Construct: ORF TRCN0000489319
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021058.1_s317c1
- DNA Barcode:
- TCAAAAGATGGGAAATGATATCAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GPR50 (9248)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489319
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9248 | GPR50 | G protein-coupled receptor 50 | NM_004224.3 | 99.9% | 99.8% | 1816A>G |
| 2 | human | 9248 | GPR50 | G protein-coupled receptor 50 | XM_011531216.2 | 59.8% | 59.6% | 1_1delAins742;1075A>G |
| 3 | mouse | 14765 | Gpr50 | G-protein-coupled receptor 50 | NM_001308501.1 | 32.6% | 33.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1923
- ORF length:
- 1851
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggggccc accctagcgg ttcccacccc ctatggctgt attggctgta 121 agctacccca gccagaatac ccaccggctc taatcatctt tatgttctgc gcgatggtta 181 tcaccatcgt tgtagaccta atcggcaact ccatggtcat tttggctgtg acgaagaaca 241 agaagctccg gaattctggc aacatcttcg tggtcagtct ctctgtggcc gatatgctgg 301 tggccatcta cccataccct ttgatgctgc atgccatgtc cattgggggc tgggatctga 361 gccagttaca gtgccagatg gtcgggttca tcacagggct gagtgtggtc ggctccatct 421 tcaacatcgt ggcaatcgct atcaaccgtt actgctacat ctgccacagc ctccagtacg 481 aacggatctt cagtgtgcgc aatacctgca tctacctggt catcacctgg atcatgaccg 541 tcctggctgt cctgcccaac atgtacattg gcaccatcga gtacgatcct cgcacctaca 601 cctgcatctt caactatctg aacaaccctg tcttcactgt taccatcgtc tgcatccact 661 tcgtcctccc tctcctcatc gtgggtttct gctacgtgag gatctggacc aaagtgctgg 721 cggcccgtga ccctgcaggg cagaatcctg acaaccaact tgctgaggtt cgcaattttc 781 taaccatgtt tgtgatcttc ctcctctttg cagtgtgctg gtgccctatc aacgtgctca 841 ctgtcttggt ggctgtcagt ccgaaggaga tggcaggcaa gatccccaac tggctttatc 901 ttgcagccta cttcatagcc tacttcaaca gctgcctcaa cgctgtgatc tacgggctcc 961 tcaatgagaa tttccgaaga gaatactgga ccatcttcca tgctatgcgg caccctatca 1021 tattcttctc tggcctcatc agtgatattc gtgagatgca ggaggcccgt accctggccc 1081 gcgcccgtgc ccatgctcgc gaccaagctc gtgaacaaga ccgtgcccat gcctgtcctg 1141 ctgtggagga aaccccgatg aatgtccgga atgttccatt acctggtgat gctgcagctg 1201 gccaccccga ccgtgcctct ggccacccta agccccattc cagatcctcc tctgcctatc 1261 gcaaatctgc ctctacccac cacaagtctg tctttagcca ctccaaggct gcctctggtc 1321 acctcaagcc tgtctctggc cactccaagc ctgcctctgg tcaccccaag tctgccactg 1381 tctaccctaa gcctgcctct gtccatttca aggctgactc tgtccatttc aagggtgact 1441 ctgtccattt caagcctgac tctgttcatt tcaagcctgc ttccagcaac cccaagccca 1501 tcactggcca ccatgtctct gctggcagcc actccaagtc tgccTTCAGT GCTGCCACCA 1561 GCCACCCTAA ACCCACCACT GGCCACATCA AGCCAGCTAC CAGCCATGCT GAGCCCACCA 1621 CTGCTGACTA TCCCAAGCCT GCCACTACCA GCCACCCTAA GCCCACTGCT GCTGACAACC 1681 CTGAGCTCTC TGCCTCCCAT TGCCCCGAGA TCCCTGCCAT TGCCCACCCT GTGTCTGACG 1741 ACAGTGACCT CCCTGAGTCG GCCTCTAGCC CTGCCGCTGG GCCCACCAAG CCTGCTGCCA 1801 GCCAGCTGGA GTCTGACACC ATCGCTGACC TTCCTGACCC TACTGTAGTC ACTACCAGTA 1861 CCAATGATTA CCATGATGTC GTGGTTGTTG ATGTTGAAGA TGATCCTGAT GAAATGGCTG 1921 TGTAGAACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1981 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2041 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATCAAAAGAT GGGAAATGAT ATCAAACGCG 2101 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt