Construct: ORF TRCN0000489269
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021674.1_s317c1
- DNA Barcode:
- GATCGCCCAGCTGTTAGGCCCAGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TNFRSF14 (8764)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489269
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8764 | TNFRSF14 | TNF receptor superfamily me... | NM_003820.3 | 100% | 100% | |
| 2 | human | 8764 | TNFRSF14 | TNF receptor superfamily me... | XM_006711019.3 | 69.6% | 64.2% | 551_552ins104;591_592ins154 |
| 3 | human | 8764 | TNFRSF14 | TNF receptor superfamily me... | XM_017002720.1 | 68.6% | 66% | (many diffs) |
| 4 | human | 8764 | TNFRSF14 | TNF receptor superfamily me... | NM_001297605.1 | 65% | 65% | 552_553ins297 |
| 5 | human | 8764 | TNFRSF14 | TNF receptor superfamily me... | XM_011542383.2 | 58.3% | 34.1% | (many diffs) |
| 6 | human | 8764 | TNFRSF14 | TNF receptor superfamily me... | XR_001737513.2 | 45.1% | 1_294del;846_1024del;1323_1881del | |
| 7 | human | 8764 | TNFRSF14 | TNF receptor superfamily me... | XR_001737512.2 | 44% | (many diffs) | |
| 8 | human | 8764 | TNFRSF14 | TNF receptor superfamily me... | XR_001737511.1 | 40.3% | (many diffs) | |
| 9 | human | 8764 | TNFRSF14 | TNF receptor superfamily me... | XR_426638.3 | 30% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 918
- ORF length:
- 849
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggagcctcct ggagactggg ggcctcctcc ctggagatcc acccccaaaa 121 ccgacgtctt gaggctggtg ctgtatctca ccttcctggg agccccctgc tacgccccag 181 ctctgccgtc ctgcaaggag gacgagtacc cagtgggctc cgagtgctgc cccaagtgca 241 gtccaggtta tcgtgtgaag gaggcctgcg gggagctgac gggcacagtg tgtgaaccct 301 gccctccagg cacctacatt gcccacctca atggcctaag caagtgtctg cagtgccaaa 361 tgtgtgaccc agccatgggc ctgcgcgcga gccggaactg ctccaggaca gagaacgccg 421 tgtgtggctg cagcccaggc cacttctgca tcgtccagga cggggaccac tgcgccgcgt 481 gccgcgctta cgccaccTCC AGCCCGGGCC AGAGGGTGCA GAAGGGAGGC ACCGAGAGTC 541 AGGACACCCT GTGTCAGAAC TGCCCCCCGG GGACCTTCTC TCCCAATGGG ACCCTGGAGG 601 AATGTCAGCA CCAGACCAAG TGCAGCTGGC TGGTGACGAA GGCCGGAGCT GGGACCAGCA 661 GCTCCCACTG GGTATGGTGG TTTCTCTCAG GGAGCCTCGT CATCGTCATT GTTTGCTCCA 721 CAGTTGGCCT AATCATATGT GTGAAAAGAA GAAAGCCAAG GGGTGATGTA GTCAAGGTGA 781 TCGTCTCCGT CCAGCGGAAA AGACAGGAGG CAGAAGGTGA GGCCACAGTC ATTGAGGCCC 841 TGCAGGCCCC TCCGGACGTC ACCACGGTGG CCGTGGAGGA GACAATACCC TCATTCACGG 901 GGAGGAGCCC AAACCACTAG AACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 961 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1021 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGATC GCCCAGCTGT 1081 TAGGCCCAGC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt