Construct: ORF TRCN0000489271
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021515.1_s317c1
- DNA Barcode:
- AATAAATTAAACACGCAGCTCTCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PRKX (5613)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489271
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5613 | PRKX | protein kinase X-linked | NM_005044.5 | 99.9% | 99.7% | 128T>C |
| 2 | human | 5616 | PRKY | protein kinase Y-linked (ps... | NR_028062.1 | 13.3% | (many diffs) | |
| 3 | human | 441733 | PRKXP1 | PRKX pseudogene 1 | NR_073405.1 | 7.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1146
- ORF length:
- 1074
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaggcg cccgggctgg cccaggcggc cgcggcggag agcgactccc 121 gcaaggtggc ggaggagacc cccgacgggg cgcccgcgct ctgccccagc cctgaggcgc 181 tgtcgccgga gccgcctgcg tacagcctgc aggactttga cacgctggcc accgtgggca 241 ctgggacgtt cgggcgggtg cacctggtga aggagaagac agccaagcat ttcttcgccc 301 tcaaggtgat gagcattccc gacgtcatcc gcctaaagca ggagcaacac gtacacaatg 361 agaagtctgt cctgaaggaa gtcagccacc cgttcctcat caggctgttc tggacgtggc 421 atgacgagcg cttcctctac atgctcatgg agtacgtgcc gggcggcgag ctcttcagct 481 acctgcgcaa ccgggggcgc ttctccagca ccacggggct cttctactct gcagagatca 541 tctgtgccat cgagtacctg cactccaaag agatcgtcta cagggacttg aagccagaga 601 acatcctgct ggatagggat ggccacatta agctcacgga ctttgggttc gccaagaagc 661 tggtagacag gacttggacc ctctgtggaa cacccgagta cctagccccc gaagtcattc 721 agagcaaggg ccacggaagg gccgtggact ggtgggccct cggcatcctg atattcgaga 781 tgctttcggg gtttccTCCG TTTTTTGATG ACAACCCGTT TGGCATTTAT CAGAAAATTC 841 TTGCAGGCAA AATAGATTTC CCCAGACATT TGGATTTCCA TGTAAAAGAC CTCATTAAGA 901 AACTGCTCGT GGTTGACAGA ACAAGGCGAT TAGGAAACAT GAAGAACGGG GCGAATGATG 961 TGAAGCATCA TCGGTGGTTC CGCTCCGTGG ACTGGGAAGC TGTTCCGCAG AGAAAACTGA 1021 AGCCTCCCAT CGTGCCCAAG ATAGCTGGTG ACGGCGACAC TTCCAACTTC GAAACTTACC 1081 CTGAGAATGA CTGGGACACA GCCGCGCCCG TGCCGCAGAA GGATTTAGAA ATCTTCAAGA 1141 ATTTCTAGGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1201 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1261 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAATAAA TTAAACACGC AGCTCTCTAC 1321 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt