Construct: ORF TRCN0000489271

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021515.1_s317c1
DNA Barcode:
AATAAATTAAACACGCAGCTCTCT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
PRKX (5613)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489271
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5613 PRKX protein kinase X-linked NM_005044.5 99.9% 99.7% 128T>C
2 human 5616 PRKY protein kinase Y-linked (ps... NR_028062.1 13.3% (many diffs)
3 human 441733 PRKXP1 PRKX pseudogene 1 NR_073405.1 7.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1146
ORF length:
1074
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggaggcg cccgggctgg cccaggcggc cgcggcggag agcgactccc
121gcaaggtggc ggaggagacc cccgacgggg cgcccgcgct ctgccccagc cctgaggcgc
181tgtcgccgga gccgcctgcg tacagcctgc aggactttga cacgctggcc accgtgggca
241ctgggacgtt cgggcgggtg cacctggtga aggagaagac agccaagcat ttcttcgccc
301tcaaggtgat gagcattccc gacgtcatcc gcctaaagca ggagcaacac gtacacaatg
361agaagtctgt cctgaaggaa gtcagccacc cgttcctcat caggctgttc tggacgtggc
421atgacgagcg cttcctctac atgctcatgg agtacgtgcc gggcggcgag ctcttcagct
481acctgcgcaa ccgggggcgc ttctccagca ccacggggct cttctactct gcagagatca
541tctgtgccat cgagtacctg cactccaaag agatcgtcta cagggacttg aagccagaga
601acatcctgct ggatagggat ggccacatta agctcacgga ctttgggttc gccaagaagc
661tggtagacag gacttggacc ctctgtggaa cacccgagta cctagccccc gaagtcattc
721agagcaaggg ccacggaagg gccgtggact ggtgggccct cggcatcctg atattcgaga
781tgctttcggg gtttccTCCG TTTTTTGATG ACAACCCGTT TGGCATTTAT CAGAAAATTC
841TTGCAGGCAA AATAGATTTC CCCAGACATT TGGATTTCCA TGTAAAAGAC CTCATTAAGA
901AACTGCTCGT GGTTGACAGA ACAAGGCGAT TAGGAAACAT GAAGAACGGG GCGAATGATG
961TGAAGCATCA TCGGTGGTTC CGCTCCGTGG ACTGGGAAGC TGTTCCGCAG AGAAAACTGA
1021AGCCTCCCAT CGTGCCCAAG ATAGCTGGTG ACGGCGACAC TTCCAACTTC GAAACTTACC
1081CTGAGAATGA CTGGGACACA GCCGCGCCCG TGCCGCAGAA GGATTTAGAA ATCTTCAAGA
1141ATTTCTAGGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
1201CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
1261TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAATAAA TTAAACACGC AGCTCTCTAC
1321GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)