| 10 | human | 145781 | GCOM1 | GRINL1A complex locus 1 | NR_104371.3 | 21.2% | (many diffs) | |
| 11 | mouse | 102371 | Myzap | myocardial zonula adherens ... | NM_001033208.4 | 86.2% | 89.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1467
- ORF length:
- 1398
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gctgcgctcc acgtccacgg tcaccctgct ctcgggcggc gccgccagga 121 cgcccggggc gcccagcagg agggcaaatg tttgcagact acggctgacc gtacctcctg 181 agagtccagt tcctgagcaa tgtgaaaaga agattgagag aaaagagcag cttcttgacc 241 tgagcaatgg agaacctacc aggaaacttc ctcagggtgt tgtttatggt gtggtgcgaa 301 gatcagatca aaatcagcag aaagaaatgg tggtgtatgg gtggtccacc agtcagctga 361 aagaagagat gaactacatc aaagatgtga gagccacttt ggaaaaggtg agaaagcgaa 421 tgtatggaga ctatgatgag atgagacaga agattcgaca gctcacccag gaactatcag 481 tttcccatgc tcagcaggag tatctggaga atcacatcca aacccagtcg tctgccctgg 541 atcgttttaa tgccatgaac tcagccttgg cgtcagattc cattggcctg cagaaaaccc 601 tcgtggatgt gactttggaa aacagcaaca ttaaggatca aatcagaaat ctgcagcaga 661 cgtatgaagc atccatggac aagctgaggg aaaagcagag gcagttggag gtagcgcaag 721 ttgaaaacca gctgctaaaa atgaaggtgg aatcgtccca agaagccaat gctgaggtga 781 tgcgagagat gaccaagaag ctgtacagcc agtatgagga gaagctgcag gaagaacaga 841 ggaagcacag tgctgagaag gaggctcttt tggaagaaac caatagtttt ctgaaagcga 901 ttgaagaagc caataaaaag atgcaagcag cagagatcag cctagaggag aaagaccaga 961 ggatcgggga gctggacagg ctgattgagc gcatggaaaa ggaacgtcat caactgcaac 1021 ttcaactcct agaacatgaa acagaaatgt ctggggagtt aactgattct gacaaggaaa 1081 ggtatcagca gttggaggag gcatcagcca gccTCCGTGA GCGGATCAGA CACCTAGATG 1141 ACATGGTGCA TTGCCAGCAG AAGAAAGTCA AGCAGATGGT CGAGGAGATT GAATCATTAA 1201 AGAAAAAGTT GCAACAGAAA CAGCTCTTAA TACTGCAGCT TTTAGAAAAG ATATCTTTCT 1261 TAGAAGGAGA GAATAATGAA CTACAAAGCA GGTTGGACTA TTTAACAGAA ACCCAGGCCA 1321 AGACCGAAGT GGAAACCAGA GAGATAGGAG TGGGCTGTGA TCTTCTACCC AGCCAAACAG 1381 GCAGGACTCG TGAAATTGTG ATGCCTTCTA GGAACTACAC CCCATACACA AGAGTCCTGG 1441 AGTTAACCAT GAAGAAAACT CTGACTTAGG ACCCAGCTTT CTTGTACAAA GTGGTTGATA 1501 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1561 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGAGAG 1621 CCCGCTGGTG TCTGCATAGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1681 tgaaagatt