Construct: ORF TRCN0000489267
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021772.1_s317c1
- DNA Barcode:
- TTCGTCAAGATTATATCTACCTTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- POLR2M (81488)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489267
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81488 | POLR2M | RNA polymerase II subunit M | NM_015532.5 | 99.9% | 100% | 183C>T |
| 2 | human | 81488 | POLR2M | RNA polymerase II subunit M | NM_001018102.2 | 57.3% | 57.3% | 113_114ins471 |
| 3 | human | 145781 | GCOM1 | GRINL1A complex locus 1 | NM_001285900.3 | 46.4% | 43.5% | (many diffs) |
| 4 | human | 145781 | GCOM1 | GRINL1A complex locus 1 | NR_104371.3 | 17.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1173
- ORF length:
- 1104
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gtgctcgctg ccccgcggct tcgagcccca agctcccgag gacttggcgc 121 agcggagttt ggtggagctg cgggaaatgt tgaagcgcca ggagagactt ttgcgcaacg 181 aaaaattcat ttgcaaattg cccgacaaag gtaaaaagat ctttgactct tttgccaaac 241 tgaaagctgc tattgcagaa tgtgaagaag ttagaagaaa aagtgaactg tttaaccctg 301 ttagtttaga ctgtaagcta aggcaaaaag caattgcaga agttgatgtg ggtacagata 361 aggcccagaa ttctgacccg atacttgata cttcatcact agttcctgga tgttcctctg 421 tagataacat caagtcatct caaacctcac aaaaccaggg acttggacgt cctactcttg 481 aaggtgatga agagacttca gaggttgagt acacagtgaa taagggccca gcttccagca 541 atagagacag ggtaccacct tcatctgaag ctagtgagca tcacccgcgg catcgtgttt 601 caagtcaagc ggaagatact tccagcagct ttgacaacct gtttattgac aggttacaga 661 ggatcaccat tgcggaccaa ggtgaacaac agtcagaaga aaacgcaagt actaagaact 721 tgacaggcct ttccagtggg actgagaaga aacctcatta catggaagtg ctagaaatgc 781 gagccaaaaa cccagtgccc cagctgcgta aatttaaaac caatgtgtta ccTTTTCGAC 841 AAAATGATTC ATCTAGTCAT TGCCAGAAGA GTGGGTCTCC TATTTCCTCA GAAGAGCGGC 901 GGCGCAGGGA TAAGCAGCAT CTTGATGACA TCACAGCAGC TCGGCTTCTA CCACTTCACC 961 ATATGCCCAC GCAGCTGCTC TCCATAGAAG AATCCTTGGC ACTTCAGAAA CAGCAGAAAC 1021 AGAATTATGA GGAGATGCAA GCAAAGCTCG CAGCGCAAAA ATTAGCTGAA AGACTGAATA 1081 TTAAAATGCG GAGTTATAAT CCAGAAGGGG AGTCTTCAGG GAGATACCGA GAAGTAAGGG 1141 ATGAAGATGA CGATTGGTCC TCTGATGAAT TCTGAGACCC AGCTTTCTTG TACAAAGTGG 1201 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1261 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1321 ATTCGTCAAG ATTATATCTA CCTTTACGCG TTAAGTCgac aatcaacctc tggattacaa 1381 aatttgtgaa agatt