Construct: ORF TRCN0000489256
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019956.1_s317c1
- DNA Barcode:
- AAGCTAGACCGGCCGTAGCTCACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NR1D1 (9572)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489256
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9572 | NR1D1 | nuclear receptor subfamily ... | NM_021724.5 | 99.9% | 99.8% | 1842_1843insG |
| 2 | mouse | 217166 | Nr1d1 | nuclear receptor subfamily ... | NM_145434.4 | 89.9% | 95.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1917
- ORF length:
- 1845
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgacgacc ctggactcca acaacaacac aggtggcgtc atcacctaca 121 ttggctccag tggctcctcc ccaagccgca ccagccctga atccctctat agtgacaact 181 ccaatggcag cttccagtcc ctgacccaag gctgtcccac ctacttccca ccatccccca 241 ctggctccct cacccaagac ccggctcgct cctttgggag cattccaccc agcctgagtg 301 atgacggctc cccttcttcc tcatcttcct cgtcgtcatc ctcctcctcc ttctataatg 361 ggagcccccc tgggagtcta caagtggcca tggaggacag cagccgagtg tcccccagca 421 agagcaccag caacatcacc aagctgaatg gcatggtgtt actgtgtaaa gtgtgtgggg 481 acgttgcctc gggcttccac tacggtgtgc acgcctgcga gggctgcaag ggctttttcc 541 gtcggagcat ccagcagaac atccagtaca aaaggtgtct gaagaatgag aattgctcca 601 tcgtccgcat caatcgcaac cgctgccagc aatgtcgctt caagaagtgt ctctctgtgg 661 gcatgtctcg agacgctgtg cgttttgggc gcatccccaa acgagagaag cagcggatgc 721 ttgctgagat gcagagtgcc atgaacctgg ccaacaacca gttgagcagc cagtgcccgc 781 tggagacttc acccacccag caccccaccc caggccccat gggcccctcg ccaccccctg 841 ctccggtccc ctcacccctg gtgggcttct cccagtttcc acaacagctg acgcctccca 901 gatccccaag ccctgagccc acagtggagg atgtgatatc ccaggtggcc cgggcccatc 961 gagagatctt cacctacgcc catgacaagc tgggcagctc acctggcaac ttcaatgcca 1021 accatgcatc aggtagccct ccagccacca ccccacatcg ctgggaaaat cagggctgcc 1081 cacctgcccc caatgacaac aacaccttgg ctgcccagcg tcataacgag gccctaaatg 1141 gtctgcgcca ggctccctcc tcctaccctc ccacctggcc tcctggccct gcacaccaca 1201 gctgccacca gtccaacagc aacgggcacc gtctatgccc cacccacgtg tatgcagccc 1261 cagaaggcaa ggcacctgcc aacagtcccc ggcagggcaa ctcaaagaat gttctgctgg 1321 catgtcctat gaacatgtac ccgcatggac gcagtgggcg aacggtgcag gagatctggg 1381 aggatttctc catgagcttc acgcccgctg tgcgggaggt ggtagagttt gccaaacaca 1441 tcccgggctt ccgtgacctt tctcagcatg accaagtcac cctgcttaag gctggcacct 1501 ttgaggtgct gatggtgcgc tttgcttcgt tgttcaacgt gaaggaccag acagtgatgt 1561 tcctaagccg caccacctac agcctgcagg agcttggtgc catgggcatg ggagacctgc 1621 tcagtgccat gttcGACTTC AGCGAGAAGC TCAACTCCCT GGCGCTTACC GAGGAGGAGC 1681 TGGGCCTCTT CACCGCGGTG GTGCTTGTCT CTGCAGACCG CTCGGGCATG GAGAATTCCG 1741 CTTCGGTGGA GCAGCTCCAG GAGACGCTGC TGCGGGCTCT TCGGGCTCTG GTGCTGAAGA 1801 ACCGGCCCTT GGAGACTTCC CGCTTCACCA AGCTGCTGCT CAAGCTGCCG GACCTGCGGA 1861 CCCTGAACAA CATGCATTCC GAGAAGCTGC TGTCCTTCCG GGTGGACGCC CAGGACCCAG 1921 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1981 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2041 TATCTTGTGG AAAGGACGAA AGCTAGACCG GCCGTAGCTC ACAACGCGTT AAGTCgacaa 2101 tcaacctctg gattacaaaa tttgtgaaag att