Construct: ORF TRCN0000489243
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020599.1_s317c1
- DNA Barcode:
- GCAGTAGAATCCCAGTCAGCGGCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GRK2 (156)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489243
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 156 | GRK2 | G protein-coupled receptor ... | NM_001619.5 | 99.9% | 100% | 96C>A |
| 2 | human | 156 | GRK2 | G protein-coupled receptor ... | XM_024448376.1 | 95.9% | 93.8% | (many diffs) |
| 3 | human | 156 | GRK2 | G protein-coupled receptor ... | XM_011544773.1 | 95.2% | 94.9% | (many diffs) |
| 4 | human | 156 | GRK2 | G protein-coupled receptor ... | XR_001747779.1 | 57.8% | (many diffs) | |
| 5 | mouse | 110355 | Grk2 | G protein-coupled receptor ... | NM_001290818.1 | 90.2% | 98.8% | (many diffs) |
| 6 | mouse | 110355 | Grk2 | G protein-coupled receptor ... | NM_130863.2 | 84.3% | 92.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2139
- ORF length:
- 2067
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcggac ctggaggcgg tgctggccga cgtgagctac ctgatggcca 121 tggagaagag caaggccacg ccggccgcgc gcgccagcaa gaagatactg ctgcccgagc 181 ccagcatccg cagtgtcatg cagaagtacc tggaggaccg gggcgaggtg acctttgaga 241 agatcttttc ccagaagctg gggtacctgc tcttccgaga cttctgcctg aaccacctgg 301 aggaggccag gcccttggtg gaattctatg aggagatcaa gaagtacgag aagctggaga 361 cggaggagga gcgtgtggcc cgcagccggg agatcttcga ctcatacatc atgaaggagc 421 tgctggcctg ctcgcatccc ttctcgaaga gtgccactga gcatgtccaa ggccacctgg 481 ggaagaagca ggtgcctccg gatctcttcc agccatacat cgaagagatt tgtcaaaacc 541 tccgagggga cgtgttccag aaattcattg agagcgataa gttcacacgg ttttgccagt 601 ggaagaatgt ggagctcaac atccacctga ccatgaatga cttcagcgtg catcgcatca 661 ttgggcgcgg gggctttggc gaggtctatg ggtgccggaa ggctgacaca ggcaagatgt 721 acgccatgaa gtgcctggac aaaaagcgca tcaagatgaa gcagggggag accctggccc 781 tgaacgagcg catcatgctc tcgctcgtca gcactgggga ctgcccattc attgtctgca 841 tgtcatacgc gttccacacg ccagacaagc tcagcttcat cctggacctc atgaacggtg 901 gggacctgca ctaccacctc tcccagcacg gggtcttctc agaggctgac atgcgcttct 961 atgcggccga gatcatcctg ggcctggagc acatgcacaa ccgcttcgtg gtctaccggg 1021 acctgaagcc agccaacatc cttctggacg agcatggcca cgtgcggatc tcggacctgg 1081 gcctggcctg tgacttctcc aagaagaagc cccatgccag cgtgggcacc cacgggtaca 1141 tggctccgga ggtcctgcag aagggcgtgg cctacgacag cagtgccgac tggttctctc 1201 tggggtgcat gctcttcaag ttgctgcggg ggcacagccc cttccggcag cacaagacca 1261 aagacaagca tgagatcgac cgcatgacgc tgacgatggc cgtggagctg cccgactcct 1321 tctcccctga actacgctcc ctgctggagg ggttgctgca gagggatgtc aaccggagat 1381 tgggctgcct gggccgaggg gctcaggagg tgaaagagag cccctttttc cgctccctgg 1441 actggcagat ggtcttcttg cagaagtacc ctcccccgct gatcccccca cgaggggagg 1501 tgaacgcggc cgacgccttc gacattggct ccttcgatga ggaggacaca aaaggaatca 1561 agttactgga cagtgatcag gagctctacc gcaacttccc cctcaccatc tcggagcggt 1621 ggcagcagga ggtggcagag actgtcttcg acaccatcaa cgctgagaca gaccggctgg 1681 aggctcgcaa gaaagccaag aacaagcagc tgggccatga ggaagactac gccctgggca 1741 aggactgcat catgcatggc tacatgtcca agatgggcaa ccccttccTG ACCCAGTGGC 1801 AGCGGCGGTA CTTCTACCTG TTCCCCAACC GCCTCGAGTG GCGGGGCGAG GGCGAGGCCC 1861 CGCAGAGCCT GCTGACCATG GAGGAGATCC AGTCGGTGGA GGAGACGCAG ATCAAGGAGC 1921 GCAAGTGCCT GCTCCTCAAG ATCCGCGGTG GGAAACAGTT CATTTTGCAG TGCGATAGCG 1981 ACCCTGAGCT GGTGCAGTGG AAGAAGGAGC TGCGCGACGC CTACCGCGAG GCCCAGCAGC 2041 TGGTGCAGCG GGTGCCCAAG ATGAAGAACA AGCCGCGCTC GCCCGTGGTG GAGCTGAGCA 2101 AGGTGCCGCT GGTCCAGCGC GGCAGTGCCA ACGGCCTCTG AGACCCAGCT TTCTTGTACA 2161 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2221 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2281 AGGACGAGCA GTAGAATCCC AGTCAGCGGC AACGCGTTAA GTCgacaatc aacctctgga 2341 ttacaaaatt tgtgaaagat t